Microfluidic devices for optically-driven convection and displacement, kits and methods thereof

Patent metadata

Field	Value
Publication number	US-11802264-B2
Application number	US-202017067971-A
Country	US
Kind code	B2
Filing date	Oct 12, 2020
Priority date	Dec 30, 2015
Publication date	Oct 31, 2023
Grant date	Oct 31, 2023

Abstract

Apparatuses and methods are described for the use of optically driven bubble, convective and displacing fluidic flow to provide motive force in microfluidic devices. Alternative motive modalities are useful to selectively dislodge and displace micro-objects, including biological cells, from a variety of locations within the enclosure of a microfluidic device.

First claim

The invention claimed is:

1. A method of dislodging one or more micro-objects within a microfluidic device, the method comprising the steps of:
illuminating a selected discrete region containing or adjacent to one or more micro-objects disposed within a fluidic medium in an enclosure of the microfluidic device, wherein the enclosure comprises a microfluidic circuit comprising a flow region, a substrate, and at least one sequestration pen comprising: a connection region; an isolation region; and a displacement force generation region, wherein the connection region comprises a proximal opening to the flow region and a distal opening to the isolation region and wherein a fluidic connection between the isolation region and the displacement force generation region is configured to prevent passage of a micro-object from the isolation region to the displacement force generation region; and
maintaining the illumination of the selected discrete region for a first period of time sufficient to generate a dislodging force, dislodging the one or more micro-objects from a surface of the microfluidic device.

2. The method of claim 1 , wherein the step of illuminating comprises illuminating the selected discrete region with a laser.

3. The method of claim 1 , wherein the one or more micro-objects are disposed upon a surface of a substrate of the microfluidic device.

4. The method of claim 3 , wherein the one or more micro-objects are disposed and/or maintained on a surface of the substrate within the at least one sequestration pen.

5. The method of claim 1 , wherein the first period of time is in the range of about 10 microseconds to minutes, thereby creating a cavitating force dislodging the one or more micro-objects.

6. The method of claim 5 , wherein, when the one or more micro-objects is disposed and/or maintained within the at least one sequestration pen, the selected discrete region is a location distal to a proximal opening of the at least one sequestration pen to the flow region or is a central location of the at least one sequestration pen.

7. The method of claim 1 , wherein the selected discrete region is a selected point adjacent to the one or more micro-objects.

8. The method of claim 7 , wherein the step of illuminating the selected discrete region comprises directing illumination towards the substrate, microfluidic circuit material of a wall of the microfluidic device, or a thermal target.

9. The method of claim 7 , wherein when the one or more micro-objects are disposed within at least one sequestration pen, the selected discrete region comprises a selected point of microfluidic circuit material that helps define the at least one sequestration pen.

10. The method of claim 7 , wherein the step of illuminating the selected discrete region further comprises heating a first portion of the fluidic medium located within or adjacent to the selected discrete region, thereby creating a cavitating force.

11. The method of claim 7 , wherein the method further comprises:
heating a first portion of the fluidic medium; and,
creating a persistent gaseous bubble displacing a second portion of the fluidic medium surrounding the one or more micro-objects.

12. The method of claim 7 , wherein the method further comprises:
heating a first portion of the fluidic medium; and
creating one or more gaseous bubbles, thereby creating a shear flow of the fluidic medium towards the one or more micro-objects.

13. The method of claim 7 , wherein the method further comprises:
heating a first portion of the fluidic medium;
creating a plurality of gaseous bubbles configured to stream towards the one or more micro-objects; and
contacting the one or more micro-objects with a meniscus of at least one gaseous bubble of the plurality of gaseous bubble.

14. The method of claim 7 , wherein, when the one or more micro-objects are maintained within at least one sequestration pen, the selected discrete region comprises at least a part of a wall forming a distal end of the at least one sequestration pen, wherein the wall is positioned opposite to a proximal opening to the flow region.

15. The method of claim 7 , wherein when the one or more micro-objects are disposed within at least one sequestration pen, the selected discrete region is located in a displacement force generation region of the at least one sequestration pen.

16. The method of claim 15 , wherein the one or more micro-objects are disposed within an isolation region of the at least one sequestration pen and the displacement force generation region is fluidically connected to the isolation region.

17. The method of claim 1 , further comprising a step of exporting the one or more micro-objects from at least one sequestration pen disposed within the enclosure.

18. The method of claim 17 , wherein the step of exporting the one or more micro-objects from the at least one sequestration pen comprises moving the one or more micro-objects with di electrophoresis force.

19. The method of claim 1 , further comprising a step of exporting the one or more micro-objects from the flow region of the enclosure of the microfluidic device.

20. The method of claim 19 , wherein the step of exporting the one or more micro-objects from the flow region comprises using fluidic flow or dielectrophoresis forces.

Assignees

• Berkeley Lights Inc
• Phenomex Inc

Inventors

• Kurz Volker L S
• Lionberger Troy A
• Sackmann Eric K
• Szeto Kai W
• Lebel Paul M
• Bruhn Brandon R
• Breinlinger Keith J
• Hobbs Eric D
• Mcfarland Andrew W
• Nevill J Tanner
• Wang Xiaohua

Classifications

• C12M23/16 Primary

  Microfluidic devices; Capillary tubes (integrated microfluidic structures B01L3/5027; microreactors B01J19/0093) · CPC title

• B01L3/502761 Primary

  specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules · CPC title

• C12M23/20

  Material Coatings (immunocoatings C12M25/00) · CPC title

• C12M33/12

  by pressure · CPC title

• B01L2200/0668

  Trapping microscopic beads · CPC title