Determining a presence of auto-fluorescent biological substances through an article

Patent metadata

Field	Value
Publication number	US-11850118-B2
Application number	US-202217843186-A
Country	US
Kind code	B2
Filing date	Jun 17, 2022
Priority date	Aug 16, 2019
Publication date	Dec 26, 2023
Grant date	Dec 26, 2023

Abstract

Techniques are disclosed for detecting a presence of a biological substance through an article such as a diaper. For example, a detection system causes a light source to transmit light through the article. The light includes a peak wavelength that corresponds to an excitation wavelength of a biological substance that may be present in the article. The detection system detects a measurement of light intensity within a range of emission wavelengths of the biological substance. By comparing the measurement of light intensity to a threshold, the detection system identifies a presence of the biological substance on the article.

First claim

That which is claimed is:

1. A method comprising:
causing, at a first time, a first light source to transmit light that includes a first peak wavelength that corresponds to a first excitation wavelength of a substance, the substance comprising multiple excitation wavelengths;
obtaining a first measurement of light intensity within a first range of emission wavelengths of the substance;
causing, at a second time, a second light source to transmit light that includes a second peak wavelength that corresponds to a second excitation wavelength of the substance;
obtaining a second measurement of light intensity within a second range of emission wavelengths; and
responsive to comparing a ratio of the first measurement of light intensity and the second measurement of light intensity against a threshold, identifying a presence of the substance.

2. The method of claim 1 , wherein the first peak wavelength is different from the second peak wavelength.

3. The method of claim 1 , wherein the first excitation wavelength is different from the second peak wavelength.

4. The method of claim 1 , wherein identifying the presence of the substance further comprises comparing the first measurement of light intensity to a first threshold and comparing the second measurement of light intensity to a second threshold.

5. The method of claim 1 , wherein the substance is a biological substance.

6. The method of claim 1 , wherein the first excitation wavelength and the second excitation wavelength are between 405 and 720 nm.

7. The method of claim 1 , wherein the first range of emission wavelengths does not overlap with the second range of emission wavelengths.

8. The method of claim 1 , wherein the first range of emission wavelengths comprises at least one wavelength in common with the second range of emission wavelengths.

9. The method of claim 1 , further comprising:
obtaining a baseline measurement of light intensity comprising a contribution of ambient light; and
removing, from the first measurement of light intensity and from the second measurement of light intensity, the baseline measurement of light intensity.

10. A method comprising:
causing a light source to transmit light that includes a peak excitation wavelength that corresponds to an excitation wavelength of a biological substance;
obtaining a measurement of light intensity within a first range of emission wavelengths of the biological substance, wherein the biological substance has multiple emission wavelengths and wherein the first range of emission wavelengths has a first peak emission wavelength;
obtaining an additional measurement of light intensity within a second range of emission wavelengths of the biological substance, wherein the second range of emission wavelengths has a second peak emission wavelength that is different than the first peak emission wavelength; and
responsive to comparing the measurement of light intensity against a threshold and comparing the additional measurement of light intensity against an additional threshold, identifying a presence of the biological substance.

11. The method of claim 10 , wherein the peak excitation wavelength is between 405 and 650 nm and the first range of emission wavelengths and the second range of emission wavelengths are 620 to 640 nm or 650 to 710 nm.

12. The method of claim 10 , further comprising:
obtaining a baseline measurement of light intensity comprising a contribution of ambient light; and
removing, from the measurement of light intensity and the additional measurement of light intensity, the baseline measurement of light intensity.

13. The method of claim 10 , wherein the biological substance is a porphyrin.

14. A system comprising:
a first light source configured to emit light that includes a first peak wavelength that corresponds to a first excitation wavelength of a substance;
a second light source configured to emit light that includes a second peak wavelength that corresponds to a second excitation wavelength of the substance;
a photodetector configured to measure an intensity of light within a range of emission wavelengths of the substance; and
a processor configured to perform operations comprising:
causing the first light source to transmit light at a first time;
obtaining a first measurement of light intensity at the photodetector at a second time that is different from the first time;
causing the second light source to transmit light at a third time different from the second time;
obtaining a second measurement of light intensity at the photodetector at a fourth time different from the third time; and
responsive to comparing a ratio of the first measurement of light intensity and the second measurement of light intensity against a threshold, identifying a presence of the sub stance.

15. The system of claim 14 , wherein the second peak wavelength is different from the first peak wavelength.

16. The system of claim 14 , the operations further comprising detecting an additional measurement of light intensity within an additional range of emission wavelengths of the substance, and wherein the identifying further comprises comparing the additional measurement of light intensity against an additional threshold.

17. The system of claim 14 , wherein identifying the presence of the substance further comprises:
comparing the first measurement of light intensity to a first threshold;
comparing the second measurement of light intensity to a second threshold; and
calculating an additional ratio of the first measurement of light intensity to the second measurement of light intensity and comparing the ratio to a third threshold.

18. The system of claim 14 , wherein the operations further comprise:
obtaining, from the photodetector, a baseline measurement of light intensity comprising a contribution of ambient light; and
removing, from the first measurement of light intensity and from the second measurement of light intensity, the baseline measurement of light intensity.

19. The system of claim 14 , wherein the second measurement of light intensity and the first measurement of light intensity represent non-overlapping ranges of wavelengths.

Assignees

• Verily Life Sciences Llc

Inventors

• Schiffer Brian

Classifications

• A61F13/42 Primary

  with wetness indicator or alarm · CPC title

• G01N21/64

  Fluorescence; Phosphorescence · CPC title

• G01N21/6428

  Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" (in vivo A61B5/00; immunoassay G01N33/53) · CPC title

• G01N21/6486

  Measuring fluorescence of biological material, e.g. DNA, RNA, cells (G01N21/6428 takes precedence) · CPC title

• G01V8/12

  using one transmitter and one receiver · CPC title