Methods for Accelerated and Enhanced Cardiac Differentiation of IPS Cells by Electrical Stimulation
US-2016201035-A1 · Jul 14, 2016 · US
Methods and systems for functional maturation of iPSC and ESC derived cardiomyocytes
US11746328B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11746328-B2 |
| Application number | US-201816490302-A |
| Country | US |
| Kind code | B2 |
| Filing date | Mar 3, 2018 |
| Priority date | Mar 3, 2017 |
| Publication date | Sep 5, 2023 |
| Grant date | Sep 5, 2023 |
How to read this patent
A practical reading order for non-experts. Skip the full description unless you need deep technical detail.
- Title
What the patent document calls the invention.
- Abstract
A short plain-language summary of the technical disclosure.
- Assignees and inventors
Who owns or filed the patent and who is credited as inventor.
- Key dates
Filing, priority, publication, and grant dates set the timeline.
- First independent claim
The legal scope of protection — read this for what is actually claimed.
- CPC / IPC classifications
Technology tags used to group this patent with similar filings.
- Citations and related patents
Prior art links and similar publications in this corpus.
Abstract
Official abstract text for this publication.
Methods of inducing functional maturation of immature cardiomyocytes derived from induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs) by electrically pacing the immature cardiomyocytes according to a pulse profile that induces maturation until the immature cardiomyocytes mature into functionally adult cardiomyocytes.
First claim
Opening claim text (preview).
What is claimed is: 1. A method of maturing functionally immature cardiomyocytes, the method comprising: a) providing a system configured to culture, electrically pace, and monitor beating of beating cells; b) culturing immature cardiomyocytes in the system; c) determining whether or not beating of the immature cardiomyocytes is synchronized by electrically monitoring the immature cardiomyocytes; d) if the beating is not synchronized, electrically pacing the immature cardiomyocytes until the cardiomyocyte beating is synchronized; and e) if the beating is synchronized, electrically pacing the immature cardiomyocytes according to a pulse profile that induces maturation until the immature cardiomyocytes mature into functionally adult cardiomyocytes; wherein the electric pacing in step 1d) is at a different pulse frequency than the electric pacing of step 1e). 2. The method of claim 1 , wherein the electric monitoring of the immature cardiomyocytes is by a method selected from the group consisting of monitoring cell-substrate impedance of the immature cardiomyocytes, performing extracellular recording of the immature cardiomyocytes, and both monitoring cell-substrate impedance and performing extracellular recording of the immature cardiomyocytes. 3. The method of claim 1 , wherein the pulse profile comprises one or more characteristics selected from the group consisting of a rectangular pulse shape, a pulse intensity of 0.7 V to 1 V, a pulse duration from 0.1 milliseconds to 0.2 milliseconds, and a pulse frequency between 1 Hz and 2 Hz. 4. The method of claim 1 , wherein the pulse profile comprises an increasing pulse frequency over time, optionally from 0.75 Hz to 2 Hz. 5. The method of claim 1 , wherein the immature cardiomyocytes are paced until displaying a positive beating force-frequency relationship in step e).
Assignees
Inventors
Classifications
Culture process characterised by the use of electromagnetic stimulation · CPC title
from artificially induced pluripotent stem cells · CPC title
Circuits therefor (measuring impedance per se G01R27/02) · CPC title
Artificially induced pluripotent stem cells, e.g. iPS · CPC title
- C12N5/0657Primary
Cardiomyocytes; Heart cells · CPC title
Patent family
Related publications grouped by family.
External sources
Frequently asked questions
Answers are generated from the same data shown on this page.
- What does patent US11746328B2 cover?
- Methods of inducing functional maturation of immature cardiomyocytes derived from induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs) by electrically pacing the immature cardiomyocytes according to a pulse profile that induces maturation until the immature cardiomyocytes mature into functionally adult cardiomyocytes.
- Who is the assignee on this patent?
- Agilent Technologies Inc
- What technology area does this patent fall under?
- Primary CPC classification C12N5/0657. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Sep 05 2023 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 3 related publications on this page (citations in our corpus or others sharing the same primary CPC).