Extreme pcr
US-2015118715-A1 · Apr 30, 2015 · US
Methods for fast nucleic acid amplification
US11834708B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11834708-B2 |
| Application number | US-202117244000-A |
| Country | US |
| Kind code | B2 |
| Filing date | Apr 29, 2021 |
| Priority date | May 24, 2012 |
| Publication date | Dec 5, 2023 |
| Grant date | Dec 5, 2023 |
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- Title
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- Abstract
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Abstract
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Methods, devices, and kits are provided for performing PCR in <20 seconds per cycle, with improved efficiency and yield.
First claim
Opening claim text (preview).
That which is claimed is: 1. A kit for performing PCR on a target nucleic acid, the kit comprising a mixture of: dNTPs, a thermostable polymerase, and primers configured for amplification of the target nucleic acid sequence, wherein the polymerase to primer ratio in the kit is (0.03 to 0.4 polymerase):(total primer concentration) and the polymerase has a concentration of at least 0.5 μM. 2. The kit of claim 1 , wherein the primers have a total concentration of at least 5 μM. 3. The kit of claim 1 , wherein the polymerase is KlenTaq provided at the concentration of at least 1.0 μM. 4. The kit of claim 1 , further comprising instructions for performing PCR using a temperature cycling profile wherein each cycle is completed in a cycle time less than 20 second per cycle. 5. The kit of claim 4 , wherein the cycle time is less than 10 seconds per cycle. 6. The kit of claim 1 , wherein the target nucleic acid is eukaryotic and the kit is configured for amplifying from eukaryotic genomic DNA. 7. The kit of claim 1 , wherein the primers are a pair of primers and each primer of the pair of primers is provided at a concentration of at least 2 μM.
Assignees
Inventors
Classifications
- C12Q1/686Primary
Polymerase chain reaction [PCR] · CPC title
Water baths; Sand baths; Air baths · CPC title
with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples · CPC title
by moving sample containers · CPC title
by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip · CPC title
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Frequently asked questions
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- What does patent US11834708B2 cover?
- Methods, devices, and kits are provided for performing PCR in <20 seconds per cycle, with improved efficiency and yield.
- Who is the assignee on this patent?
- Univ Utah Res Found
- What technology area does this patent fall under?
- Primary CPC classification C12Q1/686. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Dec 05 2023 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 1 related publication on this page (citations in our corpus or others sharing the same primary CPC).