Sensor, detection method, reagent and kit
US-2022081702-A1 · Mar 17, 2022 · US
Sensor, detection method, reagent and kit
US11859235B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11859235-B2 |
| Application number | US-202117199380-A |
| Country | US |
| Kind code | B2 |
| Filing date | Mar 11, 2021 |
| Priority date | Sep 15, 2020 |
| Publication date | Jan 2, 2024 |
| Grant date | Jan 2, 2024 |
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Abstract
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According to one embodiment, a sensor for detecting a target substance in gas a sample includes a target substance uptake unit that brings an acetylcholine aqueous solution into contact with a gas sample to dissolve a target substance in the gas sample into the acetylcholine aqueous solution, a reaction unit that holds acetylcholinesterase and brings the solution delivered from the target substance uptake unit into contact with the acetylcholinesterase, and a detection unit that measures a change in an amount of acetylcholine decomposition product produced in the solution delivered from the reaction unit.
First claim
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What is claimed is: 1. A sensor for detecting limonene as a target substance in a gas sample, comprising: a target substance uptake unit including a container that holds an acetylcholine aqueous solution, wherein the target substance uptake unit brings the acetylcholine aqueous solution in the container into contact with the gas sample to dissolve a target substance in the gas sample into the acetylcholine aqueous solution; a reaction unit including a reaction vessel that holds acetylcholinesterase and wherein the reaction vessel is connected to the container of the target substance uptake unit and the reaction unit brings the solution delivered from the target substance uptake unit into contact with the acetylcholinesterase; and a detection unit connected to the reaction vessel of the reaction unit, wherein the detection unit measures a change in an amount of acetylcholine decomposition product produced in the solution delivered from the reaction unit. 2. A sensor for detecting limonene as a target substance in a gas sample, comprising: a target substance uptake unit including a container that holds an acetylcholinesterase aqueous solution, wherein the target substance uptake unit brings the acetylcholinesterase aqueous solution into contact with the gas sample to dissolve a target substance in the gas sample into the acetylcholinesterase aqueous solution; a supply unit including a liquid storage unit that holds an acetylcholine aqueous solution, a reaction unit including a reaction vessel connected to the container of the target substance uptake unit and the liquid storage unit of the supply unit, wherein the reaction unit mixes the solution delivered from the target substance uptake unit and the acetylcholine aqueous solution supplied from the supply unit; and a detection unit connected to the reaction vessel of the reaction unit, wherein the detection unit measures a change in an amount of acetylcholine decomposition product produced in the solution delivered from the reaction vessel of the reaction unit. 3. The sensor according to claim 1 , wherein the acetylcholinesterase is derived from electric eel. 4. The sensor according to claim 1 , further comprising a heater for heating the reaction vessel of the reaction unit. 5. The sensor according to claim 1 , wherein the detection unit comprises a pH detection element. 6. The sensor according to claim 5 , wherein the pH detection element is a sensing element for high mobility measurement. 7. The sensor according to claim 6 , wherein the sensing element for high mobility measurement is a graphene FET. 8. A kit comprising the sensor according to claim 1 and a reagent containing acetylcholine and a reagent containing acetylcholinesterase. 9. A kit comprising the sensor according to claim 2 and a reagent containing acetylcholine and a reagent containing acetylcholinesterase. 10. The sensor according to claim 2 , wherein the acetylcholinesterase is derived from electric eel. 11. The sensor according to claim 2 , further comprising a heater for heating the reaction vessel of the reaction unit. 12. The sensor according to claim 2 , wherein the detection unit comprises a pH detection element.
Assignees
Inventors
Classifications
- C12Q1/46Primary
involving cholinesterase · CPC title
Carboxylic ester hydrolases (3.1.1) · CPC title
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Frequently asked questions
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- What does patent US11859235B2 cover?
- According to one embodiment, a sensor for detecting a target substance in gas a sample includes a target substance uptake unit that brings an acetylcholine aqueous solution into contact with a gas sample to dissolve a target substance in the gas sample into the acetylcholine aqueous solution, a reaction unit that holds acetylcholinesterase and brings the solution delivered from the target subst…
- Who is the assignee on this patent?
- Toshiba Kk
- What technology area does this patent fall under?
- Primary CPC classification C12Q1/46. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jan 02 2024 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 5 related publications on this page (citations in our corpus or others sharing the same primary CPC).