Klebsiella pneumoniae strain inducing inflammation in liver

Patent metadata

Field	Value
Publication number	US-11845926-B2
Application number	US-202017130570-A
Country	US
Kind code	B2
Filing date	Dec 22, 2020
Priority date	Apr 23, 2018
Publication date	Dec 19, 2023
Grant date	Dec 19, 2023

Abstract

To identify a microorganism causing the development of primary sclerosing cholangitis associated with ulcerative colitis. A Klebsiella pneumoniae strain inducing inflammation in the liver.

First claim

What is claimed is:

1. A method of identifying a microorganism that causes the development of primary sclerosing cholangitis in a subject, the method comprising
culturing a fecal sample collected from the subject and counting the number of cells of the microorganism, wherein the microorganism is Klebsiella pneumoniae,
determining the proportion of Th17 cells in a liver tissue obtained from the subject and correlating the number of cells of the Klebsiella pneumoniae in the fecal sample to the proportion of the Th17 cells in the liver tissue by Spearman rank-order correlation test,
detecting and counting the number of pores formed per mm 2 unit area of a large intestinal epithelium tissue obtained from the subject using a scanning electron microscope, and
wherein the presence of a correlation between the number of cells of the Klebsiella pneumoniae in the fecal sample of the subject and the proportion of the Th17 cells in the liver tissue of the subject combined with the formation of at least 40 pores with a pore size of 10 m or more per mm 2 unit area of the large intestinal epithelium tissue identifies the Klebsiella pneumoniae as the microorganism that causes the development of primary sclerosing cholangitis.

2. The method of claim 1 , wherein the Klebsiella pneumoniae has type 6 secretion system (T6SS).

3. The method of claim 1 , wherein the Klebsiella pneumoniae is the Klebsiella pneumoniae strain having the NITE deposit number BP-02879.

4. The method of claim 1 , further comprising identifying additional microorganisms that cause the development of primary sclerosing cholangitis in the subject comprising
culturing the fecal sample and counting the number of cells of the additional microorganisms, wherein the additional microorganisms are Proteus mirabilis and Enterococcus gallinarum,
correlating the number of cells of the Klebsiella pneumoniae , the Proteus mirabilis and the Enterococcus gallinarum in the fecal sample to the proportion of the Th17 cells in the liver tissue by Spearman rank-order correlation test, and
wherein the presence of a correlation between the number of cells of the Klebsiella pneumoniae , the number of cells of the Proteus mirabilis , and the number of cells of the Enterococcus gallinarum in the fecal sample of the subject and the proportion of the Th17 cells in the liver tissue of the subject combined with the formation of said pores in the large intestinal epithelium tissue identifies the Klebsiella pneumoniae , the Proteus mirabilis , and the Enterococcus gallinarum as the microorganisms that cause the development of primary sclerosing cholangitis.

5. The method of claim 4 , wherein the Proteus mirabilis is the Proteus mirabilis strain having the NITE deposit number BP-02923 and the Enterococcus gallinarum is the Enterococcus gallinarum strain having the NITE deposit number BP-02922.

Assignees

• Univ Keio

Inventors

• Nakamoto Nobuhiro
• Sasaki Nobuo
• Aoki Ryo
• Miyamoto Kentaro
• Sato Toshiro
• Kanai Takanori

Classifications

• C12N1/205 Primary

  Bacterial isolates · CPC title

• A01K67/027

  New or modified breeds of vertebrates · CPC title

• C12N1/20

  Bacteria; Culture media therefor · CPC title

• A01K2227/105

  Murine · CPC title

• A01K2267/0387

  Animal model for diseases of the immune system · CPC title