Biomarker detection system

Patent metadata

Field	Value
Publication number	US-11719632-B2
Application number	US-201716318946-A
Country	US
Kind code	B2
Filing date	Jul 21, 2017
Priority date	Jul 21, 2016
Publication date	Aug 8, 2023
Grant date	Aug 8, 2023

Abstract

Disclosed is a localised surface plasmon resonance (LSPR) nanopillar assembly. The LSPR assembly is for use in sensing the presence of a biomarker when attached to a quantum dot. The LSPR assembly comprises a substrate and an array. The array comprises a LSPR nanopillar and a polymer spacer attached to the nanopillar. The LSPR assembly further comprises an antibody attached to the at least one polymer spacer. In the LSPR assembly, a combined height of the polymer spacer and antibody is selected by varying the number of monomer units of the polymer spacer, so that, when in use with the biomarker and the quantum dot, the quantum dot is at a predetermined distance from the nanopillar.

First claim

The invention claimed is:

1. A localised surface plasmon resonance (LSPR) nanopillar assembly for use in sensing the presence of a biomarker when attached to a quantum dot, comprising:
a) a substrate;
b) an array comprising:
i) a plurality of metallic LSPR nanopillars, each of the nanopillars consists of a metallic nanopillar body; and
ii) at least one polymer spacer attached to the nanopillars; and

c) an antibody attached to the at least one polymer spacer;
wherein a combined height of the polymer spacer and antibody is such that, when in use with the biomarker and the quantum dot, the quantum dot is in an LSPR region of the nanopillar; and
each of the at least one polymer spacer comprises a discrete linear or non-linear polymer molecule having at least two opposed terminal ends, a first terminal end being attached to the nanopillar and a second spaced and opposed terminal end being attached to the antibody;
wherein the polymer spacer is a polyethylene glycol (PEG) spacer;
wherein the PEG spacer is PEG 2-100 ;
wherein the metallic nanopillar body has a length of about 50 nm to about 250 nm, width of about 50 nm to about 250 nm, and height of about 20 nm to about 100 nm;
wherein the nanopillar array comprises a plurality of metallic nanopillars, in which a pitch of the nanopillar array is about 150 nm to about 500 nm.

2. The LSPR nanopillar assembly of claim 1 , wherein the polymer spacer is conjugated to the metallic nanopillar body and the antibody is conjugated to the polymer spacer.

3. The LSPR nanopillar assembly of claim 1 , wherein the polymer spacer is maintained in a substantially upright position relative to a surface of the substrate to sense the presence of the biomarker when attached to the quantum dot.

4. The LSPR nanopillar assembly of claim 1 , wherein the polymer spacer is conjugated to the nanopillar by a sulphide bond.

5. The LSPR nanopillar assembly of claim 1 , wherein the PEG spacer is PEG 7 .

6. The LSPR nanopillar assembly of claim 1 , wherein the metallic nanopillar body has a length of about 140 nm, width of about 140 nm, and height of about 55 nm.

7. The LSPR nanopillar assembly of claim 1 , wherein the pitch of the metallic nanopillar array is about 320 nm.

8. The LSPR nanopillar assembly of claim 1 , wherein the metallic nanopillar body is substantially wholly made of gold or silver.

Assignees

• Temasek Polytechnic
• Agency Science Tech & Res
• Tan Tock Seng Hospital Pte Ltd

Inventors

• Sun Ling Ling
• Ng Willie
• Zhou Xiaodong
• Wong Ten It
• Leo Yee Sin

Classifications

• G01N21/554 Primary

  detecting the surface plasmon resonance of nanostructured metals, e.g. localised surface plasmon resonance · CPC title

• G01N21/01

  Arrangements or apparatus for facilitating the optical investigation · CPC title

• G01N21/648

  using evanescent coupling or surface plasmon coupling for the excitation of fluorescence · CPC title

• G01N33/54353

  with ligand attached to the carrier via a chemical coupling agent (coatings G01N33/54393) · CPC title

• G01N33/54373

  involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings · CPC title