Method for producing marine algae-derived agarotriose, and use thereof as prebiotic

Patent metadata

Field	Value
Publication number	US-11872253-B2
Application number	US-202217724386-A
Country	US
Kind code	B2
Filing date	Apr 19, 2022
Priority date	Nov 16, 2017
Publication date	Jan 16, 2024
Grant date	Jan 16, 2024

Abstract

The present invention relates to a method for producing marine algae-derived agarotriose, and a use thereof as a prebiotic. More specifically, the present invention investigates the characteristics of agarotriose as a prebiotic which is selectively metabolized by probiotic microorganisms, thereby enabling agarotriose to be used as an anti-cancer or anti-inflammatory agent in the fields of food and pharmaceuticals, and enabling agarotriose to be obtained at high yield through efficient purification with minimal loss after enzymatic hydrolysis of a red algae-derived polysaccharide without pre-treatment.

First claim

What is claimed is:

1. Amethod for producing agarotriose, the method comprising:
(a) reacting any one substrate of agar, agarose or neoagarohexose with a β-agarase of SEQ ID NO:1 or SEQ ID NO:5;
(b) reacting the reaction product of step (a) with a neoagarobiose hydrolase of SEQ ID NO: 2 or SEQ ID NO:6, to make a resulting product comprising agarotriose; and
(c) purifying the agarotriose from the resulting product through a size-exclusion column.

2. The method of claim 1 , wherein the β-agarase of SEQ ID NO: 1 is an enzyme that is derived from a Bacteroides plebeius DSM 17135 strain, and that uses agar, agarose, or neoagarohexaose as a substrate to degrade the substrate into neoagarotetraose and neoagarobiose.

3. The method of claim 1 , wherein step (a) is performed at 0 to 200 rpm under a temperature condition of 30 to 60° C. for 5 minutes to 12 hours.

4. The method of claim 1 , wherein the neoagarobiose hydrolase of SEQ ID NO:2 is an enzyme that is derived from a Bacteroides plebeius DSM 17135 strain, and that uses neoagarotetraose or neoagarobiose as a substrate to degrade the substrate into agarotriose, galactose, or 3,6-anhydro-L-galactose.

5. The method of claim 1 , wherein step (b) is performed at 0 to 200 rpm under a temperature condition of 25 to 45° C. for 30 minutes to 12 hours.

Assignees

• Univ Korea Res & Bus Found

Inventors

• Kim Kyoung Heon
• Jin Yong-Su
• Yun Eun-Ju
• Kim Dong-Hyun
• Yu Sora
• Cho Kyung-Mun
• Park Na Jeong

Classifications

• A61K35/44 Primary

  Vessels; Vascular smooth muscle cells; Endothelial cells; Endothelial progenitor cells · CPC title

• A23L33/125

  containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates (indigestible substances A23L33/21) · CPC title

• A23L33/135

  Bacteria or derivatives thereof, e.g. probiotics · CPC title

• A23L33/40 Primary

  Complete food formulations for specific consumer groups or specific purposes, e.g. infant formula · CPC title

• A61K35/745

  Bifidobacteria · CPC title