Dendritic macroporous hydrogels prepared by crystal templating

Patent metadata

Field	Value
Publication number	US-11760858-B2
Application number	US-202117231653-A
Country	US
Kind code	B2
Filing date	Apr 15, 2021
Priority date	Feb 26, 2008
Publication date	Sep 19, 2023
Grant date	Sep 19, 2023

Abstract

The present invention includes a hydrogel and a method of making a porous hydrogel by preparing an aqueous mixture of an uncrosslinked polymer and a crystallizable molecule; casting the mixture into a vessel; allowing the cast mixture to dry to form an amorphous hydrogel film; seeding the cast mixture with a seed crystal of the crystallizable molecule; growing the crystallizable molecule into a crystal structure within the uncrosslinked polymer; crosslinking the polymer around the crystal structure under conditions in which the crystal structure within the crosslinked polymer is maintained; and dissolving the crystals within the crosslinked polymer to form the porous hydrogel.

First claim

What is claimed is:

1. A composition comprising:
a hydrogel composition;
wherein the hydrogel composition includes: (a) uncrosslinked hyaluronic acid; (b) uncrosslinked alginate located around the uncrosslinked hyaluronic acid; and (c) a cell adhesive protein;
wherein the uncrosslinked alginate is configured to be crosslinked around the uncrosslinked hyaluronic acid with an ion;
wherein the uncrosslinked hyaluronic acid is not configured to be crosslinked with the ion.

2. The composition of claim 1 , wherein the ion is calcium.

3. The composition of claim 2 , wherein the uncrosslinked alginate includes sodium alginate.

4. The composition of claim 2 , wherein the hydrogel composition includes sodium hyaluronate.

5. The composition of claim 4 included in a vessel.

6. The composition of claim 1 included in a vessel.

7. The composition of claim 1 , wherein the hydrogel composition includes at least one crystal.

8. The composition of claim 1 , wherein the hydrogel composition consists essentially of the uncrosslinked hyaluronic acid, the uncrosslinked alginate, the cell adhesive protein, and water.

9. The composition of claim 8 , wherein:
the uncrosslinked alginate includes glucuronic acid;
based on the uncrosslinked alginate including the glucuronic acid, the uncrosslinked alginate is configured to be crosslinked around the uncrosslinked hyaluronic acid with the ion.

10. A composition comprising:
a hydrogel composition;
wherein the hydrogel composition includes: (a) uncrosslinked hyaluronic acid; (b) uncrosslinked alginate located around the uncrosslinked hyaluronic acid; and (c) a biomineral;
wherein the uncrosslinked alginate is configured to be crosslinked around the uncrosslinked hyaluronic acid with an ion;
wherein the uncrosslinked hyaluronic acid is not configured to be crosslinked with the ion.

11. The composition of claim 10 , wherein the ion is calcium.

12. The composition of claim 11 , wherein the uncrosslinked alginate includes sodium alginate.

13. The composition of claim 11 , wherein the hydrogel composition includes sodium hyaluronate.

14. The composition of claim 13 included in a vessel.

15. The composition of claim 10 included in a vessel.

16. The composition of claim 10 , wherein the hydrogel composition includes at least one crystal.

17. The composition of claim 10 , wherein the hydrogel composition consists essentially of the uncrosslinked hyaluronic acid, the uncrosslinked alginate, the biomineral, and water.

18. The composition of claim 17 , wherein:
the uncrosslinked alginate includes glucuronic acid;
based on the uncrosslinked alginate including the glucuronic acid, the uncrosslinked alginate is configured to be crosslinked around the uncrosslinked hyaluronic acid with the ion.

Assignees

• Univ Texas

Inventors

• Zawko Scott
• Schmidt Christine

Classifications

• C08B37/0072 Primary

  Hyaluronic acid, i.e. HA or hyaluronan; Derivatives thereof, e.g. crosslinked hyaluronic acid (hylan) or hyaluronates · CPC title

• C08J9/26 Primary

  by elimination of a solid phase from a macromolecular composition or article, e.g. leaching out · CPC title

• C08B37/0084

  Guluromannuronans, e.g. alginic acid, i.e. D-mannuronic acid and D-guluronic acid units linked with alternating alpha- and beta-1,4-glycosidic bonds; Derivatives thereof, e.g. alginates · CPC title

• C08J3/075

  Macromolecular gels · CPC title

• C08J3/24

  Crosslinking, e.g. vulcanising, of macromolecules (mechanical aspects B29C35/00; crosslinking agents C08K) · CPC title