Methods of mass spectrometry quantitation using cleavable isobaric tags and neutral loss fragmentation

Patent metadata

Field	Value
Publication number	US-11764043-B2
Application number	US-201816028932-A
Country	US
Kind code	B2
Filing date	Jul 6, 2018
Priority date	Jul 6, 2017
Publication date	Sep 19, 2023
Grant date	Sep 19, 2023

Abstract

Isobaric mass spectrometry tags (e.g., TMT) are susceptible to ratio compression, which arises from the co-isolation and co-fragmentation of interfering species that also contribute to the final reporter ion ratios. Additional stages of ion activation/transformation (e.g., MSn and PTR) have been shown to decrease ratio compression. Embodiments of the present invention include a mass spectrometry cleavable moiety on the isobaric mass tags. The cleavable moiety allows for a predictable mass loss, and results in an improved tag reporter ion purity.

First claim

What is claimed is:

1. A method of mass spectrometry, comprising:
(a) labeling a peptide with an isobaric tag comprising a tag reporter moiety, a neutral loss moiety and a mass balance region to form a tagged peptide;
(b) ionizing the tagged peptide to form a precursor ion;
(c) selecting the precursor ion for fragmentation;
(d) in a first fragmentation, fragmenting the precursor ion to form a first generation of fragment ions and a neutral loss fragment, wherein the neutral loss fragment is, or is at least a part of, the neutral loss moiety, and in a first mass analysis, mass analyzing the first generation of ions and the neutral loss fragment;
(e) selecting at least one of the first generation of fragment ions for fragmentation; and
(f) in a second fragmentation, fragmenting the at least one first generation of fragment ions to form a second generation of fragment ions, wherein the second generation of fragment ions includes a tag reporter ion, and in a second mass analysis, mass analyzing the tag reporter ion, wherein the first mass analysis and the second mass analysis are the only mass analyses.

2. The method of claim 1 , wherein the peptide is labeled with a plurality of isobaric tags.

3. The method of claim 1 , wherein a plurality of peptides are labeled with a series of isobaric tags, wherein the plurality of peptides may be from the same source or from different sources and where the plurality of peptides have the same amino acid sequence.

4. The method of claim 1 , wherein ionizing the tagged peptide is performed using ESI, APCI, MALDI or APPI.

5. The method of claim 1 , wherein the peptide is sequenced concurrently or after the tag reporters are mass analyzed.

6. The method of claim 1 , wherein fragmenting the precursor ion to form a first generation of fragment ions is performed using UVPD.

7. The method of claim 1 , wherein fragmenting the precursor ion to form a first generation of fragment ions is performed using HCD, CID, ETD, NETD or ECD.

8. The method of claim 1 , wherein fragmenting the precursor ion to form a first generation of fragment ions is performed using IRMPD.

9. The method of claim 1 , wherein fragmenting at least one of the first generation of fragment ions to form a second generation of fragment ions is performed using PQD.

10. The method of claim 1 , wherein fragmenting the first generation of fragment ions to form a second generation of fragment ions is performed using HCD, IRMPD or CID.

11. The method of claim 1 , wherein fragmenting the first generation of fragment ions to form a second generation of fragment ions is performed using ETD, NETD or ECD.

12. The method of claim 1 , wherein fragmenting the first generation of fragment ions to form a second generation of fragment ions is performed using HCD or IRMPD.

13. The method of claim 1 , wherein the first and/or second generations of fragment ions are mass analyzed in an orbitrap mass analyzer.

14. The method of claim 1 , wherein the first and second generations of fragment ions are mass analyzed in a quadrupole, ion trap, FT-ICR or time of flight mass analyzer.

15. The method of claim 1 , wherein the neutral loss fragment is attached to a mass balance region of the isobaric tag.

16. The method of claim 1 , wherein the neutral loss fragment is attached to the tag reporter moiety of the isobaric tag.

17. The method of claim 2 , wherein the peptide labeled with multiple tags is fragmented using UVPD with resonant excitation whereby all precursor ions undergo only a single neutral loss.

18. A method of mass spectrometry, comprising:
(a) labeling a peptide with an isobaric tag comprising a tag reporter moiety, a neutral loss moiety and a mass balance region to form a tagged peptide;
(b) ionizing the tagged peptide to form a precursor ion;
(c) selecting the precursor ion for fragmentation;
(d) fragmenting the precursor ion to form a first generation of fragment ions and a neutral loss fragment, wherein the neutral loss fragment is, or is at least a part of, the neutral loss moiety;
(e) selecting at least one of the first generation of fragment ions for fragmentation;
(f) fragmenting the at least one first generation of fragment ions to form a second generation of fragment ions, wherein the second generation of fragment ions includes a tag reporter ion; and
(g) mass analyzing the tag reporter ion, wherein the isobaric tag is,

19. A method of mass spectrometry, comprising:
(a) labeling an analyte with an isobaric tag comprising a tag reporter moiety, a neutral loss moiety and a mass balance region to form a tagged analyte;
(b) ionizing the tagged analyte to form a precursor ion;
(c) selecting the precursor ion for fragmentation;
(d) in a first fragmentation, fragmenting the precursor ion to form a first generation of fragment ions and a neutral loss fragment, wherein the neutral loss fragment is or is at least a part of the neutral loss moiety, and in a first mass analysis, mass analyzing the first generation of ions and the neutral loss fragment;
(e) selecting at least one of the first generation of fragment ions for fragmentation; and
(f) in a second fragmentation, fragmenting the at least one first generation of fragment ions to form a second generation of fragment ions, wherein the second generation of fragment ions includes a tag reporter ion, and in a second mass analysis, mass analyzing the tag reporter ion,
wherein the first mass analysis and the second mass analysis are the only mass analysis.

20. The method of claim 19 wherein the analyte is a protein, peptoid, glycan, lipid, fatty acid, small molecule, polymer or nucleic acid.

Assignees

• Thermo Finnigan Llc

Inventors

• Senko Michael W
• Mcalister Graeme
• Etienne Christopher L

Classifications

• G01N33/6848 Primary

  Methods of protein analysis involving mass spectrometry · CPC title

• H01J49/0031 Primary

  Step by step routines describing the use of the apparatus (H01J49/0081 takes precedence) · CPC title

• G01N2458/15

  Non-radioactive isotope labels, e.g. for detection by mass spectrometry · CPC title