Brevibacillus agri, preparation thereof, method for preparing surfactant and use thereof

Patent metadata

Field	Value
Publication number	US-11879101-B2
Application number	US-202117317812-A
Country	US
Kind code	B2
Filing date	May 11, 2021
Priority date	Aug 13, 2020
Publication date	Jan 23, 2024
Grant date	Jan 23, 2024

Abstract

The present disclosure relates to the technical field of application of bioengineering technology in microbial oil recovery, and discloses a Brevibacillus agri strain and preparation thereof and a method for preparing surfactant, and use thereof. The Brevibacillus agri strain is deposited in the China General Microbiological Culture Collection Center under the accession number CGMCC No. 9983. The Brevibacillus agri and its preparation may effectively enhance the crude oil recovery; the method for preparing the surfactant allow the lipopeptide biosurfactant to have good physical properties, effectively reduce the surface tension, and have good emulsifying performance for petroleum, various hydrocarbons and lipids.

First claim

What is claimed is:

1. A method for preparing biosurfactant, wherein the method comprises fermenting a Brevibacillus agri strain in a nutrient medium to produce a lipopeptide biosurfactant, wherein the Brevibacillus agri strain is Brevibacillus agri deposited with the China General Microbiological Culture Collection Center (CGMCC) with CGMCC deposit number 9983, wherein the nutrient medium comprises: MgSO 4 0.2 g/L, K 2 HPO 4 1.0 g/L, KH 2 PO 4 1.0 g/L, Na 2 HPO 4 4.0 g/L, NaCl 10.0 g/L, NaNO 3 10.0 g/L, crude oil 10.0 g/L and molasses 2-3 g/L, pH is 7.0, and a fermentation temperature is 25-60° C.

2. The method according to claim 1 , wherein the nutrient medium further comprises 1-5 mL of a solution of trace elements and 1-10 mL of a solution of a vitamin complex.

3. The method according to claim 1 , further comprising removing the Brevibacillus agri strain after fermentation.

4. The method according to claim 1 , further comprising obtaining the lipopeptide biosurfactant.

5. The method according to claim 1 , wherein the lipopeptide biosurfactant is a metabolite of the Brevibacillus agri strain.

6. The method according to claim 1 , wherein the Brevibacillus agri strain is a liquid bacterial preparation.

7. The method according to claim 6 , wherein the nutrient medium further comprises 1-5 mL of a solution of trace elements and 1-10 mL of a solution of a vitamin complex.

8. The method according to claim 6 , further comprising removing the Brevibacillus agri strain after fermentation.

9. The method according to claim 4 , wherein the nutrient medium further comprises 1-5 mL of a solution of trace elements and 1-10 mL of a solution of a vitamin complex.

10. The method according to claim 4 , further comprising removing the Brevibacillus agri strain after fermentation.

Assignees

• Univ Yangtze

Inventors

• She Yuehui
• Zhang Fan
• Zhang Zhi
• Yao Puyong
• Li Fei
• Dong Hao
• Sun Shanshan
• Yu Gaoming
• Yi Shaojin
• Zhang Wenda
• Hu Linqi
• Feng Yangyang
• Zheng Anying
• Li Yang

Classifications

• C10G32/00 Primary

  Refining of hydrocarbon oils by electric or magnetic means, by irradiation, or by using microorganisms · CPC title

• C12N1/205 Primary

  Bacterial isolates · CPC title

• C12R2001/01

  Bacteria or Actinomycetales {; using bacteria or Actinomycetales} · CPC title

• C12N1/20

  Bacteria; Culture media therefor · CPC title

• C12P21/00

  Preparation of peptides or proteins (single cell protein C12N1/00) · CPC title