System, method, and device for forming an array of emulsions

Patent metadata

Field	Value
Publication number	US-11779925-B2
Application number	US-202117549163-A
Country	US
Kind code	B2
Filing date	Dec 13, 2021
Priority date	Feb 13, 2017
Publication date	Oct 10, 2023
Grant date	Oct 10, 2023

Abstract

Systems, methods, and devices for forming an array of emulsions. An exemplary device comprises a frame and at least one or a plurality of separate microfluidic modules mounted to the frame and each configured to form an array of emulsions. In some embodiments, each module may be mounted by snap-fit attachment. The device also may include the same sealing member bonded to a top side of each module and hermetically sealing each of the modules. Another exemplary microfluidic device for forming an array of emulsions comprises a stack of layers bonded together. The stack may comprise a port layer forming a plurality of ports. Each port may have a top rim formed by a protrusion that encircles the central axis of the port. The rims may be coplanar with one another to facilitate bonding of a sealing member to each rim.

First claim

We claim:

1. A method of forming emulsions, the method comprising:
selecting a device including a frame and a plurality of discrete microfluidic modules, each microfluidic module being mounted to the frame and including an array of emulsion formation units;
loading emulsion formation units of each array with sample-containing fluid;
selecting a sealing member;
bonding the sealing member to each microfluidic module of the plurality of microfluidic modules; and
applying vacuum and/or pressure through the sealing member to the array of emulsion formation units of each microfluidic module, to generate droplets of sample-containing fluid encapsulated by carrier fluid, using emulsion formation units of the microfluidic module.

2. The method of claim 1 , wherein the device selected has each microfluidic module attached to the frame via complementary mating features of the microfluidic module and the frame.

3. The method of claim 2 , further comprising mounting each microfluidic module to the frame via snap-fit attachment before selecting.

4. The method of claim 1 , wherein each emulsion formation unit includes a sample reservoir and a sample-loading port overlying the sample reservoir, wherein loading includes loading sample-containing fluid into the sample reservoir via the sample-loading port, and sealing includes sealing an opening of the sample-loading port to be fluid-tight using the sealing member, such that fluid cannot enter or exit the sample-loading port via the opening.

5. The method of claim 1 , wherein bonding includes bonding the sealing member directly to one or more protrusions formed on a top side of each microfluidic module, and wherein the one or more protrusions form a top rim of a plurality of ports of the microfluidic module.

6. The method of claim 1 , wherein each emulsion formation unit has a plurality of ports each defining an opening, and wherein bonding includes sealing the opening of each port of the plurality of ports to be fluid-tight using the sealing member, such that fluid cannot enter or exit the port via the opening.

7. The method of claim 6 , wherein the plurality of ports includes a plurality of sample-loading ports, at least one carrier fluid port, and at least one vacuum/pressure port.

8. The method of claim 7 , wherein the plurality of ports also includes a respective vent port for each emulsion formation unit.

9. The method of claim 1 , further comprising forming openings in the sealing member after bonding, to access, through the sealing member, at least one carrier fluid port of each microfluidic module, at least one vacuum/pressure port of the microfluidic module, and a respective vent port for each emulsion formation unit of the microfluidic module.

10. The method of claim 9 , wherein forming openings includes piercing the sealing member.

11. The method of claim 1 , wherein applying vacuum and/or pressure includes applying vacuum to only a single line of vacuum ports defined collectively by the plurality of microfluidic modules.

12. The method of claim 1 , wherein each microfluidic module has a plurality of sample reservoirs to receive sample-containing fluid and forms a channel network in which droplets of sample-containing fluid are formed, and wherein the channel network is located above a lower portion of the sample reservoir of the microfluidic module.

13. The method of claim 1 , wherein each microfluidic module includes a plurality of layers nonremovably bonded to one another and forming each emulsion formation unit of the microfluidic module, and wherein applying pressure/vacuum includes applying pressure or vacuum to each emulsion formation unit of the microfluidic module via a manifold created by one or more layers of the plurality of layers.

14. The method of claim 13 , wherein each microfluidic module includes a plurality of sample reservoirs to receive sample-containing fluid, wherein the plurality of layers includes a well layer forming a lower portion of each sample reservoir of the plurality of sample reservoirs, a channel layer located over the well layer and forming a droplet generation site of each emulsion formation unit of the microfluidic module, and a port layer located over the channel layer and forming a plurality of ports arranged in fluid communication with the emulsion formation units of the microfluidic module, and wherein applying pressure/vacuum includes applying pressure or vacuum to each emulsion formation unit of the microfluidic module via a vacuum/pressure port formed by the port layer.

15. The method of claim 14 , wherein the port layer defines a respective sample-loading port over the sample reservoir of each emulsion formation unit of the microfluidic module, a vent port for the sample reservoir, and a channel that fluidically connects the vent port to the sample-loading port, wherein the port layer includes a body having a top surface and also includes at least one protrusion projecting upwardly from the top surface and formed integrally with the body, wherein the at least one protrusion forms an encircling rim of each sample-loading port and each vent port, and wherein sealing includes bonding the sealing member directly to the encircling rim of each sample-loading port and each vent port.

Assignees

• Bio Rad Laboratories Inc

Inventors

• Cauley Iii Thomas Henry
• Dzenitis John
• Hobbs Steve
• Schiaffino Stefano

Classifications

• B01L3/502784 Primary

  specially adapted for droplet or plug flow, e.g. digital microfluidics · CPC title

• B01F23/4105

  Methods of emulsifying · CPC title

• B01F33/85

  Mixing plants with mixing receptacles or mixing tools that can be indexed into different working positions · CPC title

• B01L3/502715

  characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces · CPC title

• G01N1/38

  Diluting, dispersing or mixing samples · CPC title