Kit, device, and method for detecting bladder cancer

Patent metadata

Field	Value
Publication number	US-11732307-B2
Application number	US-201917050134-A
Country	US
Kind code	B2
Filing date	Apr 25, 2019
Priority date	Apr 25, 2018
Publication date	Aug 22, 2023
Grant date	Aug 22, 2023

Abstract

An embodiment according to the present invention provides a kit or device for detection of bladder cancer, and a method for detecting bladder cancer. An embodiment according to the present invention relates to: a kit or device for detection of bladder cancer, including a nucleic acid(s) capable of specifically binding to an miRNA(s) or a complementary strand(s) thereof in a sample from a subject; and a method for detecting bladder cancer, including measuring the miRNA(s) in vitro.

First claim

The invention claimed is:

1. A method for detecting bladder cancer, comprising:
measuring an expression level of miR-1185-1-3p in a sample from a human subject;
determining that the level of miR-1185-1-3p in the sample from the human subject is increased in comparison to a level of miR-1185-1-3p in a control sample from a human subject without bladder cancer;
determining that the human subject has bladder cancer based on the increased expression level of miR-1185-1-3p; and
treating the human subject for the bladder cancer;
wherein the sample is blood, serum, or plasma; and
wherein the treating comprises surgery, radiotherapy, chemotherapy, or a combination thereof.

2. The method according to claim 1 , comprising: plugging the gene expression level of miR-1185-1-3p in the sample from the subject into a discriminant formula capable of discriminating the presence or absence of a bladder cancer distinctively, wherein the discriminant formula is created by using gene expression levels in samples from subjects known to have bladder cancer and gene expression levels in samples from subjects having no bladder cancer as training samples.

3. The method according to claim 1 , comprising: measuring an expression level of miR-1185-1-3p by using a nucleic acid(s) capable of specifically binding to miR-1185-1-3p or the complement thereof, wherein the nucleic acid(s) is a polynucleotide(s) selected from the group consisting of:
(a) a polynucleotide consisting of SEQ ID NO: 2 or the complement thereof;
(b) a polynucleotide comprising SEQ ID NO: 2 or the complement thereof; and
(c) a polynucleotide derived from SEQ ID NO: 2, wherein the uracils have been replaced with thymines, or a complement thereof.

4. The method according to claim 1 , further comprising: measuring an expression level(s) of at least one polynucleotide selected from miR-1202, miR-1207-5p, miR-1246, miR-1254, miR-135a-3p, miR-1469, miR-149-3p, miR-150-3p, miR-1914-3p, miR-191-5p, miR-423-5p, miR-663a, miR-92a-2-5p, miR-92a-3p, and miR-940.

5. The method according to claim 4 , comprising: measuring an expression level of the at least one polynucleotide by using a nucleic acid(s) capable of specifically binding to the at least one polynucleotide or the complement thereof, wherein the nucleic acid(s) is a polynucleotide(s) selected from the group consisting of:
(a) a polynucleotide consisting of any of SEQ ID NOs: 229-243 or the complements thereof;
(b) a polynucleotide comprising any of SEQ ID NOs: 229-243 or the complements thereof; and
(c) a polynucleotide derived from any of SEQ ID NOs: 229-243, wherein the uracils have been replaced with thymines, or the complements thereof.

6. The method according to claim 1 , wherein the sample is serum or plasma.

7. The method according to claim 1 , wherein the method further comprises measuring an expression level(s) of at least one polynucleotide selected from miR-6087, miR-1185-2-3p, miR-1193, miR-1199-5p, miR-1225-5p, miR-1227-5p, miR-1228-3p, miR-1228-5p, miR-1237-5p, miR-1238-5p, miR-1247-3p, miR-1268a, miR-1268b, miR-1273g-3p, miR-128-2-5p, miR-1343-3p, miR-1343-5p, miR-1470, miR-17-3p, miR-187-5p, miR-1908-3p, miR-1908-5p, miR-1909-3p, miR-1915-3p, miR-210-5p, miR-24-3p, miR-2467-3p, miR-2861, miR-296-3p, miR-29b-3p, miR-3131, miR-3154, miR-3158-5p, miR-3160-5p, miR-3162-5p, miR-3178, miR-3180-3p, miR-3184-5p, miR-3185, miR-3194-3p, miR-3195, miR-3197, miR-320a, miR-320b, miR-328-5p, miR-342-5p, miR-345-3p, miR-3616-3p, miR-3619-3p, miR-3620-5p, miR-3621, miR-3622a-5p, miR-3648, miR-3652, miR-3656, miR-3663-3p, miR-3679-5p, miR-371b-5p, miR-373-5p, miR-3917, miR-3940-5p, miR-3960, miR-4258, miR-4259, miR-4270, miR-4286, miR-4298, miR-4322, miR-4327, miR-4417, miR-4419b, miR-4429, miR-4430, miR-4433a-3p, miR-4436b-5p, miR-4443, miR-4446-3p, miR-4447, miR-4448, miR-4449, miR-4454, miR-4455, miR-4459, miR-4462, miR-4466, miR-4467, miR-4480, miR-4483, miR-4484, miR-4485-5p, miR-4488, miR-4492, miR-4505, miR-4515, miR-4525, miR-4534, miR-4535, miR-4633-3p, miR-4634, miR-4640-5p, miR-4649-5p, miR-4651, miR-4652-5p, miR-4655-5p, miR-4656, miR-4658, miR-4663, miR-4673, miR-4675, miR-4687-3p, miR-4687-5p, miR-4690-5p, miR-4695-5p, miR-4697-5p, miR-4706, miR-4707-3p, miR-4707-5p, miR-4708-3p, miR-4710, miR-4718, miR-4722-5p, miR-4725-3p, miR-4726-5p, miR-4727-3p, miR-4728-5p, miR-4731-5p, miR-4736, miR-4739, miR-4740-5p, miR-4741, miR-4750-5p, miR-4755-3p, miR-4763-3p, miR-4771, miR-4783-3p, miR-4783-5p, miR-4787-3p, miR-4792, miR-498, miR-5008-5p, miR-5010-5p, miR-504-3p, miR-5195-3p, miR-550a-5p, miR-5572, miR-5739, miR-6075, miR-6076, miR-6088, miR-6124, miR-6131, miR-6132, miR-614, miR-615-5p, miR-619-5p, miR-642b-3p, miR-6510-5p, miR-6511a-5p, miR-6515-3p, miR-6515-5p, miR-663b, miR-6716-5p, miR-6717-5p, miR-6722-3p, miR-6724-5p, miR-6726-5p, miR-6737-5p, miR-6741-5p, miR-6742-5p, miR-6743-5p, miR-6746-5p, miR-6749-5p, miR-6760-5p, miR-6762-5p, miR-6765-3p, miR-6765-5p, miR-6766-3p, miR-6766-5p, miR-6771-5p, miR-6774-5p, miR-6777-5p, miR-6778-5p, miR-6780b-5p, miR-6781-5p, miR-6782-5p, miR-6784-5p, miR-6785-5p, miR-6787-5p, miR-6789-5p, miR-6791-5p, miR-6794-5p, miR-6800-5p, miR-6802-5p, miR-6803-5p, miR-6812-5p, miR-6816-5p, miR-6819-5p, miR-6821-5p, miR-6826-5p, miR-683-1-5p, miR-6836-3p, miR-6840-3p, miR-6842-5p, miR-6850-5p, miR-6861-5p, miR-6869-5p, miR-6870-5p, miR-6877-5p, miR-6879-5p, miR-6880-3p, miR-6880-5p, miR-6885-5p, miR-6887-5p, miR-7107-5p, miR-7108-3p, miR-7109-5p, miR-711, miR-7113-3p, miR-7150, miR-744-5p, miR-7975, miR-7977, miR-8052, miR-8069, miR-8073, miR-887-3p and miR-937-5p in the sample.

