Respirator, measurement device and measuring method for measuring respiratory rate of biofilm

Patent metadata

Field	Value
Publication number	US-11834645-B2
Application number	US-201816631462-A
Country	US
Kind code	B2
Filing date	Sep 7, 2018
Priority date	Aug 28, 2018
Publication date	Dec 5, 2023
Grant date	Dec 5, 2023

Abstract

A respirator for measuring a respiratory rate of a biofilm includes a body, a carbon dioxide absorption tube and a perforated partition disposed in an interior of the body. The perforated partition divides the interior of the body into an oxygen mass-transfer stir zone including a stirring device and a biofilm reaction zone for placing MBBR fillers. The carbon dioxide absorption tube includes an air vent and contains a solution capable of absorbing CO 2 . The perforated partition has a first hole corresponding to a middle portion and a lower portion of the perforated partition and acting as a liquid-exchange channel, and a second hole corresponding to an upper portion of the perforated partition and acting as a gas-exchange channel, and the oxygen mass-transfer stir zone is communicated with the biofilm reaction zone through the liquid-exchange channel and the gas-exchange channel.

First claim

What is claimed is:

1. A respirator for measuring a respiratory rate of a biofilm, characterized in that, the respirator comprises a body ( 1 ), a carbon dioxide absorption tube ( 11 ) disposed in an interior of the body and at least one perforated partition ( 6 ) disposed in an interior of the body ( 1 );
the at least one perforated partition ( 6 ) divides the interior of the body ( 1 ) into an oxygen mass-transfer stir zone ( 8 ) and at least one biofilm reaction zone ( 3 ), the oxygen mass-transfer stir zone ( 8 ) is provided with a stirring device ( 7 ), the at least one biofilm reaction zone ( 3 ) is used for placing MBBR fillers ( 2 );
the carbon dioxide absorption tube ( 11 ) is provided with an air vent ( 10 ) and contains a solution, configured to absorb CO 2 within the body ( 1 );
the at least one perforated partition ( 6 ) has a plurality of first holes and a second hole, the plurality of first holes is located at a middle portion and a lower portion of the at least one perforated partition ( 6 ) and defining a plurality of liquid-exchange channels ( 5 ), the second hole is located at an upper portion of the at least one perforated partition ( 6 ) and defining a gas-exchange channel ( 12 ), and the oxygen mass-transfer stir zone ( 8 ) is in communication with the at least one biofilm reaction zone ( 3 ) through the plurality of liquid-exchange channels ( 5 ) and the gas-exchange channel ( 12 );
a first opening and at least one second opening provided on the body, the first opening corresponds to the oxygen mass-transfer stir zone ( 8 ), the at least one second opening corresponds to the at least one biofilm reaction zone ( 3 ); and
a headspace cap ( 9 ) coupled to the first opening and configured to seal the first opening, and at least one sealing cap ( 13 ) coupled to the at least one second opening and configured to seal the at least one second opening, and
wherein the carbon dioxide absorption tube ( 11 ) is coupled to the headspace cap ( 9 ).

2. The respirator according to claim 1 , wherein the at least one perforated partition ( 6 ) comprises two perforated partitions ( 6 ), wherein the at least one biofilm reaction zone includes two biofilm reaction zones, between the two perforated partitions ( 6 ) is the oxygen mass-transfer stir zone ( 8 ), and between the perforated partition ( 6 ) and the body ( 1 ) is the two biofilm reaction zones ( 3 ).

3. The respirator according to claim 2 , wherein the at least one second opening includes two second openings, wherein the at least one sealing cap includes two sealing caps, the two second openings correspond to the two biofilm reaction zones ( 3 ) respectively, and the two second openings are each provided with a sealing cap ( 13 ) of the two sealing caps for sealing the two second openings respectively.

4. The respirator according to claim 1 , wherein a bottom of the at least one biofilm reaction zone ( 3 ) is further provided with a corner sloping board ( 4 ) at a corner of the body ( 1 ).

5. The respirator according to claim 1 , wherein the body ( 1 ) is cuboid or cylindrical.

6. The respirator according to claim 1 , wherein the plurality of liquid-exchange channels ( 5 ) is evenly distributed at the middle portion and the lower portion of the at least one perforated partition ( 6 ).

7. The respirator according to claim 1 , wherein the stirring device ( 7 ) is a magnetic stirrer located at a bottom of the oxygen mass-transfer stir zone ( 8 ).

8. A measuring device for a respiratory rate of a biofilm, characterized in that, the measuring device comprises a respirometer for measuring an oxygen-absorption rate and the respirator according to claim 1 , and the respirometer is communicated with an interior of the respirator.

