Microorganisms genetically engineered to have modified N-glycosylation activity
US-9222083-B2 · Dec 29, 2015 · US
Alpha-glucose-1-phosphate synthesis from sucrose and glucan synthesis using glucan phosphorylases
US11802275B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11802275-B2 |
| Application number | US-201917261937-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jul 18, 2019 |
| Priority date | Jul 23, 2018 |
| Publication date | Oct 31, 2023 |
| Grant date | Oct 31, 2023 |
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- Title
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- Abstract
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Abstract
Official abstract text for this publication.
Reactions are disclosed herein comprising water, alpha-glucose-1-phosphate (alpha-G1P), an acceptor molecule, and an alpha-1,4-glucan phosphorylase. Novel alpha-1,4-glucan phosphorylase enzymes are also disclosed. Additional disclosures herein regard sucrose phosphorylase enzymes and methods of use thereof to produce alpha-G1P.
First claim
Opening claim text (preview).
What is claimed is: 1. An isolated composition comprising: (i) an isolated sucrose phosphorylase that comprises an amino acid sequence that is at least 90% identical to SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, or SEQ ID NO:22, and (ii) an isolated alpha-1,4-glucan phosphorylase that comprises an amino acid sequence that is at least 90% identical to SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:30, SEQ ID NO:32, SEQ ID NO:34, SEQ ID NO:36, SEQ ID NO:38, SEQ ID NO:40, SEQ ID NO:42, SEQ ID NO:44, SEQ ID NO:46, SEQ ID NO:48, SEQ ID NO:50, or SEQ ID NO:54. 2. The composition of claim 1 , wherein the sucrose phosphorylase comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, or SEQ ID NO:22. 3. The composition of claim 2 , wherein the sucrose phosphorylase comprises an amino acid sequence that is at least 97% identical to SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, or SEQ ID NO:22. 4. The composition of claim 3 , wherein the sucrose phosphorylase comprises an amino acid sequence that is at least 98% identical to SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, or SEQ ID NO:22. 5. The composition of claim 1 , wherein the composition further comprises at least water, inorganic phosphate, sucrose, and an acceptor molecule, wherein the sucrose phosphorylase produces alpha-glucose-1-phosphate in the composition, and wherein the alpha-1,4-glucan phosphorylase produces alpha-1,4-glucan in the composition. 6. The composition of claim 5 , wherein the sucrose phosphorylase comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, or SEQ ID NO:22. 7. The composition of claim 6 , wherein the sucrose phosphorylase comprises an amino acid sequence that is at least 97% identical to SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, or SEQ ID NO:22. 8. The composition of claim 7 , wherein the sucrose phosphorylase comprises an amino acid sequence that is at least 98% identical to SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, or SEQ ID NO:22. 9. The composition of claim 5 , wherein the acceptor molecule comprises a monosaccharide, disaccharide, or oligosaccharide. 10. The composition of claim 5 , wherein the acceptor molecule comprises alpha-1,4 glycosidic linkages and has a degree of polymerization (DP) of 2 to 35. 11. The composition of claim 5 , wherein the acceptor molecule comprises a polysaccharide. 12. The composition of claim 5 , wherein the alpha-1,4-glucan has at least about 90% alpha-1,4 glycosidic linkages. 13. The composition of claim 12 , wherein the alpha-1,4-glucan has at least about 99% alpha-1,4 glycosidic linkages. 14. The composition of claim 5 , wherein the alpha-1,4-glucan phosphorylase comprises an amino acid sequence that is at least 90% identical to SEQ ID NO:26, SEQ ID NO:30, SEQ ID NO:32, SEQ ID NO:34, SEQ ID NO:38, SEQ ID NO:40, SEQ ID NO:42, SEQ ID NO:44, SEQ ID NO:46, SEQ ID NO:48, or SEQ ID NO:50.
Assignees
Inventors
Classifications
- C12N9/1051Primary
Hexosyltransferases (2.4.1) · CPC title
- C12P19/04Primary
Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds · CPC title
Phosphorylase (2.4.1.1) · CPC title
Sucrose phosphorylase (2.4.1.7) · CPC title
Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title
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Frequently asked questions
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- What does patent US11802275B2 cover?
- Reactions are disclosed herein comprising water, alpha-glucose-1-phosphate (alpha-G1P), an acceptor molecule, and an alpha-1,4-glucan phosphorylase. Novel alpha-1,4-glucan phosphorylase enzymes are also disclosed. Additional disclosures herein regard sucrose phosphorylase enzymes and methods of use thereof to produce alpha-G1P.
- Who is the assignee on this patent?
- Danisco Us Inc
- What technology area does this patent fall under?
- Primary CPC classification C12N9/1051. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Oct 31 2023 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).