Livestock animals with improved growth performance

Patent metadata

Field	Value
Publication number	US-11793179-B2
Application number	US-202117248071-A
Country	US
Kind code	B2
Filing date	Jan 7, 2021
Priority date	Jan 7, 2020
Publication date	Oct 24, 2023
Grant date	Oct 24, 2023

Abstract

Livestock animals and progeny thereof comprising at least one edited chromosomal sequence that alters expression or activity of a somatostatin receptor (SSTR) protein are provided. Livestock animal cells that contain such edited chromosomal sequences are also provided. The livestock animals have improved growth performance and weight gain. Methods for producing livestock animals with increased growth performance are also provided.

First claim

What is claimed is:

1. A genetically edited male or female pig, or progeny thereof, comprising an edited chromosomal sequence that reduces or eliminates expression or activity of a somatostatin receptor 2 (SSTR2) protein, wherein growth performance of the male pig comprising the edited chromosomal sequence is improved when compared to a non-edited male pig.

2. The genetically edited male or female pig of claim 1 , wherein the edited chromosomal sequence comprises a substitution, insertion, or deletion of one or more nucleotides in the SSTR2 coding sequence.

3. The genetically edited male or female pig of claim 1 , wherein the edited chromosomal sequence eliminates the expression or activity of the SSTR2 protein.

4. The genetically edited male or female pig of claim 1 , wherein the edited chromosomal sequence comprises no exogenously introduced sequence.

5. The genetically edited male or female pig of claim 1 , wherein the pig further comprises an edited chromosomal sequence that reduces or eliminates expression or activity of a SSTR1, SSTR3, SSTR4, or SSTR5 protein.

6. The genetically edited male or female pig of claim 1 , wherein the pig is heterozygous or homozygous for the edited chromosomal sequence.

7. The genetically edited male or female pig of claim 1 , wherein the pig is a male pig.

8. The genetically edited male or female pig of claim 1 , wherein the edited chromosomal sequence comprises SEQ ID NO: 3 or 4.

9. A cell of the genetically edited male or female pig of claim 1 .

10. The cell of claim 9 , wherein the edited chromosomal sequence comprises a substitution, insertion, or deletion of one or more nucleotides in the SSTR2 coding sequence.

11. The cell of claim 9 , wherein the cell further comprises an edited chromosomal sequence that reduces or eliminates expression or activity of a SSTR1, SSTR3, SSTR4, or SSTR5 protein.

12. The cell of claim 9 , wherein the cell is heterozygous or homozygous for the edited chromosomal sequence.

13. The cell of claim 9 , further comprising a conditional knock-out system for conditional expression of the SSTR2 protein.

14. The cell of claim 9 , wherein the cell is a sperm cell or an egg cell.

15. The cell of claim 9 , wherein the cell is a somatic cell.

16. The cell of claim 9 , wherein the edited chromosomal sequence comprises SEQ ID NO: 3 or 4.

17. A method of generating a male or female pig comprising:
editing a chromosomal sequence of the male or female pig to create a modification which reduces or eliminates the expression or activity of a somatostatin receptor 2 (SSTR2) protein, wherein growth performance of the male pig comprising the edited chromosomal sequence is improved.

18. The method of claim 17 , wherein the editing is by use of a TALEN, a zinc finger nuclease, or a CRISPR system.

19. The method of claim 17 , wherein the generating comprises use of somatic cell nuclear transfer.

20. The method of claim 17 , wherein the edited chromosomal sequence comprises a substitution, insertion, or deletion of one or more nucleotides in the SSTR2 coding sequence.

Assignees

• Univ Iowa State Res Found Inc

Inventors

• Ross Jason Wayne
• Schultz Ronald Blythe
• Gabler Nicholas Kurt

Classifications

• A01K67/0276 Primary

  Knock-out vertebrates · CPC title

• C12N15/907

  in mammalian cells · CPC title

• A01K2217/075

  inducing loss of function, i.e. knock out · CPC title

• A01K2217/203

  Animal model comprising inducible/conditional expression system, e.g. hormones, tet · CPC title

• A01K2227/108

  Swine · CPC title