9. A measuring method for a respiratory rate of a biofilm, characterized in that, comprising the following steps:
providing the measuring device according to claim 8 ;
putting the MBBR fillers ( 2 ) attached with the biofilm into the at least one biofilm reaction zone ( 3 ), adding a water sample to immerse the MBBR fillers ( 2 ) in the at least one biofilm reaction zone ( 3 );
adding the solution capable of absorbing CO 2 to the carbon dioxide absorption tube ( 11 ), sealing the at least one biofilm reaction zone ( 3 ) and the oxygen mass-transfer stir zone ( 8 ), connecting the respirometer to the interior of the body ( 1 ) via a tube, activating the stirring device ( 7 ) located in the oxygen mass-transfer stir zone ( 8 ); and
acquiring an inspiratory rate and a total inspiratory amount recorded by the respirometer.

10. A respirator for measuring a respiratory rate of a biofilm, characterized in that, the respirator comprises a body ( 1 ), a carbon dioxide absorption tube ( 11 ) disposed in an interior of the body and a perforated partition ( 6 ) disposed in an interior of the body ( 1 );
a perforated partition ( 6 ) divides the interior of the body ( 1 ) into an oxygen mass-transfer stir zone ( 8 ) and a biofilm reaction zone ( 3 ), the oxygen mass-transfer stir zone ( 8 ) is provided with a stirring device ( 7 ), the biofilm reaction zone ( 3 ) is used for placing MBBR fillers ( 2 );
the carbon dioxide absorption tube ( 11 ) is provided with an air vent ( 10 ) and contains a solution configured to absorb CO 2 within the body ( 1 );
the perforated partition ( 6 ) has a plurality of first holes and a second hole, the plurality of first holes is located at a middle portion and a lower portion of the perforated partition ( 6 ) and defining a plurality of liquid-exchange channels ( 5 ), the second hole is located at an upper portion of the perforated partition ( 6 ) and defining a gas-exchange channel ( 12 ), and the oxygen mass-transfer stir zone ( 8 ) is in communication with the biofilm reaction zone ( 3 ) through the a plurality of liquid-exchange channels ( 5 ) and the gas-exchange channel ( 12 );
a first opening and a second opening provided on the body, the first opening corresponds to the oxygen mass-transfer stir zone ( 8 ), the second opening corresponds to the biofilm reaction zone ( 3 ); and
a headspace cap ( 9 ) coupled to the first opening and configured to seal the first opening, and sealing cap ( 13 ) coupled to the second opening and configured to seal the second opening, and
wherein the carbon dioxide absorption tube ( 11 ) is coupled to the headspace cap ( 9 ).

11. The respirator according to claim 10 , wherein the perforated partition ( 6 ) is a cylindrical perforated partition ( 6 ) and located at a middle portion of the body ( 1 ), an interior of the cylindrical perforated partition ( 6 ) defines the oxygen mass-transfer stir zone ( 8 ), and between an exterior of the cylindrical perforated partition ( 6 ) and the body ( 1 ) defines the biofilm reaction zone ( 3 ).

12. The respirator according to claim 10 , wherein a bottom of the biofilm reaction zone ( 3 ) is further provided with a corner sloping board ( 4 ) at a corner of the body ( 1 ).

13. The respirator according to claim 10 , wherein the body ( 1 ) is cuboid or cylindrical.

14. The respirator according to claim 10 , wherein the first hole comprises a plurality of first holes, and the plurality of liquid-exchange channels ( 5 ) is evenly distributed at the middle portion and the lower portion of the perforated partition ( 6 ).

15. The respirator according to claim 10 , wherein the stirring device ( 7 ) is a magnetic stirrer located at a bottom of the oxygen mass-transfer stir zone ( 8 ).

16. A measuring device for a respiratory rate of a biofilm, characterized in that, the measuring device comprises a respirometer for measuring an oxy

Assignees

• Univ Jinan

Inventors

• Liu Guoqiang
• Lu Zichuan

Classifications

• C12M41/46 Primary

  of cellular or enzymatic activity or functionality, e.g. cell viability · CPC title

• C12M23/34

  Internal compartments or partitions · CPC title

• C12M23/38

  Caps; Covers; Plugs; Pouring means · CPC title

• C12M27/04

  with introduction of gas through the stirrer or mixing element · CPC title

• C12M29/20

  Degassing; Venting; Bubble traps (means for collection or storage of gas C12M23/36; gas collection apparatus for laboratory use B01L5/02) · CPC title