Inductively coupled plasma based atomic analysis systems and methods

Patent metadata

Field	Value
Publication number	US-11776801-B2
Application number	US-202117470769-A
Country	US
Kind code	B2
Filing date	Sep 9, 2021
Priority date	Sep 18, 2020
Publication date	Oct 3, 2023
Grant date	Oct 3, 2023

Abstract

Inductively coupled plasma (ICP) analyzers use an ICP torch to generate a plasma in which a sample is atomized an ionized. Analysis of the atomic ions can be performed by atomic analysis, such as mass spectrometry (MS) or atomic emission spectrometry (AES). Particle based ICP analysis includes analysis of particles such as cells, beads, or laser ablation plumes, by atomizing and ionizing particles in an ICP torch followed by atomic analysis. In mass cytometry, mass tags of particles are analyzed by mass spectrometry, such as by ICP-MS. Systems and methods of the subject application include one or more of: a demountable ICP torch holder assembly, an external ignition device; an ICP load coil comprising an annular fin, particle suspension sample introduction fluidics, and ICP analyzers thereof.

First claim

What is claimed is:

1. An inductively coupled plasma (ICP) torch box comprising:
a demountable ICP torch holder assembly;
an ICP load coil comprising an annular fin, wherein the load coil is a cylindrical coil, and the annular fin is contiguous for at least an eighth of a turn of the cylindrical coil; and
an external ignition device that ignites a plasma by dielectric barrier discharge.

2. An inductively coupled plasma (ICP) torch box comprising:
(a) a demountable inductively coupled plasma (ICP) torch holder assembly, comprising:
an inner torch body comprising an inner tube coupled to an inner tube base;
an outer torch body comprising an outer tube coupled to an outer tube base;
a torch holder retaining a demountable inner torch base and a demountable outer torch base;
(b) an ICP load coil positioned around the outer tube, comprising:
a cylindrical coil;
wherein the cylindrical coil comprises an annular fin;
wherein the annular fin is contiguous for at least an eighth of a turn of the cylindrical coil;
wherein the ICP load coil predominantly comprises a metal or alloy that has a lower rate of oxidation during operation than copper;
(c) an external ignition device for igniting a plasma in an inductively coupled plasma (ICP) torch, the ignition device comprising:
a circuit comprising:
an oscillator;
a first high voltage transformer coupled to a first electrode;
a second high voltage transformer coupled to a second electrode;
wherein the ignition device is configured to ignite the plasma by dielectric barrier discharge; and
wherein the first and second electrodes are positioned on opposite sides of the outer tube and within 5 millimeters of the outer tube.

3. A demountable inductively coupled plasma (ICP) torch holder assembly, comprising:
an inner torch body comprising an inner tube coupled to an inner tube base;
an outer torch body comprising an outer tube coupled to an outer tube base; and
a torch holder configured to receive the inner tube base and the outer tube base;
wherein the inner tube and the outer tube define an annular region when the torch holder retains the inner tube base and the outer tube base, and
wherein:
(a) the outer tube detaches from the outer tube base,
(b) the inner tube detaches from the inner tube base,
(c) the torch holder defines an outer gas inlet, the outer gas inlet is in fluid communication with the annular region, the outer tube base defines three or more holes positioned to provide fluid communication between the outer gas inlet and the annular region, and the three or more holes are oriented to create a vortex flow,
(d) the inner tube base and the outer tube base are permanently joined to each other,
(e) the torch holder comprises a thermally conductive element positioned to heat an injector,
(f) a metal is the predominant material of the torch holder,
(g) an aluminum alloy is the predominant material of the torch holder, or
(h) the torch holder assembly further comprises a gas supply manifold configured to receive the torch holder, the torch holder comprises a pin to align its position in the gas supply manifold, and twisting the torch holder allows removal of the torch holder from the gas supply manifold.

4. The torch holder assembly of claim 3 , wherein the outer tube detaches from the outer tube base.

5. The torch holder assembly of claim 3 , wherein the inner tube detaches from the inner tube base.

6. The torch holder assembly of claim 3 , wherein the torch holder defines the outer gas inlet, the outer gas inlet is in fluid communication with the annular region, the outer tube base defines three or more holes positioned to provide fluid communication between the outer gas inlet and the annular region, and the three or more holes are oriented to create a vortex flow.

7. The torch holder assembly of claim 6 , wherein the outer tube base defines six holes.

8. The torch holder assembly of claim 6 , wherein the holes are within 2.5 cm of an outlet of the inner tube.

9. The torch holder assembly of claim 3 , wherein the outer tube does not comprise a taper.

10. The torch holder assembly of claim 3 , wherein the inner tube base and the outer tube base are each separately demountable from the torch holder.

11. The torch holder assembly of claim 3 , wherein the inner tube base and the outer tube base are permanently joined to each other.

12. The torch holder assembly of claim 3 , wherein the inner tube base and the outer tube base are configured to be connected by a fastener.

13. The torch holder assembly of claim 3 , wherein the torch holder comprises the thermally conductive element positioned to heat the injector.

14. The torch holder assembly of claim 3 , wherein the metal is the predominant material of the torch holder.

15. The torch holder assembly of claim 3 , wherein the aluminum alloy is the predominant material of the torch holder.

16. The torch holder assembly of claim 3 , wherein the outer tube does not comprise a hole for extending an electrode into the outer tube.

17. The torch holder assembly of claim 3 , wherein the torch holder assembly further comprises the gas supply manifold configured to receive the torch holder, the torch holder comprises the pin to align its position in the gas supply manifold, and twisting the torch holder allows removal of the torch holder from the gas supply manifold.

18. The torch holder assembly of claim 17 , wherein the torch holder defines the outer gas inlet, wherein the outer gas inlet is in fluid communication with the annular region and with an outer gas flow region of the gas supply manifold.

19. The torch box of claim 1 , wherein the demountable ICP torch holder assembly comprises:
an inner torch body comprising an inner tube coupled to an inner tube base,
an outer torch body comprising an outer tube coupled to an outer tube base, and
a torch holder retaining a demountable inner torch base and a demountable outer torch base.

20. The torch box of claim 2 , wherein the outer tube is reversibly detachable from the outer tube base.

Assignees

• Fluidigm Canada Inc
• Standard Biotools Canada Inc

Inventors

• Loboda Alexander
• Jong Raymond
• Sullivan Michael
• Vorobiev Serguei
• Rotenberg Robert
• Stratulativ Emil D
• Voronov Maxim
• Armstrong Mark

Classifications

• G01N2570/00

  Omics, e.g. proteomics, glycomics or lipidomics; Methods of analysis focusing on the entire complement of classes of biological molecules or subsets thereof, i.e. focusing on proteomes, glycomes or lipidomes · CPC title

• G01N33/58

  involving labelled substances (G01N33/53 takes precedence) · CPC title

• C12M23/16

  Microfluidic devices; Capillary tubes (integrated microfluidic structures B01L3/5027; microreactors B01J19/0093) · CPC title

• H05H2242/24

  Radiofrequency or microwave generators · CPC title

• H01J49/105 Primary

  using high-frequency excitation, e.g. microwave excitation, Inductively Coupled Plasma [ICP] · CPC title