Sample injection with fluidic connection between fluid drive unit and sample accommodation volume

Patent metadata

Field	Value
Publication number	US-11835495-B2
Application number	US-202117566330-A
Country	US
Kind code	B2
Filing date	Dec 30, 2021
Priority date	May 30, 2016
Publication date	Dec 5, 2023
Grant date	Dec 5, 2023

Abstract

An injector, for injecting a fluidic sample into a flow path between a fluid drive and a sample separation unit, includes a sample accommodation volume, a sample drive, and a fluidic valve switchable to selectively couple the volume with the flow path or decouple the volume from the flow path. In an injection switching state, the fluid drive, the separation unit and the sample drive are coupled by the valve so that fluid driven by the sample drive and flowing from the volume to the separation unit and further fluid driven by the fluid drive and flowing from the fluid drive to the separation unit are combined at a fluidic connection upstream of the separation unit. A control unit controls a pressure of the fluid and/or the further fluid during injecting.

First claim

The invention claimed is:

1. An injector for injecting a fluidic sample into a flow path between a fluid drive driving a mobile phase and a separation unit, the injector comprising:
a sample accommodation volume configured to accommodate the fluidic sample prior to injecting; and
a fluidic valve switchable between multiple switching states to thereby selectively couple the sample accommodation volume with the flow path or decouple the sample accommodation volume from the flow path, wherein:
in a first injection switching state of the fluidic valve, the fluid drive, the separation unit, and the sample accommodation volume are fluidically coupled by the fluidic valve so that a sampling flow, containing at least a portion of the accommodated fluidic sample, and the mobile phase are combined at a fluidic coupling point upstream of the separation unit, thereby forming a combined fluid; and
in a second injection switching state of the fluidic valve, the sample accommodation volume is fluidically coupled by the fluidic valve between the fluid drive and the separation unit.

2. The injector of claim 1 , wherein the fluidic coupling point is located in an interior of the fluidic valve.

3. The injector of claim 1 , wherein the fluidic valve is a rotatable fluidic valve having a rotor and a stator being rotatable relative to one another to bring different fluid ports of the stator in alignment with at least one fluidic conduit in the rotor.

4. The injector of claim 3 , wherein the fluidic coupling point is at least partially defined by one fluid port being fluidically coupled to one fluid conduit at a central position of the one fluid conduit in the first injection switching state, wherein the one fluid port is further fluidically connected to a capillary guiding towards the separation unit.

5. The injector according to claim 1 , wherein, in the second injection switching state, the fluidic sample is injected towards the separation unit driven by the fluid drive while the sample accommodation volume is located downstream of the fluid drive and upstream of the separation unit.

6. The injector of claim 1 , comprising a control unit configured to control switching of the fluidic valve to select one of the first injection switching state and the second injection switching state.

7. The injector of claim 1 , comprising a control unit configured to control a pressure while the fluidic valve is in the first injection state, the pressure selected from the group consisting of: the pressure of the sampling flow upstream to the fluidic coupling point; the pressure of the mobile phase upstream to the fluidic coupling point; and the pressure of the combined fluid downstream to the fluidic coupling point.

8. The injector of claim 7 , wherein the control unit is configured to control the pressure at the fluidic coupling point during injection.

9. The injector of claim 7 , comprising a sample drive configured to intake the fluidic sample into the sample accommodation volume, wherein the control unit is configured to synchronize operation of the fluid drive and the sample drive for controlling the pressure.

10. The injector of claim 7 , wherein the control unit is configured to adjust a mixing ratio between the mobile phase and the fluidic sample of the sampling flow at the fluidic coupling point.

11. The injector of claim 7 , wherein the control unit is configured to adjust at least one of a predefined total pressure value and a predefined outlet flow rate value according to which the combined fluid is driven through the separation unit.

12. The injector of claim 1 , comprising a sample drive configured to intake the fluidic sample into the sample accommodation volume, wherein the fluid drive and the sample drive are controllable for injecting the combined fluid with a predefined mixing ratio between the mobile phase and the fluidic sample of the sampling flow.

13. The injector of claim 12 , configured to adjust the mixing ratio by adjusting a volume-over-time displacement characteristic by which the sample drive drives the fluidic sample of the sampling flow.

14. The injector of claim 1 , wherein the fluidic valve is switchable into a pressure adjustment switching state in which a predefined overpressure for injection is adjustable in the sample accommodation volume before switching the fluidic valve for injecting the fluidic sample towards the separation unit.

15. The injector of claim 14 , wherein the fluidic valve is switchable so that the predefined overpressure for injection triggers injection of the fluidic sample from the sample accommodation volume towards the separation unit by one of: pressure equilibration without piston motion; pressure equilibration exclusively; both pressure equilibration exclusively and without piston motion.

16. The injector of claim 1 , comprising a sample drive configured to intake an amount of fluidic sample into the sample accommodation volume and to subsequently inject multiple portions of the intaken amount of fluidic sample separately towards the separation unit, wherein the multiple portions to be injected are respectively spaced by one or more predefined delay times.

17. A sample separation apparatus, comprising:
the injector according to claim 1 ;
the fluid drive configured to drive the mobile phase; and
the separation unit configured to separate the fluidic sample in the mobile phase.

18. The sample separation apparatus of claim 17 , further comprising at least one of the following features:
a sample drive configured to intake the fluidic sample into the sample accommodation volume;
a detector configured to detect separated fractions of the fluidic sample;
a fractionating unit configured to collect separated fractions of the fluidic sample;
a degassing apparatus configured to degas the mobile phase;
the sample separation apparatus is configured as a chromatography sample separation apparatus.

19. A method for injecting a fluidic sample into a flow path between a fluid drive driving a mobile phase and a separation unit of a sample separation apparatus, the method comprising:
intaking the fluidic sample in a sample accommodation volume of an injector; and
switching a fluidic valve of the injector between a first injection switching state and a second injection switching state, wherein:
in the first injection switching state, the fluid drive, the separation unit, and the sample accommodation volume are fluidically coupled by the fluidic valve so that a sampling flow, containing at least a portion of the accommodated fluidic sample, and the mobile phase are combined at a fluidic coupling point upstream of the separation unit, thereby forming a combined fluid; and
in the second injection switching state, the sample accommodation volume is fluidically coupled by the fluidic valve between the fluid drive and the separation unit.

20. The method of claim 19 , wherein, in the second injection switching state, the fluidic sample is injected towards the separation unit driven by the fluid drive while the sample accommodation volume is located downstream of the fluid drive and upstream of the separation unit.

Assignees

• Agilent Technologies Inc

Inventors

• Thielsch Daniel
• Ortmann Thomas
• Wouters Sam

Classifications

• G01N30/20 Primary

  using a sampling valve · CPC title

• G01N30/32

  of pressure or speed (G01N30/36 takes precedence) · CPC title

• G01N2030/027

  Liquid chromatography · CPC title

• G01N2030/201

  multiport valves, i.e. having more than two ports · CPC title

• G01N2030/202

  rotary valves · CPC title