Method for identifying molecular structure

Patent metadata

Field	Value
Publication number	US-11815475-B2
Application number	US-201816490710-A
Country	US
Kind code	B2
Filing date	Feb 28, 2018
Priority date	Mar 1, 2017
Publication date	Nov 14, 2023
Grant date	Nov 14, 2023

Abstract

The present invention provides a novel method for identifying a molecular structure by a single crystal X-ray analysis. A single crystal that gives an X-ray diffraction spectrum sufficient for determining a structure of a molecule can be efficiently obtained by including a test molecule in a metal complex, and then crystallizing the test-molecule included in the metal complex. By analyzing this single crystal by an X-ray analysis, it is possible to determine a structure of the test molecule without obtaining a single crystal of the test molecule. With the novel method according to the present invention, the structure of a test molecule in a trace amount of a sample can also be determined.

First claim

The invention claimed is:

1. A method for a structural analysis of a test molecule with an X-ray or a neutron beam, the method comprising the steps of:
(A) mixing together a sample containing the test molecule and a metal complex capable of including the test molecule to generate a test molecule-metal complex inclusion body;
(B) dispensing an inclusion solution containing the test molecule-metal complex inclusion body to prepare a plurality of independent crystallization solutions at least including a first crystallization solution and a second crystallization solution;
(C) generating a plurality of crystals of the test molecule-metal complex inclusion body in the plurality of independent crystallization solutions under similar or different crystallization conditions;
(D) screening the plurality of crystals of the test molecule-metal complex inclusion body generated in the step (C) to select a targeted crystal;
(E) irradiating the targeted crystal with an X-ray or a neutron beam to acquire diffraction data; and
(F) analyzing the diffraction data to determine a structure of the test molecule.

2. The method according to claim 1 , wherein volumes of the first crystallization solution and the second crystallization solution are each 100 nL to 10 μL.

3. The method according to claim 1 , wherein volumes of the first crystallization solution and the second crystallization solution are each 200 nL to 2 μL.

4. The method according to claim 1 , wherein a weight of the test molecule contained in the sample in the step (A) is 1 ng to 1 mg.

5. The method according to claim 4 , wherein the weight of the test molecule contained in the sample in the step (A) is 5 ng to 1 mg.

6. The method according to claim 1 , wherein the step (A) comprises mixing the sample containing the test molecule, an insertion molecule, and a metal complex capable of including the test molecule together to generate a test molecule/insertion molecule-metal complex inclusion body.

7. The method according to claim 6 , wherein the step (A) further comprises using a change in a color of a solution containing a mixture of the test molecule, the insertion molecule, and the metal complex as an index of a generation of the test molecule/insertion molecule-metal complex inclusion body.

8. The method according to claim 1 , wherein the step (C) is automatically performed.

9. The method according to claim 1 , further comprising the step of contacting a metal molecule with a ligand to prepare the metal complex.

10. The method according to claim 1 , further comprising adjusting an evaporation rate of the plurality of independent crystallization solutions in the step (B).

11. The method according to claim 10 , wherein the adjusting is performed through a vapor transport method.

12. The method according to claim 1 , wherein the metal complex has a hydrophilic moiety.

13. The method according to claim 1 , wherein the test molecule has a hydrophilic moiety.

14. The method according to claim 1 , wherein a solvent of each of the plurality of independent crystallization solutions is an aqueous solvent.

15. The method according to claim 1 , wherein at least a partial structure of a structure of the test molecule is unknown or uncertain, and at least a partial structure in the at least partial structure of the structure of the test molecule is determined.

16. A method for using a kit for a structural analysis of a test molecule with an X-ray or a neutron beam, comprising:
providing the kit comprising:
a metal complex capable of including the test molecule; and
a crystallization solvent; and

carrying out steps for the structural analysis of the test molecule with the X-ray or the neutron beam, the steps for the structural analysis comprising:
(A) mixing together a sample containing the test molecule, the metal complex capable of including the test molecule, and the crystallization solvent to generate a test molecule-metal complex inclusion body;
(B) dispensing an inclusion solution containing the test molecule-metal complex inclusion body to prepare a plurality of independent crystallization solutions at least including a first crystallization solution and a second crystallization solution;
(C) generating a plurality of crystals of the test molecule-metal complex inclusion body in the plurality of independent crystallization solutions under similar or different crystallization conditions;
(D) screening the plurality of crystals of the test molecule-metal complex inclusion body generated in the step (C) to select a targeted crystal;
(E) irradiating the targeted crystal with an X-ray or a neutron beam to acquire diffraction data; and
(F) analyzing the diffraction data to determine a structure of the test molecule.

17. A kit for a structural analysis of a test molecule with an X-ray or a neutron beam, the kit comprising:
a metal complex capable of including the test molecule,
a crystallization solvent used to crystallize the metal complex including the test molecule, and
at least one insertion molecule.

18. The kit according to claim 17 , further comprising at least two insertion molecules.

19. A kit for a structural analysis of a test molecule with an X-ray or a neutron beam, the kit comprising:
a metal complex capable of including the test molecule, and
at least two crystallization solvents used to crystallize the metal complex including the test molecule,
the first crystallization solvent containing the metal complex dissolved therein, and the second crystallization solvent containing the metal complex in concentration different from that in the first crystallization solvent.

20. A kit for a structural analysis of a test molecule with an X-ray or a neutron beam, the kit comprising:
at least two metal complexes capable of including the test molecule,
wherein each of the at least two metal complexes has a hydrophilic moiety, and
a crystallization solvent used to crystallize the at least two metal complexes including the test molecule.

Assignees

• Univ Tokyo

Inventors

• Fujita Makoto
• Fujita Daishi
• Domoto Yuya
• Takezawa Hiroki

Classifications

• G01N23/2055 Primary

  Analysing diffraction patterns · CPC title

• C30B7/04

  using aqueous solvents · CPC title

• C40B30/10

  by measuring physical properties, e.g. mass · CPC title

• C40B40/16

  Libraries containing metal-containing organic compounds · CPC title

• C40B50/08

  Liquid phase synthesis, i.e. wherein all library building blocks are in liquid phase or in solution during library creation; Particular methods of cleavage from the liquid support · CPC title