Systems and method for sorting particles suspended in a fluid

Patent metadata

Field	Value
Publication number	US-11738352-B1
Application number	US-202217741625-A
Country	US
Kind code	B1
Filing date	May 11, 2022
Priority date	May 11, 2022
Publication date	Aug 29, 2023
Grant date	Aug 29, 2023

Abstract

A system and method sorts particles suspended in a fluid. The system includes a transverse flow filter and a microfluidic sorter. The transverse flow filter concentrates the particles in a retained portion of the fluid. The microfluidic sorter sorts the particles and includes a delivery channel and at least one sorting stage. The delivery channel is dimensioned to permit passage of all the particles in the retained fluid from the transverse flow filter. The delivery channel delivers the particles in the retained fluid to a respective input for each sorting stage and exhausts a remainder of both the particles and the retained fluid at a drain output. Each sorting stage has a labyrinth of interconnected passageways dimensioned to permit passage from the respective input to a respective output for the sorting stage of those of the particles smaller than a respective size for the sorting stage.

First claim

We claim:

1. A system for sorting a plurality of particles suspended in a fluid, comprising:
a transverse flow filter for concentrating the particles in a retained portion of the fluid; and
a microfluidic sorter for sorting the particles, the microfluidic sorter including:
a delivery channel dimensioned to permit passage of all the particles in the retained fluid from the transverse flow filter, the delivery channel delivering the particles in the retained fluid to a respective input for each of at least one sorting stage and exhausting a remainder of both the particles and the retained fluid at a drain output; and
the at least one sorting stage, which each have a labyrinth of interconnected passageways dimensioned to permit passage from the respective input to a respective output for the sorting stage of those of the particles smaller than a respective size for the sorting stage.

2. The system of claim 1 , wherein the transverse flow filter includes a transfer channel having a surrounding wall with a plurality of pores dimensioned to permit passage of the fluid and not the particles, a retentate including the particles and that of the fluid passing through the transfer channel and a permeate including that of the fluid passing through the pores, the retentate concentrating the particles in the retained portion of the fluid.

3. The system of claim 2 , wherein the retentate is repeatedly recycled through the transverse flow filter during a concentrating loop until a quantity of the retained portion of the fluid reduces to a predetermined quantity.

4. The system of claim 3 , further comprising a valve that, upon the retained portion of the fluid becoming reduced to the predetermined quantity, switches a flow of the particles and the retained fluid away from the transverse flow filter for concentrating the particles during the concentrating loop and towards the microfluidic sorter for sorting the particles during a sorting cycle.

5. The system of claim 1 for sorting and additionally interrogating the particles, the system further comprising a respective sensor for each of the at least one sorting stage, the respective sensor for interrogating those of the particles at the respective output for the sorting stage.

6. The system of claim 5 , wherein:
the fluid is seawater;
the particles include microbial organisms suspended in the seawater;
the respective output for each sorting stage has a diameter matching a spot size of a laser; and
the respective sensor for each sorting stage includes the laser interrogating the microbial organisms across the spot size of the respective output for the sorting stage.

7. The system of claim 6 , wherein the at least one sorting stage is a plurality of sorting stages in a sequence from an initial sorting stage to a last sorting stage, and the laser for the initial sorting stage interrogates the microbial organisms smaller than the respective size for the initial sorting stage and a laser for the last sorting stage interrogates the microbial organisms smaller than the respective size for the last sorting stage, with the respective size for the initial sorting stage smaller than the respective size for the last sorting stage.

8. The system of claim 1 , wherein the at least one sorting stage is a plurality of sorting stages in a sequence from an initial sorting stage to a last sorting stage, with the respective size for each the sorting stages monotonically increasing from the initial sorting stage to the last sorting stage, such that the respective size for the initial sorting stage is smaller than the respective size for the last sorting stage.

9. The system of claim 8 , wherein:
the delivery channel of the microfluidic sorter delivers the particles in the retained fluid to the sorting stages in an order corresponding to the sequence beginning with the initial sorting stage and ending with the last sorting stage, such that the respective input for each of the sorting stages receives a diminished concentration of the particles smaller than the respective size for any preceding one or ones of the sorting stages in the sequence; and
the delivery channel of the microfluidic sorter delivers to the respective input for the last sorting stage before the delivery channel of the microfluidic sorter exhausts the remainder at the drain output.

10. The system of claim 9 , wherein the transverse flow filter includes a transfer channel having a surrounding wall with a plurality of pores dimensioned smaller than the respective size for each of the sorting stages to permit passage of the fluid and not the particles, a retentate including the particles and that of the fluid passing through the transfer channel and a permeate including that of the fluid passing through the pores, the retentate concentrating the particles in the retained portion of the fluid.

11. The system of claim 1 , further comprising a pump for pumping the retained fluid with the particles through the delivery channel of the microfluidic sorter to the respective output for each of the at least one sorting stage and to the drain output, the pump selected from a group consisting of a peristaltic pump and a syringe.

12. The system of claim 1 , further comprising a dead-end filter for blocking any additional particles in the fluid that are too large for passage through the delivery channel of the microfluidic sorter.

13. The system of claim 1 , wherein the microfluidic sorter includes a plurality of pillars in each of the at least one sorting stage, a space between the pillars providing the labyrinth of interconnected passageways dimensioned to permit passage of those of the particles smaller than the respective size for the sorting stage.

14. The system of claim 13 , wherein the pillars of each sorting stage are arranged in a hexagonal grid with a width of nearest approach between each adjacent pair of the pillars of each sorting stage matching the respective size for the sorting stage and a height of the pillars of each sorting stage also matching the respective size for the sorting stage.

15. The system of claim 14 , wherein the width of nearest approach between each adjacent pair of the pillars of each sorting stage is at least an order of magnitude larger than a width of the pillars of the sorting stage.

16. The system of claim 14 , wherein the microfluidic sorter includes a gap between the delivery channel and the at least one sorting stage, the gap also having a height matching the respective size for each sorting stage.

17. The system of claim 14 , wherein the microfluidic sorter includes a substrate having a major surface providing an interior surface of the delivery channel and the labyrinth of interconnected passageways of the at least one sorting stage, the pillars of each sorting stage molded from polydimethylsiloxane (PDMS) with a cap of each of the pillars of each sorting stage in contact with the major surface of the substrate.

18. The system of claim 17 , wherein:
the at least one sorting stage is a plurality of sorting stages in a sequence from an initial sorting stage to a last sorting stage, with the respective size for each the sorting stages monotonically increasing from the initial sorting stage to the last sorting stage, such that the respective size for the initial sorting stage is smaller than the respective size for the last sorting stage;
the initial sorting stage has the width of nearest approach between each adjacent pair of the pillars of the initial sorting stage, the height of the pillars of the initial sorting stage, and a gap between the delivery

Assignees

• Us Navy
• Us Navy

Inventors

• Sims Patrick Craig
• Emery-Adleman Teresa
• Sabater Andrew B
• George Robert D
• Sorensen Kara C
• Obraztsova Anna
• Boss Pamela A

Classifications

• B01L3/502753 Primary

  characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation · CPC title

• B01L2200/0652

  Sorting or classification of particles or molecules · CPC title

• B01L2300/0864

  comprising only one inlet and multiple receiving wells, e.g. for separation, splitting · CPC title

• B01L2400/0487

  fluid pressure, pneumatics · CPC title

• B01L2300/0681

  Filter · CPC title