Compositions and methods for laser capture microdissection

Patent metadata

Field	Value
Publication number	US-11747242-B2
Application number	US-202016878926-A
Country	US
Kind code	B2
Filing date	May 20, 2020
Priority date	Dec 22, 2015
Publication date	Sep 5, 2023
Grant date	Sep 5, 2023

Abstract

Compositions and methods for the simultaneous capture and release using micropattern surfaces for tissue and cell microdissection. In one example, a patterned thermoplastic film has a first surface and a plurality of projections attached to and extending outwardly from the first surface. The projections form a pattern on the thermoplastic film.

First claim

What is claimed is:

1. A kit comprising:
a micropatterned thermoplastic film comprising
a first surface; and
a plurality of projections secured to the first surface and extending outwardly therefrom relative to an axis that is substantially perpendicular to the first surface, wherein at least one projection of the plurality of projections comprises a tip coated with one or more nanoparticles or one or more microparticles, and wherein the one or more nanoparticles or the one or more microparticles comprise reversible cross-links configured to cleave when the at least one projection is irradiated with electromagnetic radiation in the UV spectrum,
the at least one projection of the plurality of projections being a microsphere or microneedle that is activable by applying irradiation thereto.

2. The kit of claim 1 , wherein the plurality of projections are attached to the first surface.

3. The kit of claim 1 , wherein the plurality of projections are continuous with the first surface.

4. The kit of claim 1 , wherein the plurality of projections are integrally formed with the first surface.

5. The kit of claim 1 , wherein the plurality of projections are disposed as an array on the first surface.

6. The kit of claim 1 , further comprising hydrogel nanoparticles provided on the plurality of projections.

7. The kit of claim 1 , wherein the at least one projection has a constant diameter.

8. The kit of claim 1 , wherein the at least one projection is a microneedle of variable diameter that decreases outwardly from the first surface.

9. The kit of claim 1 , wherein the at least one projection is the microsphere comprising hydrogel.

10. The kit of claim 1 , wherein the one or more nanoparticles or the one or more microparticles comprise dissociating cross-links configured to dissociate when the at least one projection is irradiated with electromagnetic radiation in the UV spectrum.

11. The kit of claim 1 , wherein the thermoplastic film further comprises an adsorptive substance.

12. The kit of claim 11 , wherein the adsorptive substance is tin (IV) 2,3-naphthalocyanine dichloride; silicon (IV) 2,3-naphthalocyanine dihydroxide; silicon (IV) 2,3-naphthalocyanine dioctyloxide; or vanadyl 2,11,20,29-tetra-tert-butyl-2,3-naphthalocyanine, or any combination thereof.

13. The kit of claim 1 , wherein the micropatterned thermoplastic film comprises an ethyl vinyl acetate polymer, a vipolyurethane polymer; a polyvinyl acetate polymer; an ethylene-methyl acrylate polymer; a polycarbonate polymer; an ethylene-vinyl alcohol copolymer; a polypropylene polymer; or an expandable or general purpose polystyrene polymer, or any combination thereof.

14. The kit of claim 1 , further comprising:
instructions for use of the micropatterned thermoplastic film to perform laser capture microdissection.

15. A kit comprising:
a micropatterned thermoplastic film comprising
a first surface; and
a plurality of projections secured to the first surface and extending outwardly therefrom relative to an axis that is substantially perpendicular to the first surface, wherein at least one projection comprises a tip coated with one or more nanoparticles or one or more microparticles, and wherein the one or more nanoparticles or the one or more microparticles comprise reversible cross-links configured to cleave when the at least one projection is irradiated with electromagnetic radiation in the IR spectrum,
the at least one projection of the plurality of projections being a microsphere or microneedle that is activable by applying irradiation thereto.

16. The kit of claim 15 , wherein the thermoplastic film further comprises an adsorptive substance.

17. The kit of claim 15 , wherein the one or more nanoparticles or the one or more microparticles comprise dissociating cross-links configured to dissociate when the at least one projection is irradiated with electromagnetic radiation in the IR spectrum.

18. A kit comprising:
a micropatterned thermoplastic film comprising
a first surface; and
a plurality of projections secured to the first surface and extending outwardly therefrom relative to an axis that is substantially perpendicular to the first surface, wherein the at least one projection comprises a tip coated with one or more nanoparticles or one or more microparticles, and wherein the one or more nanoparticles or the one or more microparticles comprises an outer shell and an inner core,
the at least one projection of the plurality of projections being a microsphere or microneedle that is activable by applying irradiation thereto.

19. The kit of claim 18 , wherein the inner core is configured to adhere to tissue or a cell component in contact with the micropatterned thermoplastic film, and wherein the outer shell is configured not to adhere to the tissue or cell component.

20. The kit of claim 19 , wherein the one or more nanoparticles or the one or more microparticles is configured to adhere to the tissue or cell component in the absence of irradiation with electromagnetic radiation and is configured not to adhere to the tissue or cell component upon irradiation with electromagnetic radiation.

Assignees

• George Mason Res Foundation Inc

Inventors

• Liotta Lance
• Espina Virginia
• Agrawal Nitin
• Kunkel Alessandra Luchini

Classifications

• G01N1/2813 Primary

  Producing thin layers of samples on a substrate, e.g. smearing, spinning-on (G01N1/30 takes precedence) · CPC title

• G01N2001/284

  using local activation of adhesive, i.e. Laser Capture Microdissection · CPC title