Recombinant vector comprising ERT2 fused to CAS9

Patent metadata

Field	Value
Publication number	US-11785922-B2
Application number	US-201816624575-A
Country	US
Kind code	B2
Filing date	May 3, 2018
Priority date	Jun 19, 2017
Publication date	Oct 17, 2023
Grant date	Oct 17, 2023

Abstract

Provided are a protein complex in which estrogen receptor 2 (-ERT2) is fused to CRISPR associated protein 9 (Cas9), and a recombinant vector carrying a gene coding the protein complex, wherein ERT2 is bonded to the N-terminus and C-terminus of nuclear localization sequence (NLS)-removed Cas9 and the complex has the advantage of translocating from the cytosol into the nucleus at a certain time point upon treatment with tamoxifen and modifying a specific DNA with the aid of guide RNA (gRNA), ultimately enabling a more elaborate DNA modification operation in a desired part at a desired time point.

First claim

The invention claimed is:

1. A recombinant vector comprising: (a) a U6 promoter and a guide RNA (gRNA), and (b) a cytomegalovirus (CMV) promoter and a gene encoding a protein complex, wherein the protein complex is encoded by a gene consisting of the nucleotide sequence of SEQ ID NO: 12, and wherein the U6 promoter, the gRNA, the CMV promoter, and the gene consisting of SEQ ID No: 12 are operatively linked within the recombinant vector.

Assignees

• Seoul Nat Univ Hospital

Inventors

• Lee Eun Ju
• Kim Hyo-Soo

Classifications

• A01K67/0275 Primary

  Genetically modified vertebrates, e.g. transgenic · CPC title

• C07K14/72

  for hormones {(for neuromediators C07K14/70571)} · CPC title

• C12N9/22 Primary

  Ribonucleases {[RNase]; Deoxyribonucleases [DNase]} · CPC title

• C12N15/113

  Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; {Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing (when used in plants C12N15/8218)} · CPC title

• C12N15/8509

  for producing genetically modified animals, e.g. transgenic · CPC title