High-throughput imaging platform

Patent metadata

Field	Value
Publication number	US-11726084-B2
Application number	US-202016747432-A
Country	US
Kind code	B2
Filing date	Jan 20, 2020
Priority date	Oct 27, 2014
Publication date	Aug 15, 2023
Grant date	Aug 15, 2023

Abstract

A microfluidic device capable of trapping contents in a manner suitable for high-throughput imaging is described herein. The microfluidic device may include one or more trapping devices, with each trapping device having a plurality of trapping channels. The trapping channels may be configured to receive contents via an inlet channel that connects a sample reservoir to the trapping channels via fluid communication. The trapping channels are shaped such that contents within the trapping channels are positioned for optimal imaging purposes. The trapping channels are also connect to at least one exit channel via fluid communication. The fluid, and contents within the fluid, may be controlled via hydraulic pressure.

First claim

What is claimed is:

1. A high-throughput microfluidic system comprising:
a microfluidic device comprising a plurality of trapping devices, the microfluidic device comprising at least one inlet and at least one exit through which fluid flows into and out of the microfluidic device, each trapping device comprising at least one inlet, a plurality of trapping channels, and an exit channel, the at least one inlet of each trapping device and the exit channel of the trapping device being in fluid communication with the plurality of trapping channels of the trapping device, and the at least one inlet of each trapping device being in fluid communication with the at least one inlet of the microfluidic device, wherein the exit channels of at least two of the plurality of trapping devices are in fluid communication with one another via an intermediate exit channel, and the intermediate exit channel is in fluid communication with the at least one exit of the microfluidic device;
a pressure device in fluid communication with the microfluidic device;
a valve in communication with the pressure device and the microfluidic device; and
a processor and a storage device, the storage device storing computer-readable instructions, and the processor configured for executing the computer-readable instructions, wherein the instructions cause the processor to control the valve and the pressure device to influence the amount of hydraulic pressure to be applied to the microfluidic device to position contents within the plurality of trapping channels, wherein the instructions cause the processor to cause the pressure device to apply pressure to a fluid and the instructions cause the processor to cause the valve to allow the pressurized fluid to flow through the microfluidic device in two or more cycles between 0 and 30 psi.

2. The high-throughput microfluidic system of claim 1 , further comprising a high-throughput imaging system, the high-throughput imaging system comprising:
a camera, an objective, and a motorized platform supporting the microfluidic device, and
a processor of the imaging system, wherein the processor of the imaging system is same as, or different from, the processor for controlling the valve, and a storage device, the storage device storing computer-readable instructions, and the processor configured for executing the computer-readable instructions, wherein the instructions cause the processor of the imaging system to:
receive parameter information regarding the microfluidic device, said parameter information comprising a well format, number of wells to be imaged, a desired number of z-stack images, and a z-step size;
measure, in three dimensions, the orientation of the microfluidic device;
calculate offsets in a focusing plane of the contents in the trapping devices;
calculate a curvature of a substrate of the microfluidic device for a given applied pressure;
move the motorized platform for focusing on the contents of the microfluidic device based on the measured orientation, calculated offsets, and calculated curvature;
acquire images of the contents in the microfluidic device as image files; and
store the image files.

3. The high-throughput microfluidic system of claim 2 , wherein the instructions cause the processor of the imaging system to obtain x, y, and z coordinates of a plurality of predetermined locations of the microfluidic device and cause the imaging system to identify the locations of the contents within the trapping channels within the predetermined locations of the microfluidic device.

4. The high-throughput microfluidic system of claim 2 , wherein instructions that cause the processor of the imaging system to acquire images comprise instructions that cause the processor of the imaging system to adjust camera exposure time, adjust a number of samples to image, adjust x- and y-locations, adjust x- and y-step sizes, adjust a number of z-stacks, and/or adjust a z-step size.

5. The high-throughput microfluidic system of claim 2 , wherein the processor for controlling the valve is the processor of the imaging system.

6. The high-throughput microfluidic system of claim 1 , wherein the microfluidic device comprises a vent to release air during application of hydraulic pressure from the pressure device.

7. The high-throughput microfluidic system of claim 1 , wherein each pressure cycle has a time period of 0 to 600 seconds.

Assignees

• Univ Texas

Inventors

• Ben-Yakar Adela
• Hegarty Evan
• Mondal Sudip
• Ghorashian Navid
• Gökçe Sertan Kutal
• Martin Christopher

Classifications

• G01N33/5085 Primary

  of invertebrates · CPC title

• B01L3/502715 Primary

  characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces · CPC title

• B01L3/502723

  characterised by venting arrangements · CPC title

• B01L3/502746

  characterised by the means for controlling flow resistance, e.g. flow controllers, baffles or throttle valves · CPC title

• B01L3/502761 Primary

  specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules · CPC title