System and method for sperm sorting

Patent metadata

Field	Value
Publication number	US-11788943-B2
Application number	US-202217872872-A
Country	US
Kind code	B2
Filing date	Jul 25, 2022
Priority date	Nov 20, 2013
Publication date	Oct 17, 2023
Grant date	Oct 17, 2023

Abstract

A system and method for sorting sperm is provided. The system includes a housing and a microfluidic system supported by the housing. The system also includes an inlet providing access to the microfluidic system to deliver sperm to the microfluidic system and an outlet providing access to the microfluidic system to harvest sorted sperm from the microfluidic system. The microfluidic system provides a flow path for sperm from the inlet to the outlet and includes at least one channel extending from the inlet to the outlet to allow sperm delivered to the microfluidic system through the inlet to progress along the flow path toward the outlet. The microfluidic system also includes a filter including a first plurality of micropores arranged in the flow path between the inlet and the outlet to cause sperm traveling along the flow path to move against through the filter and gravity to reach the outlet.

First claim

The invention claimed is:

1. A system for sorting sperm of a sample, comprising:
a housing comprising a lower component and an upper component coupled together;
a fluidic system supported by the housing;
an inlet extending through the upper component;
a collection chamber formed by the lower component and the upper component when coupled together, and configured to pass motile sperm for harvesting and restrict non-motile sperm, the collection chamber including a first chamber and a second chamber positioned above the first chamber;
at least one straight channel directly connecting the inlet to the first chamber;
a horizontally-positioned filter including a plurality of 1 um to 10 um diameter micropores and arranged in the collection chamber to cause sperm traveling along a flow path to move from the first chamber through the filter and against gravity to reach the second chamber; and
an outlet extending through the upper component and connected to the second chamber, the outlet sized and configured to facilitate harvesting functional sperm out of the system, wherein the functional sperm are suitable for fertilization and are at least some of the motile sperm, wherein the fluidic system is configured such that upon use, a percentage of motile sperm to non-motile sperm harvested through the outlet is greater than the percentage in the sample.

2. The system of claim 1 , wherein the plurality of micropores are at least 3 um and less than 10 um in diameter.

3. The system of claim 1 , wherein the fluidic system is configured such that upon use the motile sperm pass through the filter into the second chamber and traverse a path from the second chamber into the outlet in a flow-free manner.

4. The system of claim 3 , wherein the outlet does not comprise a filter.

5. The system of claim 3 , wherein a top portion of the second chamber of the collection chamber comprises an opening that provides access to the fluidic system, in addition to the outlet.

6. The system of claim 1 , wherein the outlet is an open end of an additional collection chamber that is connected to the second chamber by a fluid connection channel.

7. The system of claim 2 , wherein the outlet does not comprise a filter connected to another outlet.

8. The system of claim 1 , further comprising a slide supporting the housing, wherein the slide is located under the lower component and forms a lower surface of at least one channel.

9. The system of claim 1 , wherein the filter is a polycarbonate filter.

10. The system of claim 1 , wherein the second chamber is connected to the outlet by a fluid connection channel.

11. The system of claim 10 , wherein no other channels connected to an additional outlet extend from the second chamber.

12. The system of claim 2 , wherein the outlet is a channel with an open end that provides access to the fluidic system, and wherein the filter is located between the upper component and the lower component.

13. The system of claim 1 , wherein the plurality of micropores are 5 um to 10 um in diameter.

14. The system of claim 1 , wherein the plurality of micropores are 8 um to 10 um in diameter.

15. The system of claim 1 , wherein the plurality of micropores are 5 um to 8 um in diameter.

16. A method for sorting sperm, comprising:
delivering a sample of sperm into an inlet of a fluidic system, wherein the fluidic system comprises a housing including a lower component and an upper component coupled together, and wherein the inlet extends through the upper component;
allowing at least some motile sperm in the sample of sperm, in a flow-free manner, to traverse a flow path in the fluidic system from the inlet through at least one channel to a first chamber and through a horizontally-arranged filter against gravity into a second chamber positioned above the first chamber, and then to traverse a path into an outlet, wherein the first chamber and the second chamber are separated by the filter, and wherein the filter has a plurality of micropores sized to permit a head of the sperm to pass therethrough; and
harvesting functional sperm suspended in a liquid from the fluidic system through the outlet, wherein the functional sperm are suitable for fertilization and are at least some of the motile sperm that have passed through the filter, wherein a percentage of motile sperm to non-motile sperm that are harvested at the outlet is greater than the percentage in the sample.

17. The method of claim 16 , wherein the filter is located between the upper component and the lower component.

18. The method of claim 17 , wherein a top portion of the second chamber comprises an opening and provides access to the fluidic system, in addition to the outlet.

19. The method of claim 18 , wherein sperm are located in the first chamber but not the second chamber.

20. The method of claim 17 , wherein the second chamber is connected to the outlet by a fluid connection channel, and wherein the plurality of micropores are 3 um to 10 um in diameter.

21. The method of claim 17 , wherein the outlet is a channel with an open end that provides access to the fluidic system, and wherein the plurality of micropores are 3 um to 10 um in diameter.

22. The method of claim 17 , wherein the outlet does not comprise a filter connected to another outlet when the at least some of the motile sperm are harvested.

23. The method of claim 16 , wherein the outlet is an open end of an additional collection chamber that is connected to the second chamber by a fluid connection channel, and wherein the plurality of micropores are 3 um to 10 um in diameter.

24. The method of claim 16 , wherein the sample of sperm is delivered into the first chamber, and wherein the outlet is a channel with an open end that provides access to the fluidic system.

25. The method of claim 16 , wherein the second chamber is connected to the outlet by a fluid connection channel, and wherein the plurality of micropores are 3 um to 10 um in diameter.

26. The method of claim 25 , wherein no other channels connected to an additional outlet extend from the second chamber.

27. The method of claim 16 , wherein the outlet does not comprise a filter connected to another outlet when the at least some of the motile sperm are harvested.

28. The method of claim 16 , wherein the plurality of micropores are at least 1 um and less than 10 um in diameter.

29. The method of claim 16 , wherein the first chamber extends through the lower component and the second chamber extends through the upper component.

30. The method of claim 16 , wherein the at least one channel is a straight channel that directly connects the inlet to the first chamber.

31. A method for sorting sperm, comprising:
delivering a sample of sperm into a fluidic system;
allowing at least some motile sperm in the sample of sperm, in a flow-free manner, to traverse a flow path in the fluidic system from a first chamber and through a filter against gravity into a second chamber positioned above the first chamber, and then to traverse a path into an outlet, wherein the first chamber and the second chamber are separated by the filter, and wherein the filter has a plurality of micropores sized to permit a head of the sperm to pass therethrough; and
harvesting functional sperm suspended in a liquid from the fluidic system through the outlet, wherein t

Assignees

• Brigham & Womens Hospital Inc

Inventors

• Demirci Utkan
• Asghar Waseem

Classifications

• G01N15/10 Primary

  Investigating individual particles · CPC title

• B01L3/502753

  characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation · CPC title

• C12N5/061

  Sperm cells, spermatogonia · CPC title

• C12N5/0612

  sorting of gametes, e.g. according to sex or motility · CPC title

• G01N1/4005

  by transferring a selected component through a membrane · CPC title