Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes
US-2016057967-A1 · Mar 3, 2016 · US
bZIP transcription factors regulate conversion of nicotine to nornicotine
US11773401B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11773401-B2 |
| Application number | US-201816770906-A |
| Country | US |
| Kind code | B2 |
| Filing date | Dec 6, 2018 |
| Priority date | Dec 7, 2017 |
| Publication date | Oct 3, 2023 |
| Grant date | Oct 3, 2023 |
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- Title
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Abstract
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A method of decreasing conversion of nicotine to nornicotine is provided herein. The methods includes administering at least one basic region/leucine zipper (bZIP) type transcription factor inhibitor to an organism in need thereof. Also provided herein is a method of decreasing conversion of nicotine to nornicotine including mutating a bZIP type transcription factor binding site on a promoter of a nicotine N-demethylase (NND). Further provided herein is a method of decreasing conversion of nicotine to nornicotine including mutating a plant genome to knockout at least one bZIP type transcription factor.
First claim
Opening claim text (preview).
What is claimed is: 1. A method of decreasing conversion of nicotine to nornicotine in a Nicotiana plant, the method comprising mutating a plant genome to knockout a Nicotiana basic region/leucine zipper (bZIP) transcription factor comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 2, 4, 5, and 6; and measuring expression of genes encoding nicotine demethylases, measuring nicotine levels, measuring nornicotine levels, and/or measuring N-nitrosonornicotine levels in the Nicotiana plant. 2. The method of claim 1 , wherein the Nicotiana bZIP transcription factor is a group C bZIP transcription factor. 3. The method of claim 1 , wherein the Nicotiana bZIP transcription factor is NtbZIP1a (SEQ ID NO: 2). 4. The method of claim 1 , comprising mutating the plant genome to knockout at least two of the Nicotiana bZIP transcription. 5. The method of claim 1 , wherein the Nicotiana bZIP transcription factor is a group S bZIP transcription factor. 6. The method of claim 1 , wherein the Nicotiana bZIP transcription factor is NtbZIP1b (SEQ ID NO: 4). 7. The method of claim 1 , wherein the Nicotiana bZIP transcription factor is NtbZIP2a (SEQ ID NO: 5). 8. The method of claim 1 , wherein the Nicotiana bZIP transcription factor is NtbZIP2b (SEQ ID NO: 6).
Assignees
Inventors
Classifications
- C12N15/8218Primary
Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS] · CPC title
Leaves · CPC title
Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; {Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing (when used in plants C12N15/8218)} · CPC title
Antisense · CPC title
MicroRNAs, miRNAs · CPC title
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- What does patent US11773401B2 cover?
- A method of decreasing conversion of nicotine to nornicotine is provided herein. The methods includes administering at least one basic region/leucine zipper (bZIP) type transcription factor inhibitor to an organism in need thereof. Also provided herein is a method of decreasing conversion of nicotine to nornicotine including mutating a bZIP type transcription factor binding site on a promoter o…
- Who is the assignee on this patent?
- Univ Kentucky Res Found, Reynolds Tobacco Co R
- What technology area does this patent fall under?
- Primary CPC classification C12N15/8218. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Oct 03 2023 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 1 related publication on this page (citations in our corpus or others sharing the same primary CPC).