Crosslinking agent, preparation process and uses thereof, hydrogel and biodegradable cryogel comprising crosslinking agent

Patent metadata

Field	Value
Publication number	US-11773205-B2
Application number	US-202016998652-A
Country	US
Kind code	B2
Filing date	Aug 20, 2020
Priority date	Aug 20, 2020
Publication date	Oct 3, 2023
Grant date	Oct 3, 2023

Abstract

The present disclosure provides a crosslinking agent, the preparation process and uses thereof, a hydrogel and a biodegradable cryogel including the crosslinking agent.

First claim

What is claimed is:

1. A biodegradable cryogel obtained by reacting a crosslinking agent comprising a plurality of difunctional polyurethane nanoparticles wherein each of the difunctional polyurethane nanoparticles has a plurality of aldehyde groups with a polymer having amine groups and being placed at a temperature of −17° C. to −25° C.; wherein the polymer having amine groups is chitosan or its derivatives; wherein the biodegradable cryogel upon subcutaneous injection, becomes covered with immune cells on and around it, and wherein said immune cells comprise macrophages, wherein the ratio of M2 macrophages to M1 macrophages is more than three to about 3.5.

2. The biocompatible cryogel according to claim 1 , which is prepared by a 3D printing method.

3. The biocompatible cryogel according to claim 1 , wherein the biocompatible cryogel has a shape memory property.

4. The biocompatible cryogel according to claim 1 , wherein the cryogel is used to culture cells.

5. The biodegradable cryogel according to claim 1 , wherein the biocompatible cryogel has a self-healing property.

6. A hydrogel obtained by reacting by reacting a crosslinking agent comprising a plurality of difunctional polyurethane nanoparticles wherein each of the difunctional polyurethane nanoparticles has a plurality of aldehyde groups with a polymer having amine groups; wherein the polymer having amine groups is chitosan or its derivatives; wherein the hydrogel has a storage modulus and a loss modulus, and the storage modulus and the loss modulus are measured by damage-healing cycles at strain of 500% for damage and at strain of 1% for healing; upon subcutaneous injection, becomes covered with immune cells on and around it, and wherein said immune cells comprise macrophages, wherein the ratio of M2 macrophages to M1 macrophages was more than three to about 3.5.

7. The hydrogel according to claim 6 , which has a self-healing property.

8. The hydrogel according to claim 6 , which is used to culture cells, and is biocompatible.

Assignees

• Univ Nat Taiwan

Inventors

• Hsu Shan-Hui
• Lin Tzu-Wei

Classifications

• C12N5/0645

  Macrophages, e.g. Kuepfer cells in the liver; Monocytes · CPC title

• C08G18/4286 Primary

  prepared from a combination of hydroxycarboxylic acids and/or lactones with polycarboxylic acids or ester forming derivatives thereof and polyhydroxy compounds · CPC title

• A61K9/0019

  Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner (non-active ingredients are additionally classified in A61K47/00) · CPC title

• A61K9/06

  Ointments; Bases therefor; {Other semi-solid forms, e.g. creams, sticks, gels (composition of ointments, creams or gels A61K47/00)} · CPC title

• A61K31/785 Primary

  Polymers containing nitrogen · CPC title