Delta-2-tubulin as a biomarker and therapeutic target for peripheral neuropathy

Patent metadata

Field	Value
Publication number	US-11874284-B2
Application number	US-202016884768-A
Country	US
Kind code	B2
Filing date	May 27, 2020
Priority date	Nov 29, 2017
Publication date	Jan 16, 2024
Grant date	Jan 16, 2024

Abstract

The present disclosure relates to delta-2-tubulin and its use as a biomarker for determining if a subject is at risk of developing peripheral neuropathy or if a subject has developed peripheral neuropathy. The present disclosure further relates to methods for the treatment of peripheral neuropathy in a subject and assays for identifying compounds that can be used to treat and/or prevent peripheral neuropathy.

First claim

We claim:

1. A method for the treatment of chemotherapy-induced peripheral neuropathy in a subject having a cancer, comprising:
a) obtaining a first sample from the subject before treatment with an anti-cancer agent;
b) obtaining a second sample from the subject after treatment with the anti-cancer agent;
c) determining the expression level of a delta-2-tubulin in the second sample is increased compared to the expression level in the first sample; and
d) administering an siRNA or shRNA targeting cytosolic carboxypeptidase 1 (CCP1), wherein the first sample and the second sample comprise a sample of nervous tissue.

2. The method of claim 1 , wherein the expression level of the delta-2-tubulin is measured by immunofluorescence.

3. The method of claim 1 , wherein the expression level of the delta-2-tubulin is measured by Western Blot.

Assignees

• Univ Columbia
• Univ Degli Studi Di Milano Bicocca

Inventors

• Bartolini Francesca
• Pero Maria Elena
• Cavaletti Guido

Classifications

• G01N33/575 Primary

  for cancer · CPC title

• G01N33/6896 Primary

  Neurological disorders, e.g. Alzheimer's disease · CPC title

• A61K31/69

  Boron compounds · CPC title

• A61K31/7105

  Natural ribonucleic acids, i.e. containing only riboses attached to adenine, guanine, cytosine or uracil and having 3'-5' phosphodiester links · CPC title

• A61K45/06

  Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca · CPC title