Method for preparing crude polysaccharide based on fermentation of corn stover and dried bean curd residue by Cordyceps militaris

Patent metadata

Field	Value
Publication number	US-11795484-B2
Application number	US-202017059395-A
Country	US
Kind code	B2
Filing date	Apr 29, 2020
Priority date	Apr 29, 2019
Publication date	Oct 24, 2023
Grant date	Oct 24, 2023

Abstract

The application discloses a method for preparing crude polysaccharides based on synergistic fermentation with corn stover and fungus, including the following steps: step (1): drying corn stover, pulverizing, placing in a hydrothermal reactor, adding deionized water, sealing the hydrothermal reactor, placing in a heating device, reacting, taking out reaction products, centrifuging and drying a solid; step (2): adding the products obtained in step (1) and dried bean curd residue to a container, adding distilled water, mixing uniformly, sterilizing to obtain a mixed material, inoculating a fungus inoculum under aseptic conditions, sealing the container and cultivating in the dark to obtain crude polysaccharides. The disclosure uses corn stover and bean curd residue as raw materials to achieve efficient utilization of discarded biomass, reduce environmental pollution, and improve utilization of corn stover.

First claim

What is claimed is:

1. A method for preparing crude polysaccharides based on fermentation of corn stover and dried bean curd residue by Cordyceps militaris , comprising the following steps:
step (1): drying corn stover, pulverizing, screening through a 40-mesh sieve, placing in a hydrothermal reactor, adding deionized water 10-12 times the mass of the corn stover, sealing the hydrothermal reactor, placing in a heating device, heating to 170-190° C. to allow reaction for 10-30 min, taking out the hydrothermal reactor, cooling to room temperature, taking out reaction products, centrifuging and drying a solid to obtain hydrothermal reaction products;
step (2): based on mass, adding 1-2 parts of the hydrothermal reaction products obtained in step (1) and 1-3 parts by mass of dried bean curd residue to a container, adding distilled water to adjust a water content to 65%-75%, mixing uniformly, sterilizing to obtain a mixed material, inoculating Cordyceps militaris inoculum to the mixed material under aseptic conditions, sealing the container and placing in a fermentation incubator at 23° C. in the dark for 30-35 d to obtain crude polysaccharides.

2. The method according to claim 1 , wherein a ratio of the mixed material to the Cordyceps militaris inoculum is 100 mg:10 ml.

3. The method according to claim 1 , wherein the Cordyceps militaris inoculum is prepared by a method comprising the following steps:
step (A): inoculating Cordyceps militaris on a potato dextrose agar (PDA) solid medium under aseptic conditions, placing in an incubator at 22-25° C. for 7-9 d to obtain a culture, inoculating the culture on the PDA solid medium and placing in an incubator at 22-25° C. for 7-9 d to obtain a fungus mass;
step (B): inoculating the fungus mass obtained in the step (A) into a first liquid culture medium, placing in a thermostatic culture shaker, and cultivating to mature at 20° C. and 150 r/min in the dark to obtain the Cordyceps militaris inoculum;
wherein the first liquid culture medium has a composition as follows: 30 g of soluble starch, 10 g of glucose, 10 g of peptone, 2 g of potassium dihydrogen phosphate, 2 g of magnesium sulfate, and 10 mg of vitamin B 1, added with water to 1,000 mL, pH=6.5.

4. The method according to claim 3 , wherein the PDA solid medium comprises 3.0 g/L of KH 2 PO 4 , 20.0 g/L of glucose, 1.5 g/L of MgSO 4 7H 2 O, 20.0 g/L of agar, 4.0 g/L of potato extract, and water as balance.

5. The method according to claim 2 , wherein the Cordyceps militaris inoculum is prepared by a method comprising the following steps:
step (A): inoculating Cordyceps militaris on a potato dextrose agar (PDA) solid medium under aseptic conditions, placing in an incubator at 22-25° C. for 7-9 d to obtain a culture, inoculating the culture on the PDA solid medium and placing in an incubator at 22-25° C. for 7-9 d to obtain a fungus mass;
step (B): inoculating the fungus mass obtained in the step (A) into a first liquid culture medium, placing in a thermostatic culture shaker, and cultivating to mature at 20° C. and 150 r/min in the dark to obtain the Cordyceps militaris inoculum;
wherein the first liquid culture medium has a composition as follows: 30 g of soluble starch, 10 g of glucose, 10 g of peptone, 2 g of potassium dihydrogen phosphate, 2 g of magnesium sulfate, and 10 mg of vitamin B 1, added with water to 1,000 mL, pH=6.5.

6. The method according to claim 2 , wherein the PDA solid medium comprises 3.0 g/L of KH 2 PO 4 , 20.0 g/L of glucose, 1.5 g/L of MgSO 4 7H 2 O, 20.0 g/L of agar, 4.0 g/L of potato extract, and water as balance.

Assignees

• Univ Tianjin

Inventors

• Song Yingjin
• Chen Guanyi
• Zhong Lei
• Zhang yingxiu
• Lin Fawei
• Wang Caiyu
• Meng Shuyan

Classifications

• C12P19/04 Primary

  Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds · CPC title

• C12R2001/645

  Fungi {; Processes using fungi} · CPC title

• C12N1/14

  Fungi (culture of mushrooms A01G18/00; as new plants A01H15/00); Culture media therefor · CPC title