Crystallization of steviol glycosides

Patent metadata

Field	Value
Publication number	US-11878992-B2
Application number	US-201716322410-A
Country	US
Kind code	B2
Filing date	Aug 9, 2017
Priority date	Aug 9, 2016
Publication date	Jan 23, 2024
Grant date	Jan 23, 2024

Abstract

A method for purifying rebaudioside M, which method comprises: (a) providing a solution comprising rebaudioside M at a concentration of at least about 10 g/L and at a purity of at least about 10% by weight on a dry basis; and (b) crystallizing from the solution a high purity composition comprising rebaudioside M, thereby to purify rebaudioside M.

First claim

The invention claimed is:

1. A method for purifying rebaudioside M, which method comprises:
(a) providing a solution comprising rebaudioside M, wherein the solution is a fermentation broth which has undergone a solid-liquid separation to separate cells from the fermentation broth, wherein the rebaudioside M in the solution has a concentration of at least about 10 g/L and a purity of at least 10% by weight on a dry basis; and
(b) crystallizing rebaudioside M from the solution of (a) wherein the crystallizing is carried out in water and in the absence of organic solvent, thereby obtaining purified rebaudioside M,

wherein the method is performed without chromatography, and in the absence of organic solvent.

2. The method according to claim 1 , wherein the purified rebaudioside M has a purity greater than about 90% by weight on a dry basis.

3. The method according to claim 1 , wherein the purified rebaudioside M has a purity of at least about 98% by weight on a dry basis of total steviol glycosides.

4. The method according to claim 1 , wherein the purified rebaudioside M comprises no more than about 150 ppm of kaurenoic acid equivalents on a dry weight basis.

5. The method according to claim 1 , wherein the purified rebaudioside M comprises no more than about 2% stevioside by weight on a dry weight basis.

6. The method according to claim 1 , wherein the purified rebaudioside M has a purity greater than about 60% by weight on a dry basis.

7. The method according to claim 1 , further comprising, before (b), seeding the solution with an amount of rebaudioside M crystals sufficient to promote crystallization of the rebaudioside M.

8. The method according to claim 1 , further comprising separating and washing the purified rebaudioside M.

9. The method according to claim 1 , further comprising drying the purified rebaudioside M.

10. The method according to claim 1 , wherein the purified rebaudioside M is subjected to a second purification recrystallization in water.

11. The method according to claim 1 , wherein the solution comprising rebaudioside M of (a) has a concentration of rebaudioside M of at least 15% on a dry weight basis.

12. The method according to claim 1 , wherein the solution comprising rebaudioside M of (a) has a concentration of rebaudioside M of at least 15 g/L.

13. The method of claim 1 , wherein (a) further comprises concentrating the solution comprising rebaudioside M, to yield a concentrated solution.

14. The method of claim 13 , wherein the concentrated solution comprises rebaudioside M at a concentration of at least 30 g/L.

15. The method of claim 13 , wherein the concentrated solution comprises rebaudioside M at a concentration of at least 80 g/L.

16. The method of claim 13 , wherein the concentrating comprises:
a combination of ultrafiltration and nanofiltration;
evaporation; and/or
spray-drying the solution of (a) and then redissolving the spray-dried material.

Assignees

• Dsm Ip Assets Bv

Inventors

• Galaev Igor
• De Pater Robertus Mattheus

Classifications

• C07H15/256 Primary

  Polyterpene radicals · CPC title

• A23F3/405

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• A23F5/465

  Flavouring with flavours other than natural coffee flavour or coffee oil · CPC title

• A23L2/60

  Sweeteners · CPC title

• C07H1/06

  Separation; Purification · CPC title