Solution for extraction of RNA

Patent metadata

Field	Value
Publication number	US-11851646-B2
Application number	US-201816048695-A
Country	US
Kind code	B2
Filing date	Jul 30, 2018
Priority date	Aug 18, 2010
Publication date	Dec 26, 2023
Grant date	Dec 26, 2023

Abstract

A solution for extracting substantially pure RNA from a biological sample is disclosed. The solution for extracting RNA from a biological sample containing RNA and at least DNA comprises:(a) phenol in an amount of more than 50% by volume based on the total amount of the solution;(b) a polyol in an amount of 3 to 10% by volume based on the total amount of the solution;(c) a guanidinium salt at a concentration of 0.5 to 2.0 M based on the total amount of the solution;(d) a thiocyanate at a concentration of 0.1 to 0.5 M based on the total amount of the solution; and(e) a buffer for maintaining the pH of the solution at 4 to 6.

First claim

What is claimed is:

1. A solution for extracting RNA from a biological sample containing RNA and at least DNA, said solution comprising:
(a) phenol in an amount of not less than 53% by volume based on the total amount of said solution;
(b) a polyol in an amount of 3 to 10% by volume based on the total amount of said solution;
(c) a guanidinium salt at a concentration of 0.5 to 2.0 M based on the total amount of said solution;
(d) a thiocyanate at a concentration of 0.1 to 0.5 M based on the total amount of said solution; and
(e) a buffer for maintaining the pH of said solution at 4 to 6.

2. The solution according to claim 1 , wherein the phenol concentration is 55 to 65% by volume based on the total amount of said solution.

3. The solution according to claim 1 , further comprising an organic solvent for separating an aqueous layer.

4. The solution according to claim 1 , wherein said biological sample is a culture liquid of cultured cells.

5. The solution according to claim 1 , wherein said biological sample is a body fluid component of an organism.

6. The solution according to claim 1 , wherein said biological sample is a blood component of an organism.

Assignees

• Toray Industries

Inventors

• Yoshimoto Makiko
• Akiyama Hideo
• Nobumasa Hitoshi

Classifications

• C12N15/1003 Primary

  Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor · CPC title

• G01N1/286

  involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising (microtomes G01N1/06; pulverising in general B02C; mixing in general B01F) · CPC title

• G01N2001/2866

  Grinding or homogeneising · CPC title

• C01C3/20

  Thiocyanic acid; Salts thereof {(C01C3/001 takes precedence)} · CPC title

• C07C39/04

  Phenol · CPC title