Methods and systems for preparing and preserving a biological sample

Patent metadata

Field	Value
Publication number	US-11856945-B2
Application number	US-201816606482-A
Country	US
Kind code	B2
Filing date	Apr 20, 2018
Priority date	Apr 21, 2017
Publication date	Jan 2, 2024
Grant date	Jan 2, 2024

Abstract

Methods and systems for preparing and preserving biological samples are disclosed herein. A method comprises contacting a preserving agent with a biological sample to form a mixture, wherein the preserving agent is selected from at least one of a metal-organic framework (MOF) encapsulant or a precursor forming a MOF encapsulant, and wherein the biological sample comprises at least one target analyte. A system comprises a preserving agent selected from at least one of a metal-organic framework (MOF) encapsulant or a precursor forming a MOF encapsulant; and a substrate configured to receive a drop cast mixture of the preserving agent and a biological sample, wherein the biological sample comprises at least one target analyte.

First claim

What is claimed is:

1. A method of preparing a biological sample comprising:
contacting a preserving agent with a biological sample to form a mixture, wherein the preserving agent is selected from at least one of a metal-organic framework (MOF) encapsulant or a precursor forming a MOF encapsulant, and wherein the biological sample comprises a biofluid comprising at least one target analyte; and
drop casting the mixture onto a fibrous substrate;
wherein the fibrous substrate is made of paper or cellulose; and
wherein a crystal interface is created between the protein and the fibrous substrate.

2. The method according to claim 1 , wherein the MOF is selected from the group consisting of a zeolitic imidazolate framework (ZIF) type MOF and a Materials of Institut Lavoisier (MIL) type MOF.

3. The method according to claim 1 , wherein the precursor forming the MOF encapsulant comprises 2-methylimidazole and zinc acetate.

4. The method according to claim 3 , wherein a molar ratio of 2-methylimidazole to zinc acetate is in a range of from about 4:1 to about 40:1.

5. The method according to claim 1 , wherein the substrate comprises a water insoluble material.

6. The method according to claim 1 , further comprising drying the mixture on the substrate.

7. The method according to claim 1 , further comprising storing the substrate:
at a temperature of from about −20° C. to about 100° C.; and
for at least 1 week, at least 2 weeks, at least 3 weeks, at least 4 weeks, at least 6 months, at least 1 year, or at least 5 years.

8. The method according to claim 1 , further comprising recovering the at least one target analyte from the substrate using an elution buffer, wherein the elution buffer has a pH of about 6 or less.

9. The method according to claim 1 , wherein the biological sample comprises a biofluid selected from the group consisting of urine, blood, serum, plasma, saliva, and cerebrospinal fluid.

10. The method according to claim 1 , wherein the at least one target analyte comprises at least one of a biomarker, a protein biomarker, a protein therapeutic, an antibody, a viral protein, an oligonucleotide, DNA, RNA, a macromolecule having a primary structure and a secondary structure, a protein having an amino acid sequence from an organism, and a polypeptide having an amino acid sequence from an organism;
wherein the protein biomarker is selected from the group consisting of neutrophil gelatinase-associated lipocalin (NGAL), kidney injury molecule-1 (KIM-1), albumin, beta-2 microglobulin, cystatin C, cancer antigen 125 (CA-125), prostate-specific antigen (PSA), human IgG and IgM, ZIKV nonstructural protein 1, and cytokines; and
wherein the protein therapeutic is selected from the group consisting of insulin, monoclonal antibodies, erythropoietin, cytokines, and vaccines.

Assignees

• Singamaneni Srikanth
• Wang Congzhou
• Morrissey Jeremiah J
• Kharasch Evan D
• Washington University St Louis

Inventors

• Singamaneni Srikanth
• Wang Congzhou
• Morrissey Jeremiah J
• Kharasch Evan D

Classifications

• A01N1/128 Primary

  Chemically defined matrices for immobilising, holding or storing living parts, e.g. alginate gels; Chemically altering living parts, e.g. by cross-linking · CPC title

• A01N1/0231 Primary

  Human Necessities · mapped topic

• G01N1/4044

  by chemical techniques; Digestion; Chemical decomposition · CPC title

• G01N1/36 Primary

  Embedding or analogous mounting of samples · CPC title

• C12N15/10

  Processes for the isolation, preparation or purification of DNA or RNA (chemical preparation of DNA or RNA C07H21/00; preparation of non-structural polynucleotides from microorganisms or with enzymes C12P19/34) · CPC title