Method for quickly and accurately analyzing polyphenol content in rapeseed oil

Patent metadata

Field	Value
Publication number	US-11733230-B2
Application number	US-202017139084-A
Country	US
Kind code	B2
Filing date	Dec 31, 2020
Priority date	Mar 14, 2019
Publication date	Aug 22, 2023
Grant date	Aug 22, 2023

Abstract

The disclosure discloses a method for quickly and accurately analyzing polyphenol content in rapeseed oil, and belongs to the field of analysis of natural compounds. The separation method of the disclosure uses acetonitrile-water as an extractant to extract polyphenols from the rapeseed oil, and cooperates with a C 18 adsorbent for purification, and then performs separation and purification. Compared with the traditional liquid-liquid extraction and solid-phase extraction, the method has an average recovery rate of polyphenols in the rapeseed oil of 81.31% to 102.95%, and RSDs of 0.86% to 8.03%, and has higher accuracy and precision. The method of the disclosure not only uses less organic reagents and causes less environmental pollution, but also reduces matrix interference and improves purification efficiency through optimization of the adsorbent. The method of the disclosure not only is simple to operate and low in cost, but also has less matrix interference and accurate results, and is suitable for the qualitative and quantitative determination of polyphenols in the rapeseed oil.

First claim

What is claimed is:

1. A method for extracting polyphenols from rapeseed oil, comprising:
(1) extracting the rapeseed oil by using a mixed solution of acetonitrile and water as an extractant, performing solid-liquid separation, and collecting a supernatant to obtain an extracting solution; and
(2) adding 20 to 35 mg/mL C 18 adsorbent to the extracting solution obtained in step (1), performing solid-liquid separation, and collecting a supernatant to obtain a polyphenol solution.

2. The method according to claim 1 , wherein a volume ratio of acetonitrile to water in the extractant is (5 to 8):1.

3. The method according to claim 1 , wherein a mass-volume ratio of rapeseed oil to extractant in step (1) is 0.15 to 0.35 g/mL.

4. The method according to claim 1 , further comprising:
(1) extracting the rapeseed oil by using the mixed solution of acetonitrile and water as the extractant, and separating extract liquor to obtain the extracting solution, wherein a volume ratio of acetonitrile to water in the extractant is (5 to 8):1, and a mass-volume ratio of rapeseed oil to extractant is 0.15 to 0.35 g/mL; and
(2) adding 20 to 35 mg/mL C 18 adsorbent to the extracting solution obtained in step (1), and performing solid-liquid separation to obtain the polyphenol solution.

5. The method according to claim 1 , wherein the step (1) further comprises adding anhydrous MgSO 4 and NaCl to cooperate with the extractant.

6. The method according to claim 5 , wherein a mass ratio of anhydrous MgSO 4 to NaCl is 2:1 to 6:1.

7. The method according to claim 1 , wherein the step (1) further comprises performing a freezing treatment on separated liquid to obtain the extracting solution.

8. The method according to claim 4 , wherein the step (1) further comprises performing a freezing treatment on separated liquid to obtain the extracting solution.

9. The method according to claim 7 , wherein the freezing treatment is performed at a temperature of −15° C. to −20° C. for 1.5 to 2.5 h.

10. The method according to claim 8 , wherein the freezing treatment is performed at a temperature of −15° C. to −20° C. for 1.5 to 2.5 h.

11. The method according to claim 1 , wherein the step (2) further comprises adding anhydrous MgSO 4 to be used in combination with the adsorbent, so as to further remove moisture and fatty impurities in the extracting solution to obtain the polyphenol solution.

12. The method according to claim 4 , wherein the step (2) further comprises adding anhydrous MgSO 4 to be used in combination with the adsorbent, so as to further remove moisture and fatty impurities in the extracting solution to obtain the polyphenol solution.

13. A method for detecting polyphenols in vegetable oil, comprising pre-treating the vegetable oil to obtain a polyphenol solution by using the method according to claim 1 , and then performing detection.

14. The method according to claim 13 , wherein the detection is liquid chromatography detection.

Assignees

• Univ Jiangnan

Inventors

• Liu Yuanfa
• Song Junge
• Xu Yongjiang
• Zheng Zhaojun
• Cao Chen
• Zhai Yinghong

Classifications

• G01N33/03 Primary

  Edible oils or edible fats · CPC title

• B01D11/048

  Mixing by counter-current streams provoked by centrifugal force, in rotating coils or in other rotating spaces · CPC title

• B01D11/0492 Primary

  Applications, solvents used · CPC title

• G01N1/405

  by adsorption or absorption · CPC title

• G01N1/4022

  by thermal techniques; Phase changes · CPC title