Brachiaria endophytes and related methods

Patent metadata

Field	Value
Publication number	US-11818994-B2
Application number	US-202117518882-A
Country	US
Kind code	B2
Filing date	Nov 4, 2021
Priority date	Sep 24, 2015
Publication date	Nov 21, 2023
Grant date	Nov 21, 2023

Abstract

The present invention relates to endophytes, in particular endophytes associated with plants of the Brachiaria - Urochloa species complex, plants infected with the endophytes, products produced by the endophytes, and related methods.

First claim

The invention claimed is:

1. A plant of Brachiaria - Urochloa species complex inoculated with one or more isolated endophytes selected from the group consisting of Hypocrea sp./ Acremonium sp. 2.15.A.2, Acremonium sp. 2.3.C.1 , Microsphaeropsis arundis 2.10.D.1 , Sarocladium sp./ Acremonium sp. 2.12.B.1 , Sarocladium sp./ Acremonium sp. 2.10.C.2 and Sarocladium sp./ Acremonium sp. 2.11.B.1, as deposited at the National Measurement Institute with accession numbers V15/028237, V15/028238, V15/028239, V15/028240, V15/028241 and V15/028242, respectively, and wherein said plant of Brachiaria - Urochloa species complex comprising an endophyte-free host plant is stably infected with said one or more isolated endophyte, and wherein said plant of Brachiaria - Urochloa species complex is not naturally associated with said one or more isolated endophyte.

2. The inoculated plant of Brachiaria - Urochloa species complex according to claim 1 , wherein said inoculated plant of Brachiaria - Urochloa species complex has beneficial properties when compared with an uninoculated control plant of the same Brachiaria - Urochloa species complex, and wherein said beneficial properties are improved tolerance to water and/or nutrient stress or improved resistance to pests and/or diseases.

3. The inoculated plant of Brachiaria - Urochloa species complex according to claim 1 , said inoculated plant of Brachiaria - Urochloa species complex has beneficial properties when compared with an uninoculated control plant of the same Brachiaria - Urochloa species complex, and wherein, said beneficial properties is the production of a fusicoccane compound.

4. The inoculated plant of Brachiaria - Urochloa species complex according to claim 3 , wherein the fusicoccane compound is brachialactone.

5. The inoculated plant of Brachiaria - Urochloa species complex according to claim 1 , wherein said plant of Brachiaria - Urochloa species complex is selected from the group consisting of Brachiaria brizantha, Brachiaria decumbens, Brachiaria humidicola, Brachiaria stolonifera, Brachiaria ruziziensis, Brachiaria dictyoneura, Urochloa brizantha, Urochloa decumbens, Urochloa humidicola, Urochloa mosambicensis, Brachiaria marlothii, Brachiaria nigropedata, Urochloa dictyoneura, Urochloa oligotricha, Urochloa panicoides, Brachiaria obtusiflora, Brachiaria serrifolia, Urochloa advena, Urochloa arrecta, Urochloa brachyura, Urochloa eminii, Urochloa mollis, Urochloa xantholeuca, Urochloa oliqotricha, Urochloa panicoides, Urochloa plantaqima, Urochloa platynota, Urochloa xantholeuca, Brachiaria holosericea, Brachiaria reptans, Brachiaria milliformi and Brachiaria distachya.

6. A method of infecting a plant of Brachiaria - Urochloa species complex, comprising inoculating said plant of Brachiaria - Urochloa species complex with one or more isolated endophytes selected from the group consisting of Hypocrea sp./ Acremonium sp. 2.15.A.2, Acremonium sp. 2.3.C.1, Microsphaeropsis arundis 2.10.D.1, Sarocladium sp./ Acremonium sp. 2.12.B.1, Sarocladium sp./ Acremonium sp. 2.10.C.2 and Sarocladium sp./ Acremonium sp. 2.11.B.1, as deposited at the National Measurement Institute with accession numbers V15/028237, V15/028238, V15/028239, V15/028240, V15/028241 and V15/028242, respectively, and wherein said plant of Brachiaria - Urochloa species complex comprising an endophyte-free host plant is stably infected with said one or more isolated endophyte, and wherein said plant of Brachiaria - Urochloa species complex is not naturally associated with said one or more isolated endophyte.

7. The method of claim 6 , wherein said inoculated plant of Brachiaria - Urochloa species complex has beneficial properties when compared with an uninoculated control plant of the same Brachiaria - Urochloa species complex, and wherein said beneficial properties are improved tolerance to water and/or nutrient stress or improved resistance to pests and/or diseases.

8. The method of claim 6 , wherein said inoculated plant of Brachiaria - Urochloa species complex has beneficial properties when compared with an uninoculated control plant of the same Brachiaria - Urochloa species complex, and wherein said beneficial properties is the production of a fusicoccane compound.

9. The method of claim 8 , wherein the fusicoccane compound is brachialactone.

10. The method of claim 6 , wherein said plant of Brachiaria - Urochloa species complex is selected from the group consisting of Brachiaria brizantha, Brachiaria decumbens, Brachiaria humidicola, Brachiaria stolonifera, Brachiaria ruziziensis, Brachiaria dictyoneura, Urochloa brizantha, Urochloa decumbens, Urochloa humidicola, Urochloa mosambicensis, Brachiaria marlothii, Brachiaria nigropedata, Urochloa dictyoneura, Urochloa oligotricha, Urochloa panicoides, Brachiaria obtusiflora, Brachiaria serrifolia, Urochloa advena, Urochloa arrecta, Urochloa brachyura, Urochloa eminii Urochloa mollis, Urochloa xantholeuca, Urochloa oligotricha, Urochloa panicoides, Urochloa plantagima, Urochloa platynota, Urochloa xantholeuca, Brachiaria holosericea, Brachiaria reptans, Brachiaria milliformi and Brachiaria distachya.

Assignees

• Agriculture Victoria Serv Pty

Inventors

• Spangenberg German Carlos
• Guthridge Kathryn Michaela
• Mann Ross
• Sawbridge Timothy Ivor
• Hettiarachchige Inoka Kumari
• Ekanayake Piyumi Niroshini
• Brohier Natasha Denise
• Rochfort Simone Jane
• Edwards Jacqueline

Classifications

• A01H17/00 Primary

  Symbiotic or parasitic combinations including one or more new plants, e.g. mycorrhiza (lichens A01H15/00) · CPC title

• A01N63/30

  Microbial fungi; Substances produced thereby or obtained therefrom · CPC title

• C07D493/00

  Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system · CPC title

• C07D493/08

  Bridged systems · CPC title

• C12N1/14

  Fungi (culture of mushrooms A01G18/00; as new plants A01H15/00); Culture media therefor · CPC title