Human and non-human animal use of microbial anaplerotic oil

Patent metadata

Field	Value
Publication number	US-11758934-B2
Application number	US-202217818772-A
Country	US
Kind code	B2
Filing date	Aug 10, 2022
Priority date	Dec 22, 2017
Publication date	Sep 19, 2023
Grant date	Sep 19, 2023

Abstract

Disclosed are techniques and systems for producing microbials having anaplerotic oils that are rich in odd-chain fatty acids, and other beneficial components, at higher concentrations than those present in other natural dietary sources of OCFA, at lower cost, and higher production yield. Further, disclosed are examples of incorporation of these higher concentration OCFA products into food for human and non-human animal consumption.

First claim

What is claimed is:

1. A food additive comprising anaplerotic oil derived from a microorganism cultured aerobically with propionate or a precursor thereof, wherein the anaplerotic oil comprises saturated tridecanoic (C13:0), pentadecanoic (C15:0), and heptadecanoic (C17:0) odd-chain fatty acids (OCFA), wherein:
at least 5% of the anaplerotic oil from the microorganism's total fatty acid (TFA) content is saturated tridecanoic (C13:0), pentadecanoic (C15:0), and heptadecanoic (C17:0) OCFA; and
at least 2.2% cell dry weight (CDW) of the biomass from the microorganism is saturated tridecanoic (C13:0), pentadecanoic (C15:0), and heptadecanoic (C17:0) OCFA.

2. The food additive of claim 1 , wherein the microorganism is cultured aerobically with propionate.

3. The food additive of claim 2 , wherein pentadecanoic acid makes up greater than 18% of the TFA content of the anaplerotic oil and/or heptadecanoic acid makes up greater than 5% of the TFA content of the anaplerotic oil.

4. The food additive of claim 2 , wherein saturated tridecanoic (C13:0), pentadecanoic (C15:0), and heptadecanoic (C17:0) OCFA comprise a range of 5% to 60% of the TFA content of the anaplerotic oil.

5. The food additive of claim 2 , wherein the microorganism is a species from the class Labyrinthulomycetes.

6. The food additive of claim 5 , wherein microorganism is a thraustochytrid.

7. A food for consumption by human and non-human animals, said food comprising anaplerotic oil derived from a microorganism cultured aerobically with propionate or a precursor thereof, wherein the anaplerotic oil comprises saturated tridecanoic (C13:0), pentadecanoic (C15:0), and heptadecanoic (C17:0) odd-chain fatty acids (OCFA).

8. The food of claim 7 , wherein the microorganism is cultured aerobically with propionate.

9. The food of claim 8 , wherein:
the food is a dairy product; and
the % total fatty acid (% TFA) of saturated tridecanoic (C13:0), pentadecanoic (C15:0), and heptadecanoic (C17:0) OCFA is at least 5.5% greater than the % TFA of saturated tridecanoic (C13:0), pentadecanoic (C15:0), and heptadecanoic (C17:0) OCFA in a substantially identical dairy product that does not contain the anaplerotic oil.

10. The food of claim 8 , wherein the microorganism is a species from the class Labyrinthulomycetes.

11. The food of claim 10 , wherein microorganism is a thraustochytrid.

12. A processed food product or food component comprising a food additive, the food additive comprising anaplerotic oil derived from a microorganism cultured aerobically with propionate or a precursor thereof, wherein the anaplerotic oil comprises saturated tridecanoic (C13:0), pentadecanoic (C15:0), and heptadecanoic (C17:0) odd-chain fatty acids (OCFA).

13. The processed food product or food component of claim 12 , wherein the microorganism is cultured aerobically with added propionate.

14. The processed food product or food component of claim 13 , wherein:
the processed food product or food component is a dairy product; and
the % total fatty acid (% TFA) of saturated tridecanoic (C13:0), pentadecanoic (C15:0), and heptadecanoic (C17:0) OCFA is at least 5.5% greater than the % TFA of saturated tridecanoic (C13:0), pentadecanoic (C15:0), and heptadecanoic (C17:0) OCFA in a substantially identical dairy product that does not contain the anaplerotic oil.

15. The processed food product or food component of claim 13 , wherein the microorganism is a species from the class Labyrinthulomycetes.

16. The processed food product or food component of claim 15 , wherein microorganism is a thraustochytrid.

17. The food additive of claim 1 , wherein:
at least 2.7% CDW of the biomass from the microorganism is saturated tridecanoic (C13:0), pentadecanoic (C15:0), and heptadecanoic (C17:0) OCFA.

18. The food additive of claim 1 , wherein:
at least 14.7% CDW of the biomass from the microorganism is saturated tridecanoic (C13:0), pentadecanoic (C15:0), and heptadecanoic (C17:0) OCFA.

19. The food additive of claim 1 , wherein:
at least 25% CDW of the biomass from the microorganism is saturated tridecanoic (C13:0), pentadecanoic (C15:0), and heptadecanoic (C17:0) OCFA.

20. The food additive of claim 1 , wherein: up to 39.25% CDW of the biomass from the microorganism is saturated tridecanoic (C13:0), pentadecanoic (C15:0), and heptadecanoic (C17:0) OCFA.

Assignees

• Heliae Dev Llc

Inventors

• Lamont Micheal
• Amezquita Ernult Magdalena
• Ganuza Taberna Eneko

Classifications

• A23L29/04 Primary

  Fatty acids or derivatives · CPC title

• C12P7/6409

  Fatty acids · CPC title

• A23C9/1315

  Non-milk proteins or fats; Seeds, pulses, cereals or soja; Fatty acids, phospholipids, mono- or diglycerides or derivatives therefrom; Egg products · CPC title

• C12P7/6463

  obtained from glyceride producing microorganisms, e.g. single cell oil · CPC title

• A23C9/1528

  Fatty acids; Mono- or diglycerides; Petroleum jelly; Paraffine; Phospholipids; Derivatives thereof · CPC title