Electrochemical detector

Patent metadata

Field	Value
Publication number	US-11719667-B2
Application number	US-201916668194-A
Country	US
Kind code	B2
Filing date	Oct 30, 2019
Priority date	Oct 30, 2019
Publication date	Aug 8, 2023
Grant date	Aug 8, 2023

Abstract

An electrochemical detector includes: a solution chamber and a substance selection structure separating the chamber into individual compartments, wherein the solution chamber is arranged to retain and separate solutions in each of the individual compartments; and a pair of electrodes each connecting the respective individual compartment, wherein the pair of electrodes is arranged to form a conductive path across the electrodes when in contact with the solutions retained in the solution chamber. The substance selection structure is arranged to interact with a target substance in the solution so as to alter an electrical characteristic of the conductive path defined by the pair of electrodes, the solution retained in the individual compartments in the solution chamber and the substance selection structure.

First claim

The invention claimed is:

1. An electrochemical detector comprising:
a solution chamber including a first portion and a second portion detachably connected with each other, the first and the second portions each has a compartment for containing a solution,
a pore and an opening is provided with the first portion, the pore and the opening are opened to the compartment of the first portion; the pore and the opening are physically separated from one another;
a pore and an opening is provided with the second portion, the pore and the opening are opened to the compartment in the second portion; the pore and the opening are physically separated from one another;
a pair of electrodes, one being inserted into the compartment of the first portion and the other being inserted into the compartment of the second portion via the respective pores, wherein the pair of electrodes is arranged to form a conductive path when in contact with the solutions retained in the compartments; and;
wherein a substance selection membrane comprises a porous gold deposited track-etched polycarbonate membrane having a pore size of about 50 nm, the membrane is functionalized thereon with a plurality of oligo-RNA receptors for binding with a gastric cancer antigen CA72-4 in the solution, thereby altering current flow in the conductive path; wherein the openings of both compartments are covered by the same substance selection membrane when the first and second portions are in connection with each other.

2. The electrochemical detector according to claim 1 , wherein the porous gold deposited track-etched polycarbonate membrane further includes a coupling agent arranged to couple the plurality of oligo-RNA receptors with the porous gold deposited track-etched polycarbonate membrane.

3. The electrochemical detector according to claim 2 , wherein the coupling agent includes a hydroxyl group forming a covalent bond with the plurality of oligo-RNA receptors.

4. The electrochemical detector according to claim 3 , wherein the coupling agent further includes a sulfhydryl group arranged to couple with the porous gold deposited track-etched polycarbonate membrane.

5. The electrochemical detector according to claim 4 , wherein the sulfhydryl group of the coupling agent forms a gold-sulphur bond with the porous gold deposited track-etched polycarbonate membrane.

6. The electrochemical detector according to claim 2 , wherein the coupling agent is 6-mercapto-1-hexanol.

7. The electrochemical detector according to claim 1 , wherein the plurality of oligo-RNA receptors forms a physical interaction with the gastric cancer antigen CA72-4.

8. The electrochemical detector according to claim 7 , wherein the plurality of oligo-RNA receptors forms at least one of electrostatic interactions, hydrophobic interactions or a complementary shape with the gastric cancer antigen CA72-4.

9. The electrochemical detector according to claim 7 , wherein formation of the physical interaction decreases a current flow of the conductive path.

10. The electrochemical detector according to claim 1 , wherein the solution chamber is an enclosed chamber.

11. The electrochemical detector according to claim 1 , wherein each of the first and the second portions includes an inlet, each connecting to the respective compartments of the chamber, for directing the solutions into the compartments.

12. The electrochemical detector according to claim 1 , wherein the chamber further includes an O-ring disposed between the compartments of the first and the second portions, thereby preventing solution leakage from the respective compartments.

13. The electrochemical detector according to claim 12 , wherein the O-ring is arranged on a side opposite to where the substance selection membrane interacts with the target substance.

14. The electrochemical detector according to claim 1 , wherein the deposited gold has a thickness of about 10 nm.

15. The electrochemical detector according to claim 1 , wherein the porous gold deposited track-etched polycarbonate membrane has a thickness of about 7 μm.

Assignees

• Univ City Hong Kong

Inventors

• Vellaisamy A L Roy
• Lau Chi Kong Terrence
• Saw Lin Oo

Classifications

• G01N33/5759

  involving compounds localised on the membrane of tumour or cancer cells · CPC title

• G01N33/5753 Primary

  of the stomach or small intestine · CPC title

• G01N27/31 Primary

  Half-cells with permeable membranes, e.g. semi-porous or perm-selective membranes · CPC title

• B01L3/502707

  characterised by the manufacture of the container or its components · CPC title

• G01N33/57446

  Physics · mapped topic