Platform and method for testing antibiotic sensitivity of a polymicrobial infection

Patent metadata

Field	Value
Publication number	US-11725172-B2
Application number	US-201816217588-A
Country	US
Kind code	B2
Filing date	Dec 12, 2018
Priority date	Dec 12, 2018
Publication date	Aug 15, 2023
Grant date	Aug 15, 2023

Abstract

A platform and method for testing antibiotic sensitivity of a polymicrobial infection is provided. The platform includes a body defining a plurality of sets of chambers and a plurality of wells. Each set of chambers has a plurality of chambers adapted for culturing microbes of the polymicrobial infection therein. Each well is associated with a corresponding set of chambers and has an input in fluidic communication with the outlets of the plurality of chambers in the corresponding set of chambers. Selected antibiotic therapies may be received in the wells which fluidically connect the plurality of chambers in a corresponding set of chambers such that microbes cultured in the plurality of chambers in the corresponding set of chambers are in soluble factor contact.

First claim

We claim:

1. A platform for testing antibiotic sensitivity of a polymicrobial infection, comprising:
a body defining:
first and second chambers, each of the first and second chambers being adapted for receiving a corresponding microbe of the polymicrobial infection therein;
a well adapted for receiving a selected antibiotic therapy therein;
a first sidewall extending between the first and second chambers;
a second sidewall extending between the first chamber and the well; and
a third sidewall extends between the second chamber and the well;

wherein:
the first sidewall, the second sidewall, the third sidewall, the first and second chambers and the well are arranged to define a multiplexing chamber having an equilateral triangular configuration such that the first sidewall defines a first side of the multiplexing chamber, the second sidewall defines a second side of the multiplexing chamber; the third sidewall defines a third side of the multiplexing chamber, the first chamber defines a first vertex of the multiplexing chamber, the second chamber defines a second vertex of the multiplexing chamber and the well defines a third vertex of the multiplexing chamber; and
the selected antibiotic therapy received in the well and the microbes received in the first and second chambers are in soluble factor contact in the multiplexing chamber.

2. The platform of claim 1 wherein at least one of the first and second chambers includes an outlet and wherein the body further includes a permeable membrane disposed between the outlet of the at least one of the first and second chambers and the multiplexing chamber.

3. The platform of claim 2 wherein the permeable membrane is a porous polycarbonate membrane.

4. The platform of claim 1 wherein the body includes upper and lower surfaces, each of the first and second chambers having an opening communicating with the upper surface of the body.

Assignees

• Wisconsin Alumni Res Found

Inventors

• Beebe David J
• Barkal Layla J
• Yu Jason

Classifications

• C12M23/16 Primary

  Microfluidic devices; Capillary tubes (integrated microfluidic structures B01L3/5027; microreactors B01J19/0093) · CPC title

• C12M23/12

  Well or multiwell plates (C12M25/04 takes precedence) · CPC title

• C12Q1/18 Primary

  Testing for antimicrobial activity of a material · CPC title