Method for the biotechnological production of the blue-green fungus pigment xylindein

Patent metadata

Field	Value
Publication number	US-11851693-B2
Application number	US-201917276820-A
Country	US
Kind code	B2
Filing date	Nov 4, 2019
Priority date	Nov 8, 2018
Publication date	Dec 26, 2023
Grant date	Dec 26, 2023

Abstract

The invention relates to a method for the biotechnological production of the blue-green fungus pigment xylindein from fungus biomass in a bioreactor, the reactor contents being inoculated with biomass that is uncoloured.The invention also relates to the use of uncoloured biomass of the fungus culture Chlorociboria sp. for inoculation in the biotechnological production of the blue-green fungus pigment xylindein.

First claim

We claim:

1. A method for the biotechnological production of the blue-green fungus pigment xylindein in a bioreactor, comprising the steps of
(a) bringing the reactor contents into contact with a biomass of the fungus
culture Chlorociboria sp. and stirring the reactor contents to form xylindein-containing biomass,

(b) separating the xylindein-containing biomass which formed in step (a), and
(c) extracting the xylindein from the biomass separated in step (b) using a solvent, wherein the biomass with which contact is made in step (a) is an uncoloured biomass.

2. The method according to claim 1 , wherein the solvent in step (c) is selected from the group consisting of acetone, 2-butanone and a mixture thereof.

3. The method according to claim 1 , wherein after the separation of the xylindein-containing biomass in step (b), dry and/or comminution of the biomass takes place and before step (c).

4. The method according to claim 1 , wherein the reactor contents in step (a) comprise a nutrient medium which contains up to 50 vol % food residues.

5. The method according to claim 1 , wherein the reactor contents in step (a) comprise a nutrient medium which contains a 1 vol % to 20 vol % orange juice solution.

6. The method according to claim 1 , wherein the xylindein obtained in step (c) is purified by the additional steps of (d) drying the xylindein and redissolving it in a water-soluble organic solvent, and (e) precipitating purified xylindein by adding water to the mixture from step (d).

7. The method according to claim 1 , wherein further xylindein is isolated by ultrafiltration of the liquid culture supernatant remaining in step (b) after separation of the biomass.

8. The method according to claim 1 , wherein the fungus culture Chlorociboria sp. is selected from the group consisting of Chlorociboria aeruginascens and Chlorociboria aeruginosa.

Assignees

• Univ Dresden Tech

Inventors

• Steudler Susanne
• Stange Stephanie
• Delenk Hubertus
• Wagenfuehr Andre
• Zeeb Christof
• Walther Thomas

Classifications

• C12P17/181 Primary

  Heterocyclic compounds containing oxygen atoms as the only ring heteroatoms in the condensed system, e.g. Salinomycin, Septamycin · CPC title

• C12N1/145

  Fungi isolates · CPC title

• C12R2001/645

  Fungi {; Processes using fungi} · CPC title

• C12P7/66 Primary

  containing the quinoid structure · CPC title

• C12N1/14

  Fungi (culture of mushrooms A01G18/00; as new plants A01H15/00); Culture media therefor · CPC title