Method and apparatus for freezing dispensed droplets of liquid

Patent metadata

Field	Value
Publication number	US-11867460-B2
Application number	US-201915734935-A
Country	US
Kind code	B2
Filing date	Jun 14, 2019
Priority date	Jun 20, 2018
Publication date	Jan 9, 2024
Grant date	Jan 9, 2024

Abstract

A method and apparatus for freezing a liquid droplet includes dispensing, by a liquid dispenser (14), a droplet (13) of liquid into a fluid chamber (10) containing a freezing fluid (12). The droplet of liquid is allowed to dwell in the freezing fluid for at least a predetermined dwell time so that the droplet of liquid freezes to a frozen droplet. The method and apparatus further includes injecting, by a gas injector (17), a stream (16) of gas transversely to a surface of the freezing fluid at about where the frozen droplet is located along the surface of the freezing fluid contained in the fluid chamber so that the frozen droplet sinks in the freezing fluid.

First claim

The invention claimed is:

1. An apparatus comprising:
a fluid chamber containing a freezing liquid;
a liquid dispenser disposed above a surface of the freezing liquid contained in the fluid chamber and configured to dispense a droplet of liquid onto the surface of the freezing liquid contained in the fluid chamber;
a gas injector disposed above the surface of the fluid contained in the fluid chamber and configured to inject a stream of gas transversely to the surface of the freezing liquid contained in the fluid chamber; and
a transporter configured to transport the fluid chamber between a first position below the liquid dispenser and a second position below the gas injector, and wherein the liquid dispenser is configured to dispense the droplet of liquid onto the surface of the freezing liquid contained in the fluid chamber when the fluid chamber is in the first position, and the gas injector is configured to inject the stream of gas transversely to the surface of the freezing liquid contained in the fluid chamber when the fluid chamber is in the second position.

2. The apparatus of claim 1 , wherein the transporter comprises a carousel configured to move the fluid chamber about an axis of rotation between the first position and the second position.

3. The apparatus of claim 2 , wherein the carousel comprises a drum and the fluid chamber is disposed within the drum.

4. The apparatus of claim 3 , wherein the carousel comprises a spindle configured to rotate about the axis of rotation, and the spindle is coupled to the drum such that the drum is configured to rotate with the spindle about the axis of rotation, and wherein rotation of the drum places different portions of the surface of the freezing liquid contained within the drum at the respective first and second positions.

5. The apparatus of claim 3 , wherein the carousel comprises a lid coupled to a top end of the drum, wherein the fluid chamber is disposed beneath the lid, and the lid comprises an opening aligned with the fluid chamber.

6. The apparatus of claim 3 , wherein the carousel comprises an insulation layer disposed between the drum and the fluid chamber.

7. The apparatus of claim 3 , wherein the carousel comprises a strainer basket disposed in the drum, and the strainer basket is configured to receive and hold a frozen droplet sinking toward a bottom of the fluid chamber.

8. The apparatus of claim 1 , comprising multiple fluid chambers, wherein the transporter is configured to transport each fluid chamber between the first position below the liquid dispenser and the second position below the gas injector, and comprising multiple gas injectors, wherein the transporter is configured to transport each fluid chamber to the second position below a respective one of the gas injectors.

9. The apparatus of claim 1 , wherein the gas injector comprises a nozzle disposed above the fluid chamber, and wherein the nozzle is configured to inject the gas stream transversely to the surface of the freezing liquid contained in the fluid chamber, and wherein the apparatus further comprises:
a solenoid valve controlling gas flow to the nozzle and configured to switch between a closed position to shut-off the gas stream from reaching the nozzle and an open position to permit the gas stream to reach the nozzle;
a sensor configured to generate a signal relating to a position of the transporter; and
a control unit in electrical communication with the sensor and the solenoid valve, wherein the control unit is configured to receive the signal from the sensor and transmit a command to the solenoid valve to switch between the open and closed positions based on the signal.

10. The apparatus of claim 1 , wherein the transporter is configured to transport the fluid chamber from the first position to the second position at a predetermined dwell time so that the droplet of liquid freezes to a frozen droplet before the gas injector injects the stream of gas transversely to the surface of the freezing liquid contained in the fluid chamber.

11. A method comprising: (a) dispensing, by a liquid dispenser located above a surface of a freezing fluid, a droplet of liquid into a fluid chamber containing the freezing fluid; (b) after step (a), allowing the droplet of liquid to dwell in the freezing fluid for at least a predetermined dwell time so that the droplet of liquid freezes to a frozen droplet; (c) after step (a), transporting the fluid chamber by a transporter from a first position below the liquid dispenser to a second position below a gas injector, and (d) after steps (b) and (c), injecting a stream of gas, by the gas injector located above the surface of the freezing fluid, transversely to the surface of the freezing fluid toward where the frozen droplet is located along the surface of the freezing fluid so that the frozen droplet sinks in the freezing fluid.

12. The method of claim 11 , wherein step (d) further comprises monitoring the predetermined dwell time and automatically injecting the stream of gas transversely to the surface of the freezing fluid after the predetermined dwell time.

13. The method of claim 11 ,
wherein step (d) further comprises monitoring a position of the transporter and automatically injecting the stream of gas when the fluid chamber is at the second position beneath the gas injector.

14. The method of claim 13 , further comprising: after step (d), transporting the fluid chamber by the transporter from the second position below the gas injector back to the first position below the liquid dispenser; and
after the fluid chamber returns back to the first position, dispensing a second droplet of liquid into the fluid chamber.

15. The method of claim 11 , further comprising:
before step (a), positioning the liquid dispenser over the fluid chamber to align the liquid dispenser with a target zone located along the surface of the freezing fluid contained in the fluid chamber;
wherein step (a) further comprises dispensing, by the liquid dispenser, the droplet of liquid at the target zone,
wherein step (c) comprises transporting the fluid chamber by the transporter to the second position to align the gas injector with the target zone located along the surface of the freezing fluid contained in the fluid chamber, and
wherein step (d) further comprises injecting, by the gas injector, the stream of gas at the target zone.

16. The method of claim 11 , further comprising:
after step (d), collecting, by a retainer basket, the frozen droplet sinking toward a bottom of the fluid chamber; and
after the step of collecting the frozen droplet, drying the frozen droplet.

17. An apparatus comprising:
a fluid chamber containing a freezing liquid;
a liquid dispenser; and
a gas injector;
wherein the liquid dispenser is configured to move relative to the fluid chamber so that the liquid dispenser is aligned above a target zone located along a surface of the freezing liquid contained in the fluid chamber, and the liquid dispenser is configured to dispense a droplet of liquid onto the surface of the freezing liquid contained in the fluid chamber at the target zone;
wherein the gas injector is configured to move relative to the fluid chamber so that the gas injector is aligned above the target zone located along the surface of the freezing liquid contained in the fluid chamber, and the gas injector is configured to inject a gas stream transversely to the surface of the freezing liquid contained in the fluid chamber at the target zone.

18. The apparatus of claim 17 , wherein the liquid dispenser is configured to

Assignees

• Gen Probe Inc

Inventors

• Bashar Reza

Classifications

• A01N1/144

  for temperature control, e.g. refrigerators or freeze-drying apparatus · CPC title

• F25D3/11 Primary

  with conveyors carrying articles to be cooled through the cooling space · CPC title

• A01N1/0252

  Human Necessities · mapped topic

• B01J2/06

  in a liquid medium · CPC title

• G01N1/42 Primary

  Low-temperature sample treatment, e.g. cryofixation · CPC title