Process of extracting oil from thin stillage

Patent metadata

Field	Value
Publication number	US-11781123-B2
Application number	US-201616062769-A
Country	US
Kind code	B2
Filing date	Dec 16, 2016
Priority date	Dec 22, 2015
Publication date	Oct 10, 2023
Grant date	Oct 10, 2023

Abstract

A process of recovering oil, comprising (a) converting a starch-containing material into dextrins with an alpha-amylase; (b) saccharifying the dextrins using a carbohydrate source generating enzyme to form a sugar; (c) fermenting the sugar in a fermentation medium into a fermentation product using a fermenting organism; (d) recovering the fermentation product to form a whole stillage; (e) separating the whole stillage into thin stillage and wet cake; (e′) optionally concentrating the thin stillage into syrup; (f) recovering oil from the thin stillage and/or optionally the syrup, wherein a phospholipase is present and/or added during steps (a) to (c). Use of phospholipase for increasing oil recovery yields from thin stillage and/or syrup in a fermentation product production process.

First claim

The invention claimed is:

1. A process of recovering oil, comprising:
(a) converting starch-containing material into dextrins with an alpha amylase, wherein the starch-containing material comprises corn;
(b) saccharifying the dextrins with a glucoamylase to form a sugar;
(c) fermenting the sugar in a fermentation medium with yeast into a fermentation product, wherein the fermentation product comprises ethanol;
(d) recovering the fermentation product to form a whole stillage;
(e) separating the whole stillage into thin stillage and wet cake;
(f) concentrating the thin stillage into syrup;
(g) recovering oil from the thin stillage and/or the syrup, wherein a phospholipase C is present and/or added during steps (a) to (c), and wherein oil yield is increased compared to a control process without phospholipase C.

2. The process of claim 1 , wherein the phospholipase C is present during steps (b) or (c).

3. The process of claim 1 , wherein the phospholipase C is present during steps (b) and (c).

4. The process of claim 1 , wherein the phospholipase C is added during steps (b) or (c).

5. The process of claim 1 , wherein the phospholipase C is added during steps (b) and (c).

6. The process of claim 1 , wherein one or more surfactants are present and/or added during steps (b) and/or (c).

7. The process of claim 6 , wherein the surfactants comprise non-ionic surfactants and are selected from the group consisting of: polyethylene glycol sorbitan monooleate, sorbitane monooleate, and a mixture thereof.

8. The process of claim 1 , comprising:
(a) converting the starch-containing material into dextrins with the alpha-amylase at a temperature above the initial gelatinization temperature;
(b) saccharifying the dextrins using the glucoamylase to form the sugar;
(c) fermenting the sugar in a fermentation medium into the fermentation product with the yeast;
(d) recovering the fermentation product to form the whole stillage;
(e) separating the whole stillage into thin stillage and wet cake;
(f) concentrating the thin stillage into syrup;
(g) recovering oil from the thin stillage and/or the syrup, wherein the phospholipase C is present and/or added during steps (b) and/or (c).

9. The process of claim 8 , wherein step (b) and step (c) are carried out sequentially.

10. The process of claim 8 , wherein step (b) and step (c) are carried out simultaneously.

11. The process of claim 10 , wherein the phospholipase C is present during steps (b) and (c).

12. The process of claim 10 , wherein the phospholipase C is added during steps (b) and (c).

13. The process of claim 1 , comprising:
(a) converting the starch-containing material into dextrins with the alpha-amylase at a temperature below the initial gelatinization temperature;
(b) saccharifying the dextrins with the glucoamylase to form the sugar;
(c) fermenting the sugar in the fermentation medium into the fermentation product with the yeast;
(d) recovering the fermentation product to form the whole stillage;
(e) separating the whole stillage into thin stillage and wet cake;
(f) concentrating the thin stillage into syrup;
(g) recovering oil from the thin stillage and/or the syrup, wherein the phospholipase C is present and/or added during steps (a) to (c).

14. The process of claim 13 , wherein step (b) and step (c) are carried out sequentially.

15. The process of claim 13 , wherein step (b) and step (c) are carried out simultaneously.

16. The process of claim 13 , wherein steps (a), (b) and (c) are carried out sequentially at the temperature below the initial gelatinization temperature.

17. The process of claim 13 , wherein steps (a), (b) and (c) are carried out simultaneously at the temperature below the initial gelatinization temperature.

18. The process of claim 17 , wherein the phospholipase C is present during steps (a), (b) and (c).

19. The process of claim 17 , wherein the phospholipase C is added during steps (a), (b) and (c).

Assignees

• Novozymes As

Inventors

• Akerman Michael John
• Kreel Nathaniel Edward
• Hooss Melissa Carrie
• Shen Xinyu

Classifications

• C12N9/14 Primary

  Hydrolases (3) · CPC title

• C12N9/16 Primary

  acting on ester bonds (3.1) · CPC title

• C12N9/20

  Triglyceride splitting, e.g. by means of lipase · CPC title

• C12N9/2414

  Alpha-amylase (3.2.1.1.) · CPC title

• C12N9/2417

  from microbiological source · CPC title