Production method and production apparatus for dried vitrigel membrane

Patent metadata

Field	Value
Publication number	US-11814488-B2
Application number	US-201816613254-A
Country	US
Kind code	B2
Filing date	Apr 12, 2018
Priority date	May 17, 2017
Publication date	Nov 14, 2023
Grant date	Nov 14, 2023

Abstract

A production method for a dried Vitrigel film includes, in the following order: step 1 of concentrically arranging and disposing member A having one or more recesses and member B having one or more through holes, such that the recesses overlap the through holes; step 2 of pouring a sol from the through holes of member B; step 3 of gelling the sol; step 4 of drying and vitrifying the hydrogel obtained in step 3 in a state in which it is formed in the member A and the member B; step 5 of hydrating the dried hydrogel obtained in step 4; step 6 of drying and re-vitrifying the Vitrigel obtained in step 5; and step 7 of cutting off a portion, which slightly covers the top surface of the member A, of the dried Vitrigel obtained in step 6.

First claim

The invention claimed is:

1. A production method for a dried Vitrigel film, the method comprising, in the following order:
step 1 of concentrically arranging and disposing a member A, which has one or more recesses and in which, in a bottom surface of the recess, a central part of the bottom surface is formed of a first material having low adsorptivity to a hydrogel and a peripheral part of the bottom surface is formed of a second material having high adsorptivity to a hydrogel, and a member B having one or more through holes with a cross-section of substantially equal size to a cross-section of the recess of the member A, such that the recess of the member A and the through hole of the member B overlap;
step 2 of pouring a sol from the through holes of member B;
step 3 of gelling the sol by leaving the member A and the member B in which the sol is poured to stand;
step 4 of drying and vitrifying a hydrogel obtained in step 3 in a state in which it is formed in the member A and the member B;
step 5 of hydrating the dried hydrogel obtained in step 4 in a state in which it is formed in the member A and the member B;
step 6 of drying and re-vitrifying the Vitrigel obtained in step 5 in a state in which it is formed in the member A and the member B; and
step 7 of cutting off a portion slightly covering a top surface of the member A from the dried Vitrigel obtained in step 6 using a cylindrical blade of which the cross-section is a size slightly larger than the cross-section of the recess of the member A and slightly smaller than the cross-section of the through hole of the member B.

2. The production method for a dried Vitrigel film according to claim 1 , wherein the first material is removable at the bottom surface of the recess of the member A.

Assignees

• Nat Agriculture & Food Res Org

Inventors

• Takezawa Toshiaki

Classifications

• C08J3/075 Primary

  Macromolecular gels · CPC title

• B29D7/01

  Films or sheets · CPC title

• C08J5/18

  Manufacture of films or sheets · CPC title

• C12M25/14

  Scaffolds; Matrices (in general C12N5/0068) · CPC title

• C08J2389/06

  derived from leather or skin · CPC title