Engineered skin equivalent, method of manufacture thereof and products derived therefrom

Patent metadata

Field	Value
Publication number	US-11739217-B2
Application number	US-202318092816-A
Country	US
Kind code	B2
Filing date	Jan 3, 2023
Priority date	Apr 21, 2016
Publication date	Aug 29, 2023
Grant date	Aug 29, 2023

Abstract

Disclosed herein are synthetic leathers, artificial epidermal layers, artificial dermal layers, layered structures, products produced therefrom and methods of producing the same.

First claim

What is claimed is:

1. A method of forming a tanned synthetic leather, wherein the method comprises:
a) forming an artificial dermal layer comprising a fibroblast on a scaffold, wherein the scaffold comprises cellulose or a modified version thereof;
b) processing the artificial dermal layer to provide an extracellular matrix; and
c) tanning at least a portion of the extracellular matrix to generate the tanned synthetic leather.

2. The method of claim 1 , wherein the cellulose or a modified version thereof comprises rayon.

3. The method of claim 1 , wherein a thickness of the artificial dermal layer ranges from about 0.02 mm to about 5 mm.

4. The method of claim 1 , wherein the processing comprises a member selected from the group consisting of: a preserving, a soaking, a bating, a pickling, a depickling, a thinning, a retanning, a lubricating, a crusting, a wetting, a sammying, a shaving, a rechroming, a neutralizing, a dyeing, a fatliquoring, a filling, a stripping, a stuffing, a whitening, a fixating, a setting, a drying, a conditioning, a milling, a staking, a buffing, a finishing, an oiling, a brushing, a padding, an impregnating, a spraying, a roller coating, a curtain coating, a polishing, a plating, an embossing, an ironing, a glazing, a tumbling, and any combination thereof.

5. The method of claim 1 , wherein the processing comprises removing at least a portion of the artificial dermal layer to provide the extracellular matrix.

6. The method of claim 1 , wherein the extracellular matrix comprises collagen.

7. The method of claim 6 , wherein the collagen comprises a type I collagen, a type III collagen, or a combination thereof.

8. The method of claim 1 , wherein the tanning comprises a vegetable tanning, a chrome tanning, an aldehyde tanning, a syntan tanning, a bacterial dyeing, or any combination thereof.

9. The method of claim 1 , further comprising forming the tanned synthetic leather into one or more of a watch strap, a belt, a packaging, a shoe, a boot, a footwear, a glove, a clothing, a luggage, a bag, a clutch, a purse, a backpack, a wallet, a saddle, a harness, a whip, or any combination thereof.

10. The method of claim 1 , wherein the artificial dermal layer comprises cultured mammalian cells.

11. A method of forming a tanned synthetic leather, wherein the method comprises:
a) forming a cell layer comprising a fibroblast on a scaffold, wherein the scaffold comprises cellulose or a modified version thereof;
b) wherein the cell layer comprises collagen; and
c) tanning at least a portion of the collagen to generate the tanned synthetic leather.

12. The method of claim 11 , wherein the cell layer comprises a dermal cell layer.

13. The method of claim 11 , wherein the cellulose or a modified version thereof comprises rayon.

14. The method of claim 11 , wherein the collagen comprises a type I collagen, a type III collagen, or a combination thereof.

15. The method of claim 11 , wherein the cell layer is cultured in vitro.

16. The method of claim 15 , wherein the cell layer comprises mammalian cells.

17. The method of claim 11 , wherein tanning comprises a vegetable tanning, a chrome tanning, an aldehyde tanning, a syntan tanning, a bacterial dyeing, or any combination thereof.

18. The method of claim 11 , further comprising forming the tanned synthetic leather into one or more of a watch strap, a belt, a packaging, a shoe, a boot, a footwear, a glove, a clothing, a luggage, a bag, a clutch, a purse, a backpack, a wallet, a saddle, a harness, a whip, or any combination thereof.

Assignees

• Vitrolabs Inc
• King S College London

Inventors

• Helgason Ingvar
• Ilic Dusko

Classifications

• C12N5/0698 Primary

  Skin equivalents · CPC title

• C12N5/0656 Primary

  Adult fibroblasts · CPC title

• C12N2533/54

  Collagen; Gelatin · CPC title

• C08L89/06 Primary

  derived from leather or skin {, e.g. gelatin} · CPC title

• C12N5/0625

  Epidermal cells, skin cells; Cells of the oral mucosa · CPC title