Detecting an analyte

Patent metadata

Field	Value
Publication number	US-11867699-B2
Application number	US-201716308219-A
Country	US
Kind code	B2
Filing date	Jun 12, 2017
Priority date	Jun 10, 2016
Publication date	Jan 9, 2024
Grant date	Jan 9, 2024

Abstract

This invention provides a method for detecting an analyte in a sample, including a step of contacting said analyte with a nanoparticle to facilitate binding thereto, wherein the nanoparticle comprises: (i) a core; (ii) pores extending radially from said core and being defined by spaces between an array of dendritic spikes radiating outwardly from a surface of the core, wherein the pores have an average pore size of between about 10 nm and about 20 nm; (iii) a binding agent for binding the analyte; and (iv) a detection agent immobilized within said pores; to thereby detect said analyte. This invention also provides kits, compositions and products comprising said nanoparticle.

First claim

The invention claimed is:

1. A method for detecting an analyte in a sample, including a step of contacting said analyte with a nanoparticle to facilitate binding thereto, wherein the nanoparticle comprises:
(i) a core;
(ii) pores extending radially from said core and being defined by spaces between an array of dendritic spikes radiating outwardly from a surface of the core, wherein the pores have an average pore size of between about 10 nm and about 20 nm;
(iii) a binding agent for binding the analyte; and
(iv) a detection agent immobilized within said pores;

to thereby detect said analyte.

2. The method of claim 1 , wherein the nanoparticle is a silica nanoparticle.

3. The method of claim 1 , wherein the pores are substantially conical.

4. The method of claim 1 , wherein the nanoparticle has an average particle size of between about 50 nm and about 250 nm.

5. The method of claim 1 , further including a step of producing a reporter signal from the detection agent.

6. The method of claim 5 , further including a step of detecting the reporter signal.

Assignees

• Univ Queensland

Inventors

• Yu Chengzhong
• Yu Meihua
• Lei Chang
• Kalantari Mohammad

Classifications

• G01N33/588 Primary

  with semiconductor nanocrystal label, e.g. quantum dots · CPC title

• C01B32/15

  Nano-sized carbon materials · CPC title

• C01B33/113

  Silicon oxides; Hydrates thereof {(preparing monoxide by reduction of siliceous material C01B33/182)} · CPC title

• C01B33/18

  Preparation of finely divided silica neither in sol nor in gel form; After-treatment thereof (preparation of aerogels by dehydrating gels C01B33/158; treatment to enhance the pigmenting or filling properties C09C) · CPC title

• C01B33/185

  of crystalline silica-polymorphs having molecular sieve properties, e.g. silicalites · CPC title