GRP78-derived peptide for identifying high-efficiency stem cells

Patent metadata

Field	Value
Publication number	US-11760792-B2
Application number	US-201816755373-A
Country	US
Kind code	B2
Filing date	Oct 15, 2018
Priority date	Oct 13, 2017
Publication date	Sep 19, 2023
Grant date	Sep 19, 2023

Abstract

The present invention relates to a GRP78-derived peptide for screening highly efficient stem cells and the use thereof, and more particularly, to screening highly efficient stem cells using, as a marker, a GRP78-derived peptide capable of binding to the binding domain of GRP78 protein on the cell surface. According to the present invention, the GRP78-derived peptide comprising only a specific amino acid sequence capable of recognizing highly efficient stem cells, among the amino acid sequence of GRP78, makes it possible to screen only non-senescent young stem cells. In addition, when stem cells are treated with the GRP78-derived peptide or the GRP78-derived peptide is introduced into stem cells, the efficiency of the stem cells can be increased. Thus, the GRP78-derived peptide is useful for the production of stem cell therapy products having excellent efficacy.

First claim

The invention claimed is:

1. A peptide consisting of the amino acid sequence of SEQ ID NO: 14.

2. The peptide of claim 1 , wherein the peptide is derived from Glucose-regulated protein 78 (GRP78).

3. The peptide of claim 1 , wherein the peptide of SEQ ID NO: 14 binds ATP, which is regulated by GRP78.

4. A method of screening highly efficient stem cells that express GRP78, comprising steps of:
(a) conjugating a fluorescent label to a peptide that binds GRP78 and consists of the amino acid sequence of SEQ ID NO: 14;
(b) culturing the fluorescent labeled-conjugated peptide with stem cells to allow binding of the fluorescent labeled-conjugated peptide with stem cells that express GRP78; and
(c) screening for GRP78-expressing stem cells under a fluorescence microscope.

5. A composition comprising the peptide of claim 1 .

6. A kit comprising the peptide of claim 1 .

7. A method of increasing efficiency of stem cells that express GRP78 comprising treating stem cells that express GRP78 with the peptide of claim 1 .

Assignees

• Nibec Co Ltd
• Nat Univ Chungbuk Ind Acad Coop Found
• Seoul Nat Univ R&Db Foundation

Inventors

• Park Yoon Shin
• Park Yoon Jeong
• Chung Chong-Pyoung
• Lee Jue-Yeon

Classifications

• G01N33/56966 Primary

  Animal cells · CPC title

• C07K16/18 Primary

  against material from animals or humans · CPC title

• G01N33/5073

  Stem cells · CPC title

• G01N33/68

  involving proteins, peptides or amino acids {(involving lipoproteins G01N33/92)} · CPC title

• G01N2560/00

  Chemical aspects of mass spectrometric analysis of biological material · CPC title