Modulation of transgene expression in plants

Patent metadata

Field	Value
Publication number	US-11879129-B2
Application number	US-202217931436-A
Country	US
Kind code	B2
Filing date	Sep 12, 2022
Priority date	Oct 11, 2016
Publication date	Jan 23, 2024
Grant date	Jan 23, 2024

Abstract

This disclosure concerns the use of endogenous plant RNAi machinery to preferentially or specifically reduce transgene expression. In some embodiments, the disclosure concerns specific reduction of transgene expression in seed tissues of a dicot plant.

First claim

What is claimed is:

1. A method for modulating the expression of transgene expression in tissue of plants, the method comprising the steps of:
a. Providing at least one gene expression cassette comprising a sRNA target site of SEQ ID NO:4 operably linked to a transgene into a plant; and,
b. Expressing the transgene, wherein the modulation of the transgene expression occurs in seed-coat specific plant tissue and the transgene is expressed in the other plant tissues.

2. The method of claim 1 , wherein the transgene comprises an agronomic trait gene, an insect resistance gene, an herbicide tolerance gene, a non-coding RNA gene, or a selectable marker gene.

3. The method of claim 2 , wherein the herbicide tolerant gene is aad-12.

4. The method of claim 1 , wherein the plant is a dicot plant.

5. The method of claim 4 , wherein the dicot plant is selected from the group consisting of Arabidopsis , soybean, rapeseed, cotton, and tobacco.

6. The method of claim 1 , wherein the transgene is heterologous to the plant, and wherein at least one sRNA molecule that binds to the sRNA target site is an endogenous sRNA of the plant.

7. The method of claim 6 , wherein the sRNA molecule is SEQ ID NO:3.

8. The method of claim 1 , wherein the gene expression cassette comprises a non-constitutive promoter.

9. The method of claim 1 , wherein the gene expression cassette comprises a tissue-specific promoter.

10. The method of claim 1 , wherein the gene expression cassette comprises a constitutive promoter.

11. The method of claim 1 , wherein the expressed transgene that is modulated is an herbicide tolerant transgene.

12. The method of claim 1 , wherein the gene expression cassette further comprises SEQ ID NO:7.

13. The method of claim 1 , the method further comprising the steps of:
c. Applying a herbicide to the plant tissues;
d. Obtaining plant tissues that are tolerant to the herbicide; and,
e. Regenerating a whole plant comprising the gene expression cassette, wherein the whole plant is resistant to the herbicide.

14. The method of claim 13 , wherein the herbicide comprises 2,4-D.

Assignees

• Corteva Agriscience Llc

Inventors

• Bowling Andrew J
• German Marcelo Ariel
• Glancy Todd P
• Kumar Sandeep
• Pence Heather
• Robinson Andrew E
• Sriram Shreedharan
• Wang Po-Hao
• Yerkes Carla N

Classifications

• C12N15/8218 Primary

  Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS] · CPC title

• C12N15/8223

  Vegetative tissue-specific promoters · CPC title

• C12N15/8234

  Seed-specific, e.g. embryo, endosperm · CPC title

• C12N15/8274

  for herbicide resistance · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title