Insecticidal proteins from plants and methods for their use

Patent metadata

Field	Value
Publication number	US-11739344-B2
Application number	US-201816619984-A
Country	US
Kind code	B2
Filing date	Jun 14, 2018
Priority date	Jun 16, 2017
Publication date	Aug 29, 2023
Grant date	Aug 29, 2023

Abstract

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

First claim

That which is claimed:

1. An anti-Coleopteran polypeptide comprising an amino acid sequence having at least 80% sequence identity compared to the amino acid sequence of SEQ ID NO: 102.

2. The anti-Coleopteran polypeptide of claim 1 , wherein the anti-Coleopteran polypeptide is operably linked to a heterologous transit peptide or heterologous signal polypeptide.

3. An anti-Coleopteran composition comprising the anti-Coleopteran polypeptide of claim 1 .

4. A recombinant polynucleotide encoding an anti-Coleopteran polypeptide comprising an amino acid sequence having at least 80% sequence identity compared to the amino acid sequence of SEQ ID NO: 102.

5. The recombinant polynucleotide of claim 4 , wherein the polynucleotide is a cDNA.

6. A DNA construct comprising the recombinant polynucleotide of claim 4 and a heterologous regulatory sequence operably linked to the recombinant polynucleotide.

7. A transgenic plant or plant cell comprising the DNA construct of claim 6 .

8. A method of inhibiting growth or killing a Coleopteran population comprising contacting the Coleopteran population with an insecticidally-effective amount of the anti-Coleopteran polypeptide of claim 1 .

9. A method of controlling Coleopteran damage to plants comprising providing a plant part of the plant of claim 7 to a Coleopteran pest or Coleopteran pest population for ingestion.

10. The method of claim 8 , wherein the Coleopteran pest or Coleopteran pest population is resistant to a Bt toxin.

Assignees

• Pioneer Hi Bred Int

Inventors

• Barry Jennifer Kara
• D'Oliviera Angel Elizabeth Grace
• Gerber Ryan Michael
• Hayes Kevin
• Lu Albert L
• Lum Amy
• Schellenberger Ute
• Schepers Eric
• Sopa Jeffrey
• Xie Weiping
• Yalpani Nasser

Classifications

• C07K14/325

  Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins · CPC title

• C12N15/8286 Primary

  for insect resistance · CPC title

• A01N63/50

  Isolated enzymes; Isolated proteins (peptides A01N37/46) · CPC title

• C07K14/415

  from plants · CPC title

• C12N15/111

  General methods applicable to biologically active non-coding nucleic acids · CPC title