Use of ZmSBP12 gene in regulation of drought resistance, plant height, and ear height of Zea mays L

Patent metadata

Field	Value
Publication number	US-11879131-B2
Application number	US-202017907408-A
Country	US
Kind code	B2
Filing date	Oct 21, 2020
Priority date	Mar 27, 2020
Publication date	Jan 23, 2024
Grant date	Jan 23, 2024

Abstract

A use of a ZmSBP12 gene in the regulation of drought resistance, plant height, and ear height of Zea mays L. is provided. After the ZmSBP12 gene is over-expressed in Zea mays L., the resulting Zea mays L. mutant plant exhibits increased drought resistance and decreased plant and ear heights. The overexpression of the ZmSBP12 gene leads to increased drought resistance and decreased plant and ear heights, indicating that the ZmSBP12 gene plays a crucial role in the drought resistance and plant type (plant height) of Zea mays L. The expression abundance of the ZmSBP12 gene is increased to improve the drought resistance of Zea mays L. and reduce the plant and ear heights of Zea mays L., which can be used for the assisted breeding of novel drought-resistant and lodging-resistant Zea mays L. varieties and the breeding of excellent inbred lines and hybrids of Zea mays L.

First claim

What is claimed is:

1. A method for improving lodging resistance of a Zea mays plant comprising:
(i) constructing a recombinant plant expression vector carrying a ZmSBP12 coding sequence as set forth in SEQ ID NO: 2 or SEQ ID NO: 6 which encodes the ZmSBP12 protein as set forth in SEQ ID NO:1, wherein said recombinant plant expression vector comprises inserting the nucleotide sequence as set forth in SEQ ID NO: 2 or SEQ ID NO: 6 between Pstl and BamHI restriction sites of a modified pCAMBIA vector to obtain a ZmSBP12 overexpression vector pCAMBIA-Ub1::ZmSBP12-eGFP;
(ii) transforming said constructed recombinant plant expression vector into a Zea mays plant tissue or a Zea mays plant cell;
(iii) overexpressing said ZmSBP12 protein in the transformed Zea mays plant tissue or the Zea mays plant cell;
(iv) regenerating transformed Zea mays plants overexpressing said ZmSBP12 protein from said transformed Zea mays plant tissue or the Zea mays plant cell of step (iii); and
(v) selecting a transformed Zea mays plant from said regenerated transformed plants of step (iv) that overexpresses said ZmSBP12 protein and exhibits increased lodging resistance as compared to a control or wild-type Zea mays plant that is not transformed with said constructed recombinant plant expression vector and is grown under identical growth conditions.

2. The method according to claim 1 , wherein said method comprises transforming the ZmSBP12 overexpression vector pCAMBIA-Ub1::ZmSBP12-eGFP into A. tumefaciens EHA105 through electric shock to obtain a recombinant strain pCAMBIA-Ubi::ZmSBP12-eGFP/EHA105.

3. The method according to claim 2 , wherein the method further comprises transforming the recombinant strain pCAMBIA-Ub1::ZmSBP12-eGFP/EHA105 into an immature Zea mays embryo callus.

4. The method according to claim 1 , wherein the selected transformed Zea mays plant of step (iv) further exhibits reduced plant height or reduced ear height as compared to said control.

5. The method according to claim 1 , wherein the coding sequence is set forth in SEQ ID NO: 2.

6. The method according to claim 1 , wherein the coding sequence is set forth in SEQ ID NO: 6.

Assignees

• Univ South China Agricult
• Biotechnology Res Institute Chinese Academy Of Agricultural Sciences

Inventors

• Wang Haiyang
• Xie Yurong
• Zhao Bingbing
• Wang Baobao
• Zhao Yongping
• Kong Dexin
• Li Quanquan
• Li Yaoyao

Classifications

• C12N15/8273 Primary

  for drought, cold, salt resistance · CPC title

• C12N15/8262

  involving plant development · CPC title

• C07K14/415 Primary

  from plants · CPC title

• C12N15/8261

  with agronomic (input) traits, e.g. crop yield · CPC title

• C12N15/8218

  Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS] · CPC title