Vitamin B2 detection by mass spectrometry

Patent metadata

Field	Value
Publication number	US-11821889-B2
Application number	US-202217715202-A
Country	US
Kind code	B2
Filing date	Apr 7, 2022
Priority date	Dec 18, 2008
Publication date	Nov 21, 2023
Grant date	Nov 21, 2023

Abstract

Methods are described for measuring the amount of a vitamin B2 in a sample. More specifically, mass spectrometric methods are described for detecting and quantifying vitamin B2 in a sample utilizing on-line extraction methods coupled with tandem mass spectrometric techniques.

First claim

That which is claimed is:

1. A method for determining the amount of vitamin B2 in a biological sample from a human, said method comprising:
(a) purifying the sample by turbulent flow liquid chromatography and high performance liquid chromatography (HPLC);
(b) ionizing vitamin B2 to produce one or more ions detectable by tandem mass spectrometry;
(c) determining by mass spectrometry the amount of a fragment ion with a mass to charge ratio of 243.2±0.5; and
(d) determining the amount of vitamin B2 in the sample from the amount of the fragment ion in step (c).

2. The method of claim 1 , wherein said HPLC and mass spectrometry are conducted with on-line processing.

3. The method of claim 1 , wherein said biological sample comprises plasma or serum.

4. The method of claim 1 , wherein ionizing consists of electrospray ionization (ESI).

5. The method of claim 1 , wherein ESI is in positive ion mode.

6. The method of claim 1 , wherein the HPLC is a reverse-phase high performance liquid chromatography (RP-HPLC).

7. The method of claim 1 , further comprising protein precipitation.

8. The method of claim 7 , wherein an acid is used for precipitating protein.

Assignees

• Quest Diagnostics Invest Inc

Inventors

• Yang Changming
• Chan Sum

Classifications

• G01N33/487 Primary

  of liquid biological material · CPC title

• G01N30/72

  Mass spectrometers {(mass spectrometers per se H01J49/00)} · CPC title

• G01N30/7233

  interfaced to liquid or supercritical fluid chromatograph (interfaces in general for introducing or extracting samples to be analysed with specially adapted mass spectrometer, see H01J49/04) · CPC title

• G01N33/6848

  Methods of protein analysis involving mass spectrometry · CPC title

• G01N33/82

  involving vitamins {or their receptors} · CPC title