Process for preparing a pooled human platelet lysate, pooled human platelet lysate and its use for treating neurological disorders

Patent metadata

Field	Value
Publication number	US-11744863-B2
Application number	US-201816622446-A
Country	US
Kind code	B2
Filing date	Jun 15, 2018
Priority date	Jun 16, 2017
Publication date	Sep 5, 2023
Grant date	Sep 5, 2023

Abstract

Process for preparing a heat-treated pooled human platelet lysate, said process comprising the steps of: a) Providing a pooled human platelet lysate (p HPL), b) Heat-treating the pooled human platelet lysate at a temperature of 50° C. to 70° C. during 20 to 40 minutes, c) Purifying the heat-treated pooled human platelet lysate of step b).

First claim

The invention claimed is:

1. A method for treatment of a neurological disorder, comprising the step of administering a heat-treated pooled human platelet lysate to a patient or subject in need thereof, wherein the heat-treated pooled human platelet lysate has a fibrinogen content of less than 5% by weight of the fibrinogen content of non-heat-treated pHPL and a fibrinogen content of less than 50 ng/mL.

2. The method according to claim 1 , wherein said heat-treated pooled human platelet lysate has a fibrinogen content of less than 3% by weight of the fibrinogen content of non-heat-treated pHPL and a fibrinogen content of less than 30 ng/mL.

3. The method according to claim 1 , wherein said heat-treated pooled human platelet lysate has a fibrinogen content of less than 1% by weight of the fibrinogen content of non-heat-treated pHPL and a fibrinogen content of less than 15 ng/mL.

4. The method according to claim 1 , wherein the neurological disorder is selected from neurodegenerative disorders, neuro inflammatory disorders, neurodevelopment disorders, neurovascular disorders and cerebral insults.

5. The method according to claim 4 , wherein the neurological disorder is a neurodegenerative disorder selected from multiple sclerosis (MS), Parkinson's disease (PD), Huntington's disease (HD), Amyotrophic lateral sclerosis (ALS), stroke, age-related macular degeneration (AMD), Alzheimer's disease (AD), vascular dementia, frontotemporal dementia, semantic dementia and dementia with Lewy bodies.

6. The method according to claim 5 , wherein the neurodegenerative disorder is selected from Parkinson's disease, amyotrophic lateral sclerosis, age-related macular degeneration and Alzheimer's disease.

7. The method according to claim 4 , wherein the neurological disorder is a cerebral insult selected from hypoxia or traumatic brain injury.

8. The method according to claim 1 , wherein said heat-treated pooled human platelet lysate is administrated by intrathecal, intraocular, intranasal or intra cerebroventricular route.

9. The method according to claim 8 , wherein said heat-treated pooled human platelet lysate is administrated by intra cerebroventricular route.

10. The method according to claim 9 , wherein said heat-treated pooled human platelet lysate is adapted to be administered with a programmable medication pump.

11. The method according to claim 8 , wherein said heat-treated pooled human platelet lysate is administrated into the right lateral ventricle.

12. The method according to claim 8 , wherein said heat-treated pooled human platelet lysate is administrated closed to the intraventricular foramen.

13. The method according to claim 8 , wherein said heat-treated pooled human platelet lysate is administrated into the third ventricle.

Assignees

• Centre Hospitalier Regional Et Univ De Lille Chru
• Univ Lille
• Univ Du Littoral Cote Dopale
• Inst Nat Sante Rech Med
• Univ Taipei Medical
• Univ Lille Ii Droit & Sante
• Inst Nat Sante Rech Med

Inventors

• Devos David
• Burnouf Thierry
• Devedjian Jean-Christophe
• Chou Ming-Li
• Gouel Flore

Classifications

• A61K35/19 Primary

  Platelets; Megacaryocytes · CPC title

• C12N5/0644

  Platelets; Megakaryocytes · CPC title

• A61P25/28

  for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia · CPC title

• A61K9/0085

  Brain, e.g. brain implants; Spinal cord · CPC title