Methods for identifying and/or selecting wheat plants having a mutation in an endogenous Ms1 gene

Patent metadata

Field	Value
Publication number	US-11761016-B2
Application number	US-201916697681-A
Country	US
Kind code	B2
Filing date	Nov 27, 2019
Priority date	Sep 26, 2014
Publication date	Sep 19, 2023
Grant date	Sep 19, 2023

Abstract

Compositions and methods are capable of modulating male fertility in a plant. Compositions comprise polynucleotides and polypeptides, and fragments and variants thereof, which modulate male fertility. Expression cassettes comprise a male-fertility polynucleotide, or fragment or variant thereof, operably linked to a promoter, wherein expression of the polynucleotide modulates the male fertility of a plant. Regulatory sequences drive expression in a male-tissue-preferred manner and may be targets to downregulate an operably-linked gene. Mutations that induce nuclear recessive male sterility in subsequent selfing and crossing of wheat lines containing the mutations may be tracked. Male-sterile plants may be maintained by crossing with a maintainer plant. Hybrids can be produced using the male-sterile plants.

First claim

That which is claimed:

1. A method for identifying and/or selecting in at least one wheat plant that is homozygous or heterozygous for a mutation in an endogenous Ms1 gene, wherein said mutation induces nuclear recessive male sterility, said method comprising:
a. detecting at least one marker allele that is linked to and associated with a mutation in the endogenous Ms1 gene, wherein said marker allele linked to and associated with a mutation in the endogenous Ms1 gene is an allele at marker ET0487 as set forth in SEQ ID NO:24, ET0488 as set forth in SEQ ID NO:25, ET0489 as set forth in SEQ ID NO:26, ET0490 as set forth in SEQ ID NO:27, ET0491 as set forth in SEQ ID NO:28, ET0495 as set forth in SEQ ID NO:29, or 007-0033.1 as set forth in SEQ ID NO: 32; and
b. selecting the at least one wheat plant having the at least one marker allele.

2. The method of claim 1 , wherein said endogenous Ms1 gene encodes the polypeptide of SEQ ID NO:5.

Assignees

• Pioneer Hi Bred Int

Inventors

• Albertsen Marc C
• Baumann Ute
• Cigan Andrew Mark
• Singh Manjit
• Tucker Elise
• Whitford Ryan

Classifications

• A01H6/4678

  Triticum sp. [wheat] · CPC title

• A01H5/10

  Seeds · CPC title

• A01H1/045

  using molecular markers · CPC title

• A01H1/023

  Male sterility · CPC title

• C12N15/8289 Primary

  Male sterility · CPC title