Integrated biochemical sensor and method of manufacturing the same

Patent metadata

Field	Value
Publication number	US-11774439-B2
Application number	US-202117212248-A
Country	US
Kind code	B2
Filing date	Mar 25, 2021
Priority date	Dec 9, 2020
Publication date	Oct 3, 2023
Grant date	Oct 3, 2023

Abstract

Disclosed is an integrated biochemical sensor including a reference electrode, a plurality of working electrodes each having different artificial lipid membranes, and partition layers for electrically insulating the reference electrode and each of the working electrodes.

First claim

What is claimed is:

1. An integrated biochemical sensor, comprising:
a plurality of working electrode layers;
an insulating encapsulation layer including a central portion and a plurality of first openings on the working electrode layers, the first openings being along a periphery of the central portion and corresponding to the working electrode layers, respectively, the insulating encapsulation layer configured to electrically insulate each of the working electrode layers and a reference electrode layer;
the reference electrode layer on the central portion of the insulating encapsulation layer;
a partition structure including a plurality of second openings on the insulating encapsulation layer and electrically insulating the reference electrode layer and the working electrode layers from each other, the partition structure exposing the reference electrode layer and the working electrode layers through the second openings;
a plurality of working electrode structures each having different artificial lipid membranes and being on the working electrode layers, respectively; and
a reference electrode structure on the reference electrode layer,
wherein a reference electrode comprising the reference electrode layer and the reference electrode structure is in a central region, and
wherein working electrodes comprising respective pairs of the working electrode layers and the plurality of working electrode structures are in a plurality of regions around an outer circumferential surface of the reference electrode, respectively.

2. The integrated biochemical sensor according to claim 1 , wherein membrane potentials of the artificial lipid membranes change due to chemical reaction with biochemical ions corresponding to the artificial lipid membranes.

3. The integrated biochemical sensor according to claim 2 , wherein the biochemical ions are detected based on a potential difference between the reference electrode and respective one of the working electrodes.

4. The integrated biochemical sensor according to claim 1 , wherein each of the working electrodes further comprises a corresponding one of the working electrode layer, a stabilizing layer, and an electrolyte layer for improving electrical connectivity between the corresponding one of the working electrode layers and a corresponding one of the artificial lipid membranes.

5. The integrated biochemical sensor according to claim 4 , wherein the stabilizing layer comprises at least one of silver (Ag), silver chloride (AgCl), and polyvinyl butyral (PVB).

6. The integrated biochemical sensor according to claim 4 , wherein the electrolyte layer comprises at least one of poly(2-hydroxyethyl methacrylate) (pHEMA) and Poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) PEDOT:PSS).

7. The integrated biochemical sensor according to claim 1 , further comprising:
a lower encapsulation layer for protecting the working electrodes from external impact under the working electrode layers,
the insulating encapsulation layer for electrically insulating each of the working electrodes and the reference electrode on the working electrode layers, and
an upper encapsulation layer for protecting the reference electrode from external impact on the insulating encapsulation layer and under the partition structure.

8. The integrated biochemical sensor according to claim 1 , wherein
the artificial lipid membranes include a first artificial lipid membrane, a second artificial lipid membrane, a third artificial lipid membrane, and a fourth artificial lipid membrane, and
the working electrodes comprise a first working electrode for detecting biochemical ions corresponding to saltiness through the first artificial lipid membrane, a second working electrode for detecting biochemical ions corresponding to bitterness through the second artificial lipid membrane, a third working electrode for detecting biochemical ions corresponding to sweetness through the third artificial lipid membrane, and a fourth working electrode for detecting biochemical ions corresponding to sourness through the fourth artificial lipid membrane.

9. The integrated biochemical sensor according to claim 8 , wherein the first artificial lipid membrane comprises at least one of tetradodecylammonium, bromide 1-hexadecanol, and di-n-octyl phenylphosphonate.

10. The integrated biochemical sensor according to claim 8 , wherein the second artificial lipid membrane comprises at least one of methyl trioctyl ammonium chloride and di-n-octyl phenylphosphonate.

11. The integrated biochemical sensor according to claim 8 , wherein the third artificial lipid membrane comprises at least one of tetradodecylammonium bromide and di-n-octyl phenylphosphonate.

12. The integrated biochemical sensor according to claim 8 , wherein the fourth artificial lipid membrane comprises at least one of methyl trioctyl ammonium chloride, oleic acid, bis(2-ethylhexyl) phosphate, and dioctyl phenyl phosphonate.

13. A method of manufacturing an integrated biochemical sensor, comprising:
forming a plurality of working electrode layers;
forming an insulating encapsulation layer including a central portion and a plurality of first openings on the working electrode layers, the first openings being along a periphery of the central portion and corresponding to the working electrode layers, respectively, such that the insulating encapsulation layer electrically insulates each of the working electrode layers and a reference electrode layer;
forming the reference electrode layer on the central portion of the insulating encapsulation layer;
forming an upper encapsulation layer on the insulating encapsulation layer for protecting the reference electrode layer from external impact on the insulating encapsulation layer;
forming a partition structure including a plurality of second openings on the insulating encapsulation layer on the upper encapsulation layer such that the partition structure electrically insulates the reference electrode layer and the working electrode layers from each other, and exposes the reference electrode layer and the working electrode layers through the second openings; and
forming artificial lipid membranes on the working electrode layers,
wherein the reference electrode layer is in a central region, and
wherein the working electrode layers are in a plurality of regions around an outer circumferential surface of the reference electrode layer, respectively.

14. The method according to claim 13 , wherein the forming of the artificial lipid membranes comprises:
forming a stabilizing layer on the working electrode layers and the reference electrode layer;
forming an electrolyte layer on the working electrode layers on which the stabilizing layer has been formed and the reference electrode layer on which the stabilizing layer has been formed; and
forming the artificial lipid membranes on the working electrode layers on which the electrolyte layer has been formed.

Assignees

• Daegu Gyeongbuk Inst Science & Tech

Inventors

• Jang Kyung In
• Yea Jun Woo
• Jung Han Hee

Classifications

• G01N33/48728 Primary

  Investigating individual cells, e.g. by patch clamp, voltage clamp (investigating individual particles in general G01N15/10) · CPC title

• G01N27/327 Primary

  Biochemical electrodes {, e.g. electrical or mechanical details for in vitro measurements} · CPC title

• G01N27/403

  Cells and electrode assemblies · CPC title

• G01N27/4163

  checking the operation of, or calibrating, the measuring apparatus (G01N27/3274, G01N27/4175 and G01N33/0006 take precedence) · CPC title

• G01N27/27

  Association of two or more measuring systems or cells, each measuring a different parameter, where the measurement results may be either used independently, the systems or cells being physically associated, or combined to produce a value for a further parameter · CPC title