8. A method for detecting bladder cancer, comprising:
measuring an expression level of miR-1185-1-3p in a sample from a human subject;
determining that the level of miR-1185-1-3p in the sample from the human subject is increased in comparison to a level of miR-1185-1-3p in a control sample from a human subject without bladder cancer;
determining that the human subject has bladder cancer based on the increased expression level of miR-1185-1-3p; and
treating the human subject for the bladder cancer;
wherein the treating comprises surgery, radiotherapy, chemotherapy, or a combination thereof;
wherein the sample is blood, serum, or plasma; and
wherein the measuring is performed using a kit comprising a nucleic acid(s) capable of specifically binding to miR-1185-1-3p or a complement thereof.

9. The method according to claim 8 , wherein the method further comprises measuring an expression level(s) of at least one polynucleotide selected from miR-6087, miR-1185-2-3p, miR-1193, miR-1199-5p, miR-1225-5p, miR-1227-5p, miR-1228-3p, miR-1228-5p, miR-1237-5p, miR-1238-5p, miR-1247-3p, miR-1268a, miR-1268b, miR-1273¢-3p, miR-128-2-5p, miR-1343-3p, miR-1343-5p, miR-1470, miR-17-3p, miR-187-5p, miR-1908-3p, miR-1908-5p, miR-1909-3p, miR-1915-3p, miR-210-5p, miR-24-3p, miR-2467-3p, miR-2861, miR-296-3p, miR-29b-3p, miR-3131, miR-3154, miR-3158-5p, miR-3160-5p, miR-3162-5p, miR-3178, miR-3180-3p, miR-3184-5p, miR-3185, miR-3194-3p, miR-3195, miR-3197, miR-320a, miR-320b, miR-328-5p, miR-342-5p, miR-345-3p, miR-3616-3p, miR-3619-3p, miR-3620-5p, miR-3621, miR-3622a-5p, miR-3648, miR-3652, miR-3656, miR-3663-3p, miR-3679-5p, miR-371b-5p, miR-373-5p, miR-3917, miR-3940-5p, miR-3960, miR-4258, miR-4259, miR-4270, miR-4286, miR-4298, miR-4322, miR-4327, miR-4417, miR-4419b, miR-4429, miR-4430, miR-4433a-3p, miR-4436b-5p, miR-4443, miR-4446-3p, miR-4447, miR-4448, miR-4449, miR-4454, miR-4455, miR-4459, miR-4462, miR-4466, miR-4467, miR-4480, miR-4483, miR-4484, miR-4485-5p, miR-4488, miR-4492, miR-4505, miR-4515, miR-4525, miR-4534, miR-4535, miR-4633-3p, miR-4634, miR-4640-5p, miR-4649-5p, miR-4651, miR-4652-5p, miR-4655-5p, miR-4656, miR-4658, miR-4

Assignees

• Toray Industries
• Nat Cancer Ct

Inventors

• Yoshimoto Makiko
• Sudo Hiroko
• Kozono Satoko
• Kawauchi Junpei
• Ochiya Takahiro
• Fujimoto Hiroyuki
• USUBA Wataru
• Matsuzaki Juntaro

Classifications

• C12Q2600/158

  Expression markers · CPC title

• C12Q2600/178

  miRNA, siRNA or ncRNA · CPC title

• C12Q1/6837

  using probe arrays or probe chips (C12Q1/6874 takes precedence) · CPC title

• C12Q1/6886 Primary

  for cancer (immunoassay for cancer G01N33/575) · CPC title